Identification and Biological Characterization ofLeishmania (Viannia) guyanensisIsolated from a Patient with Tegumentary Leishmaniasis in Goiás, a Nonendemic Area for This Species in Brazil

Abstract

The aim of this study was to characterize clinical field isolates of Leishmania spp. obtained from patients with American Tegumentary Leishmaniasis (ATL) who live in Goiás state, Brazil. The presumed areas of infection were in Goiás, Tocantins, and Pará states. Three isolates of parasites were identified as L. (Viannia) braziliensis and one as L. (V.) guyanensis. The in vitro growth profiles were found to be similar for all parasites. Nevertheless, in C57BL/6 mice, L. (V.) guyanensis infection was better controlled than L. (V.) braziliensis. Yet in C57BL/6 mice deficient in interferon gamma, L. (V.) guyanensis lesions developed faster than those caused by L. (V.) braziliensis isolates. In BALB/c mice, the development of lesions was similar for isolates from both species; however, on the 11th week of infection, amastigotes could not be observed in macrophages from L. (V.) guyanensis-infected mice. Thus, L. (V.) guyanensis can be circulating in Goiás, a state where autochthonous cases of this species had not yet been reported. Considering the difficulties to differentiate L. (V.) guyanensis from L. (V.) braziliensis at the molecular, morphological, and clinical (human and murine models) levels, the presence of L. (V.) guyanensis infections is possibly underestimated in several regions of Brazil.

Metacyclogenesis ofLeishmania (Viannia) guyanensis: a comprehensive study of the main transformation features in axenic culture and purification of metacyclic promastigotes by negative selection withBauhinia purpurealectin

“…In a recent study, Pires et al . (2015), reporting the characterization of an isolate of L. guyanensis from a patient in Goiás, Brazil, emphasized the crucial importance of correct identification and characterization of species of the subgenus Leishmania Viannia , suggesting that the presence of L. guyanensis may be underestimated in endemic regions. Despite the epidemiologic importance of L. guyanensis , in PubMed web site (), as accessed 12 December 2018, of the 13 134 hits containing the keyword Leishmania in the title of the article, only 93 (0.71%) contains the words Leishmania and guyanensis , demonstrating the remarkable lack of data concerning this important species.…”

“…In a recent study, Pires et al (2015), reporting the characterization of an isolate of L. guyanensis from a patient in Goiás, Brazil, emphasized the crucial importance of correct identification and characterization of species of the subgenus Leishmania Viannia,…”

Case Report: Atypical Cutaneous Leishmaniasis in a Patient with Mixed Leishmania guyanensis and Leishmania amazonensis Infection

“…Two different patterns of digestion were obtained that were sequenced as described. 6 Sequence analysis of one clone representative of each one of the two patterns indicated the presence of one sequence 99% identical with the reference strain Leishmania (Leishmania) amazonensis (MHOM/BR/ 73M2269), whereas the second profile corresponded to a sequence presenting 98% identity with the reference strain Leishmania (Viannia) guyanensis (MHOM/BR/75/M4147). The comparison between sequences of these two profiles revealed 62% identity ( Figure 3).…”

Epidemiologia da leishmaniose tegumentar americana (LTA) no município de Xapuri, Estado do Acre, Brasil: estudo em população humana, cães domésticos e vetores

“…(V.) braziliensis não são bons reservatórios deste parasito (MADEIRA et al, 2005(MADEIRA et al, , 2006CASTRO et al, 2007) LAINSON et al 1994;AZEVEDO et al, 2002, TOJAL et al, 2003, CÓRDOVA et al, 2011BRASIL, 2013;PIRES et al, 2015;TELES et al, 2016;KOARASHI et al, 2016 A espécie Ny. umbratilis (Ward & Fraiha, 1977), principal vetor suspeito, espécie com ampla distribuição na Amazônia, podendo ser encontrada em florestas primárias, principalmente em troncos de árvores, e que são altamente antropofílicas (LAINSON et al, 1994 Peru (LUCAS et al, 1994;MARTINEZ et al, 2001;TOJAL et al, 2006;TELES et al, 2015) e recentemente no Equador (KATO et al, 2016).…”

“…Estes testes são bem empregados em controle de cura da doença, após o tratamento (SOUZA et al, 1982). Em áreas onde a LV é endêmica, têm sido observadas reações cruzadas com doença de Chagas, por serem parasitos do mesmo grupo específico (MATOS et al, 2015). O Ministério da Saúde recomenda que não seja utilizada a técnica de RIFI como critério de confirmação do diagnóstico de LTA, pelo seu limitado valor diagnóstico, devendo ser associada a outras técnicas diagnósticas (BRASIL, 2013).…”