A High-Throughput Screening Method for Endophytic Bacteria With Antagonistic Activity Against Magnaporthe Oryzae in Rice (Oryza Sativa L.) Seeds

Abstract

Screening for microorganisms with antagonistic activity against pathogens in crops is of great significance for the preparation of microbial antagonists, the control of crop diseases, and the sustainable development of agriculture. Through the experiment, a rapid, efficient, and one-step high-throughput method for screening endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice seeds was successfully established, and the experimental results showed that the endophytic bacteria with antagonistic activity of Magnaporthe oryzae ACCC 36020 could be directly screened from six groups of experimental samples by this method. The establishment of this method can achieve simultaneous screening and purification, one-step high-throughput screening of antagonistic bacteria in rice seeds, and greatly save time.