Identification and antagonistic activity of endophytic bacterial strain Paenibacillus sp. 5 L8 isolated from the seeds of maize (Zea mays L., Jingke 968)

Abstract

Endophytes are related to the health and growth of plants. In this study, the endophytic bacterial strain Paenibacillus sp. 5 L8 was isolated from the seeds of maize (Zea mays L., Jingke 968). Strain 5 L8 was identified as Paenibacillus polymyxa by polyphasic taxonomy based on morphological and physiological methods, and 16S rRNA gene phylogenetic analysis. Via antagonistic testing against the common plant pathogens Fusarium graminearum, Bipolaris maydis, Bipolaris sorokiniana, Cochliobolus heterostrophus, Aspergillus aculeatus, Phomopsis chimonanthi and Verticillium dahliae, strain 5 L8 showed comprehensive and good antagonistic activity. The draft genome of Paenibacillus sp. 5 L8 was sequenced by Illumina HiSeq 2000, and three coding sequences (CDSs) for the glucanase gene were annotated and correlated to antagonistic activity. Using specific primers to amplify the biocontrol gene in the glucanase family, the β-1,3-1,4-glucanase gene (gluB) was found. This study supplies a basis for further study of industrial research and application.

Investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize (Zea mays L.) genotypes

“…5L8 isolated from the seeds of maize (Zea mays L., Jingke 968) had comprehensive and effective antagonistic activity against the common plant pathogens Fusarium graminearum, Bipolaris maydis, Bipolaris sorokiniana etc. (Liu et al 2016a). High-throughput sequencing (HTS) contributes to gain more insight into rich endophytic bacteria in maize seeds, Pantoea, Enterobacter, Aeribacillus, Sphingomonas and Halomonas, were identified as the dominant species in different hybrid maize combinations (Chen et al 2016;Liu et al 2017a).…”

A High-Throughput Screening Method for Endophytic Bacteria With Antagonistic Activity Against Magnaporthe Oryzae in Rice (Oryza Sativa L.) Seeds

“…In the past, our research group used traditional methods to screen and explore the endophytic bacteria with antagonistic activity against pathogenic fungi in plant seeds, and the entire experimental process took at least one week or more (Jing et al 2020;Yang et al 2020). By consulting the method description in other related reports, we can also nd that it takes a long time from strain screening to the completion of antagonistic experiments (Liu et al 2016 et al 2020). Compared with the traditional strain screening and plate confrontation experiment, the onestep high-throughput screening method for endophytic bacteria with antagonistic activity against pathogenic fungi in rice seeds can complete all the work in four or ve days.…”

Marine yeasts and bacteria as biological control agents against anthracnose on mango

“…The enzymatic activity of microorganisms is considered as another antagonistic mechanism. Enzymes as glucanase, protease and chitinase are related directly with degradation of β‐glucan, manoprotein and chitin, respectively, which are main components of the cell wall of phytopathogen fungi (Liu et al., ; Vero, Garmendia, González, & Bentancur, ). Resistance induction of the host is another antagonistic mechanism by which yeasts and bacteria indirectly limit growth of phytopathogenic fungi in the host (Wei, Xu, Wu, & Tu, ).…”

“…Among the potential antagonists, yeasts and bacteria have received attention as potential biocontrol agents against postharvest diseases in fruit, mainly because of their high inhibitory capacity, rapid colonization of fruit wounds and simple nutritional requirements (Aloui, Licciardello, Khwaldia, Hamdi, & Restuccia, ; Liu, Wang, Cao, & Chen, ). In addition, these potential biocontrol agents are a major component of the epiphytic microbial community on fruit surfaces and are also phenotypically adapted to this niche (Parafati, Vitale, Restuccia, & Cirvilleri, ).…”