ICP-MS as a new tool for the determination of gold nanoparticles in bioanalytical applications

Scheffer, Andy; Engelhard, Mark H.; Sperling, Michael; Büscher, Wolfgang

doi:10.1007/s00216-007-1576-5

Cited by 94 publications

(55 citation statements)

References 9 publications

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“…[158] A number of reports on the use of ICP for the characterization of colloidal systems can be found in literature. While some reports describe the elemental analysis of synthetic, inorganic particles, [161][162][163][164] most of the literature reported for ICP characterization of colloids deals with slurry methods for the analysis of sediments for environmental sciences. [155][156][157] Investigations on polymer-based hybrid nanoparticles are rare in literature.…”

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confidence: 99%

Surface Patterning with Colloidal Monolayers

Vogel

2012

Springer Theses

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This thesis focuses on the controlled assembly of monodisperse polymer colloids into ordered two-dimensional arrangements. These assemblies, commonly referred to as colloidal monolayers, are subsequently used as masks for the generation of arrays of complex metal nanostructures on solid substrates.The motivation of the research presented here is twofold. First, monolayer crystallization methods were developed to simplify the assembly of colloids and to produce more complex arrangements of colloids in a precise way. Second, various approaches to colloidal lithography are designed with the aim to include novel features or functions to arrays of metal nanostructures.The air/water interface was exploited for the crystallization of colloidal monolayer architectures as it combines a two-dimensional confinement with a high lateral mobility of the colloids that is beneficial for the creation of high long range order. A direct assembly of colloids is presented that provides a cheap, fast and conceptually simple methodology for the preparation of ordered colloidal monolayers. The produced two-dimensional crystals can be transformed into nonclose-packed architectures by a plasma-induced size reduction step, thus providing valuable masks for more sophisticated lithographic processes. Finally, the controlled co-assembly of binary colloidal crystals with defined stoichiometries on a Langmuir trough is introduced and characterized with respect to accessible configurations and size ratios.Several approaches to lithography are presented that aim at introducing different features to colloidal lithography. First, using metal-complex containing latex particles, the synthesis of which is described as well, symmetric arrays of metal nanoparticles can be created by controlled combustion of the organic material of the colloids. The process does not feature an inherent limit in nanoparticle size and is able to produce complex materials as will be demonstrated for FePt alloy particles. Precise control over both size and spacing of the particle array is presented.Second, two lithographic processes are introduced to create sophisticated nanoparticle dimer units consisting of two crescent shaped nanostructures in close proximity; essentially by using a single colloid as mask to generate two structures simultaneously. Strong coupling processes of the parental plasmon resonances of the two objects are observed that are accompanied by high near-field enhancements. A plasmon hybridization model is elaborated to explain all polarization dependent shifts of the resonance positions. Last, a technique to produce laterally patterned, ultra-flat substrates without surface topographies by embedding gold nanoparticles in a silicon dioxide matrix is applied to construct robust and re-usable sensing architectures and to introduce an approach for the nanoscale patterning of solid supported lipid bilayer membranes.

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confidence: 99%

Surface Patterning with Colloidal Monolayers

Vogel

2012

Springer Theses

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“…The single-particle ICP-MS analysis has been tested for nanoparticles of TiO 2 , Al 2 O 3 , ZrO 2 , ThO 2 , and Au-NPs in water [26 -30]. When the nanoparticles were used as tags in bioassay, this single-particle mode ICP-MS analysis affords the possibility of developing highly sensitive immunoassay and nucleic acids analysis strategies [31].…”

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confidence: 99%

A new strategy for highly sensitive immunoassay based on single-particle mode detection by inductively coupled plasma mass spectrometry

Liu

Zhang

et al. 2009

J. Am. Soc. Mass Spectrom.

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A highly sensitive immunoassay is proposed based on time-resolved inductively coupled plasma mass spectrometry with nanoparticles as tags to antibody. Instead of using traditional integral mode detection, the transient signals induced by the flash of ions in the plasma torch from the ionization of nanoparticles tagged on antibody were recorded in a time-resolved mode. Since, under certain conditions, the frequency of transient signals is directly correlated to the concentration of nanoparticle tags, the concentration of nanoparticle-tagged antibodies can be quantified by the frequency of transient signals. With the present instrument setup, gold nanoparticle (Au-NP) tags, as small as about 15 nm in diameter, can be detected. This protocol is evaluated for a competitive immunoassay and the linear range for ␣-fetoprotein is 0.016 -6.8 g/L (between 20 and 80% inhibition). The limit of quantification is 0.016 g/L (20% inhibition, IC 20 ) with a relative standard deviation of 4.2% (20% inhibition, 4 replicates) for ␣-fetoprotein. The present strategy provides a sensitive readout method for nanoparticle tags, which is quite promising for numerous applications in immunoassay, DNA hybridization, and other biological analyses. (J Am Soc Mass

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“…The size and morphology of AuNPs were examined by transmission electron microscopy (TEM, H-7500, Hitachi, Japan) at 80 kV. The concentration of AuNPs supernatant solution was measured by inductively coupled plasma mass spectrometry (ICP-MS, VG Plasma Quad 3, UK), described by Scheffer et al [17].…”

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confidence: 99%

Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity

Lin

Yao

et al. 2013

Chin. Sci. Bull.

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Gold nanoparticles (AuNPs) have been reported to induce faster heat transfer of polymerase chain reaction (PCR) solution to enhance PCR efficiency. AuNPs can also increase the specificity of PCR by functioning analogously to single-stranded DNA binding protein (SSB). However, the simple structure of AuNPs makes it difficult to determine how AuNPs affect PCR efficiency and specificity. This study aimed to elucidate the effect of AuNPs on PCR efficiency by altering the denaturation times and annealing temperatures. In addition, comparative study of the effect of AuNPs on PCR inhibition caused by two competitive primers allowed investigation of the mechanisms of AuNP-enhanced PCR accuracy. Finally, heat-treated salmon sperm DNA was used to evaluate whether AuNPs could eliminate the inhibitory PCR effect caused by DNA impurities. This study demonstrated that enhanced thermal conductivity by AuNPs was the main mechanism for increased PCR efficiency and specificity. AuNPs promoted efficient double-stranded DNA template unwinding and dissociation between mismatched primers, DNA fragments and template, and enhanced PCR efficiency and specificity simultaneously. Thus, this significant finding suggests the future use of AuNP-assisted PCR in different fields, especially in rapid clinical diagnosis and screening by PCR.

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ICP-MS as a new tool for the determination of gold nanoparticles in bioanalytical applications

Cited by 94 publications

References 9 publications

Surface Patterning with Colloidal Monolayers

Surface Patterning with Colloidal Monolayers

A new strategy for highly sensitive immunoassay based on single-particle mode detection by inductively coupled plasma mass spectrometry

Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity

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