Identification and characterization of the rice pre‐harvest sprouting mutants involved in molybdenum cofactor biosynthesis

Liu, Xin; Wang, Jing; Yu, Yang; Kong, Lina; Liu, Yimei; Liu, Zhiqi; Li, Hongyu; Wei, Piwei; Liu, Minglong; Zhou, Hai; Bu, Qingyun; Fang, Jun

doi:10.1111/nph.15607

Cited by 22 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although many quantitative trait loci (QTLs) or genetic loci associated with PHS have been isolated in rice [30][31][32][33][34], the number of cloned genes is limited and the molecular mechanism that underlies PHS remains elusive. Most of the reported genes relate to ABA metabolism, such as OsABA1, OsPLA3, PHS1 to PHS4, and OsCNX6 [5,27,35,36]. Recently, several studies have revealed some regulatory modules involved in PHS.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Zhu¹,

Wang²,

Lu³

et al. 2021

IJMS

View full text Add to dashboard Cite

Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of OsbZIP09 inhibited rice PHS. Analysis of the expression of OsbZIP09 and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including OsLOX2 and Late Embryogenesis Abundant (LEA) family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and OsbZIP09 mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Zhu¹,

Wang²,

Lu³

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Under drought condition, drought was simulated by adding polyethylene glycol‐6000 (PEG‐6000, same amounts as for control conditions) 15% in nutrient solution equivalent to a threshold of −0.295 Mpa (Mahesh et al., 2013). PEG has been frequently used in simulating artificial drought stresses due to its effect on water holding capacity (e.g.PEG‐6000 at 15% as threshold drought stress level was used in recent studies, An et al., 2016; Lee et al., 2020; Liu et al, 2019; Mahesh et al., 2013). Ten days after treatments start, half of the plants in each treatment (three pots) were sampled for biomass estimation; the remaining plants were grown under control conditions after the end of treatments.…”

Section: Methodsmentioning

confidence: 99%

Variation in quinoa roots growth responses to drought stresses

Nguyen

Bertero

Hoang

et al. 2021

J Agronomy Crop Science

View full text Add to dashboard Cite

Soil moisture stress has become a serious environmental limitation to crop productivity and quality. The root system is the first organ sensing the changes in soil moisture; therefore, root development under water deficit is an important indicator for plant's drought tolerance. Previous studies focused on quinoa varietal differences in morphological traits under water stress; however, variation in root development including both growth and diameter responses to drought remains largely unclear. We conducted a preliminary screening of a diverse set of 30 quinoa genotypes to evaluate genetic variation in growth and yield performance in response to drought stress.Variation in drought tolerance indices showed large variation across the quinoa collection. Based on these results, five genotypes representative of a range of drought tolerance levels including 2-Want, Atlas, NL-6, Pichamán and Sayaña were selected to evaluate root development under control and severe drought conditions. Inhibition of root development was found for all genotypes as compared to controls; however, significant variation in root growth response to drought stress was observed. Among genotypes, Atlas and 2-Want expressed drought-tolerant phenotypes. The analysis of the interrelations between genotypes root length, root diameter, root surface area, drought tolerance and geographical origins reveals interesting guidelines for further studies to explore the mechanisms behind quinoa roots adaptation to drought.

show abstract

“…After-ripening terjadi setelah benih mencapai masak fisiologis dan masih berada pada tanaman induk, disebut sebagai dormansi primer atau innate dormancy. Kandungan ABA dalam benih meningkat selama proses perkembangan benih, seiring dengan meningkatnya kemasakan benih, sebaliknya kandungan GA 3 dalam benih semakin menurun (Liu et al, 2019). Ketidak-seimbangan ABA dan GA 3 dalam benih ditengarai sebagai salah satu penyebab terjadinya dormansi after-ripening pada benih padi (Oracz dan Karpinski, 2016).…”

Section: Pendahuluanunclassified

Karakter Agro-morfologi dan Periode After-ripening Benih Padi Lanras Lokal Potensial asal Timor-Leste

BRANCO

Ilyas

Purwoko

et al. 2022

J. Agron. Indonesia

View full text Add to dashboard Cite

Benih padi yang baru dipanen umumnya tidak berkecambah walaupun dikecambahkan pada kondisi optimum yang disebut after-ripening, dan secara alamiah akan hilang setelah waktu tertentu. Penelitian ini bertujuan melakukan karakterisasi agro-morfologi dan menetapkan periode after-ripening benih 5 lanras potensial di Timor-Leste. Penelitian dilaksanakan pada bulan April 2018-November 2019. Penanaman dan pengamatan karakter agro-morfologi 5 lanras potensial Timor-Leste, yaitu Hare R-oitu, Fos Mean, Hare Belit, Hare Nona Portu, dan Ale Mamea Ula Lesa, dilaksanakan di Desa Caibada Distrik Baucau, Timor-Leste. Pengamatan periode after ripening dan mutu benih ke lima lanras tersebut dilaksanakan di Laboratorium Pengujian dan Penyimpanan Benih, Departemen Agronomi dan Hortikultura, IPB University, dan dirancang dalam rancangan acak lengkap (RAL) dua faktor dengan empat ulangan. Faktor pertama adalah 5 lanras tersebut di atas dan faktor kedua adalah periode simpan yang terdiri atas 2, 3, 4, 5, 6, 7, dan 8 minggu setelah panen (MSP). Hasil penelitian menunjukkan bahwa 5 lanras potensial di Tinmor-Leste mempunyai keragaan tanaman yang pendek (76.3-105.50 cm), tergolong berumur sedang (129-141 HSS), dan jumlah anakan produktif tinggi (17.5-53.50 anakan), kecuali Ale Mamea Ula Lesa. Lima lanras tersebut mempunyai potensi produktivitas yang tinggi berkisar 8.1-9.8 ton ha-1. Periode after-ripening lanras Hare Belit dan Hare Nona Portu berakhir pada 2 MSP, Hare R-oitu pada 6 MSP, sedangkan Ale Mamea Ula Lesa dan Fos Mean pada 8 MSP. Semakin lama benih disimpan rasio kandungan ABA/GA3 semakin menurun dari 0.53-1.22 ppm pada 2 MSP menjadi 0.45-0.93 ppm pada 4 MSP. Lanras Hare Belit dan Hare Nona Portu merupakan lanras yang potensial dikembangkan menjadi varietas nasional di Timor-Leste, masing-masing untuk pengembangan di dataran rendah dan dataran sedang. Kata kunci: karakterisasi, intensitas dormansi, persentase perkecambahan, mutu benih

show abstract

Identification and characterization of the rice pre‐harvest sprouting mutants involved in molybdenum cofactor biosynthesis

Cited by 22 publications

References 53 publications

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Variation in quinoa roots growth responses to drought stresses

Karakter Agro-morfologi dan Periode After-ripening Benih Padi Lanras Lokal Potensial asal Timor-Leste

Contact Info

Product

Resources

About