<i>TFT6</i> and <i>TFT7</i>, two different members of tomato 14‐3‐3 gene family, play distinct roles in plant adaption to low phosphorus stress

Xu, Weifeng; Shi, Weiming; Jia, Liguo; Liang, Jiansheng; Zhang, Jianhua

doi:10.1111/j.1365-3040.2012.02497.x

Cited by 72 publications

(61 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous results (Xu et al, 2012b) demonstrated that TFT7 directly functions in the root by enhancing H + secretion under low-phosphorus stress. Therefore, we asked whether TFT7 is involved under alkaline stress.…”

Section: Different Responses Of Tft4 and Tft7mentioning

confidence: 87%

“…In higher plants, 14-3-3 proteins are encoded by a multigene family and play important roles in regulating plant development and stress responses (Mayfield et al, 2012). Although 14-3-3 proteins in plants possess a highly conserved target-binding domain, several studies indicate that various 14-3-3 isoforms may regulate different targets or act in distinct locations under variable abiotic stresses (Sehnke et al, 2002;Xu et al, 2012b). At least 12 genes predicted to encode 14-3-3 proteins (TOMATO 14-3-3 PROTEIN1 [TFT1]-TFT12) have been identified in tomato (Solanum lycopersicum; Roberts, 2003;Xu and Shi, 2006).…”

mentioning

confidence: 99%

See 1 more Smart Citation

The Tomato 14-3-3 Protein TFT4 Modulates H+ Efflux, Basipetal Auxin Transport, and the PKS5-J3 Pathway in the Root Growth Response to Alkaline Stress

Jia

Shi

et al. 2013

Plant Physiology

Self Cite

View full text Add to dashboard Cite

Alkaline stress is a common environmental stress, in particular in salinized soils. Plant roots respond to a variety of soil stresses by regulating their growth, but the nature of the regulatory pathways engaged in the alkaline stress response (ASR) is not yet understood. Previous studies show that PIN-FORMED2, an auxin (indole-3-acetic acid [IAA]) efflux transporter, PKS5, a protein kinase, and DNAJ HOMOLOG3 (J3), a chaperone, play key roles in root H + secretion by regulating plasma membrane (PM) H + -ATPases directly or by targeting 14-3-3 proteins. Here, we investigated the expression of all 14-3-3 gene family members (TOMATO 14-3-3 PROTEIN1 [TFT1]-TFT12) in tomato (Solanum lycopersicum) under ASR, showing the involvement of four of them, TFT1, TFT4, TFT6, and TFT7. When these genes were separately introduced into Arabidopsis (Arabidopsis thaliana) and overexpressed, only the growth of TFT4 overexpressors was significantly enhanced when compared with the wild type under stress. H + efflux and the activity of PM H + -ATPase were significantly enhanced in the root tips of TFT4 overexpressors. Microarray analysis and pharmacological examination of the overexpressor and mutant plants revealed that overexpression of TFT4 maintains primary root elongation by modulating PM H + -ATPase-mediated H + efflux and basipetal IAA transport in root tips under alkaline stress. TFT4 further plays important roles in the PKS5-J3 signaling pathway. Our study demonstrates that TFT4 acts as a regulator in the integration of H + efflux, basipetal IAA transport, and the PKS5-J3 pathway in the ASR of roots and coordinates root apex responses to alkaline stress for the maintenance of primary root elongation.

show abstract

Section: Different Responses Of Tft4 and Tft7mentioning

confidence: 87%

mentioning

confidence: 99%

The Tomato 14-3-3 Protein TFT4 Modulates H+ Efflux, Basipetal Auxin Transport, and the PKS5-J3 Pathway in the Root Growth Response to Alkaline Stress

Jia

Shi

et al. 2013

Plant Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…In tomato, there are 12 genes predicted to encode 14-3-3 proteins, which are named in the sequence TFT1 to TFT12 (Xu and Shi, 2006). The tomato 14-3-3 family can be divided into two major groups, the non-« group (TFT1-TFT6, TFT10, and TFT11) and an «-like group (TFT7-TFT9 and TFT12; Xu et al, 2012). As TFT1 and TFT5 are both members of the non-« group, it is possible that isoform specificity exists for the HopQ1 interaction, potentially influenced by TFT1's and TFT5's subcellular localization and expression pattern in leaves.…”

Section: Discussionmentioning

confidence: 99%

The Pseudomonas syringae Effector HopQ1 Promotes Bacterial Virulence and Interacts with Tomato 14-3-3 Proteins in a Phosphorylation-Dependent Manner

Yadeta

Elmore

et al. 2013

Plant Physiology

View full text Add to dashboard Cite

A key virulence strategy of bacterial pathogens is the delivery of multiple pathogen effector proteins into host cells during infection. The Hrp outer protein Q (HopQ1) effector from Pseudomonas syringae pv tomato (Pto) strain DC3000 is conserved across multiple bacterial plant pathogens. Here, we investigated the virulence function and host targets of HopQ1 in tomato (Solanum lycopersicum). Transgenic tomato lines expressing dexamethasone-inducible HopQ1 exhibited enhanced disease susceptibility to virulent Pto DC3000, the Pto ƊhrcC mutant, and decreased expression of a pathogen-associated molecular pattern-triggered marker gene after bacterial inoculation. HopQ1-interacting proteins were coimmunoprecipitated and identified by mass spectrometry. HopQ1 can associate with multiple tomato 14-3-3 proteins, including TFT1 and TFT5. HopQ1 is phosphorylated in tomato, and four phosphorylated peptides were identified by mass spectrometry. HopQ1 possesses a conserved mode I 14-3-3 binding motif whose serine-51 residue is phosphorylated in tomato and regulates its association with TFT1 and TFT5. Confocal microscopy and fractionation reveal that HopQ1 exhibits nucleocytoplasmic localization, while HopQ1 dephosphorylation mimics exhibit more pronounced nuclear localization. HopQ1 delivered from Pto DC3000 was found to promote bacterial virulence in the tomato genotype Rio Grande 76R. However, the HopQ1(S51A) mutant delivered from Pto DC3000 was unable to promote pathogen virulence. Taken together, our data demonstrate that HopQ1 enhances bacterial virulence and associates with tomato 14-3-3 proteins in a phosphorylation-dependent manner that influences HopQ1’s subcellular localization and virulence-promoting activities in planta.

show abstract

“…8,9 Further, using transgenic Arabidopsis plants, SIET (scanning ion-selective electrode technique) and grafting technique, we found that TFT6 (one member of non-ε group in tomato 14-3-3 family) is the later responsive gene, and TFT6 which acts mainly in leaves is involved in the systemic response to low-phosphorus-stress by regulating leaf carbon allocation and increasing phloem sucrose transport to promote root growth, while TFT7 (one member of ε-like group in tomato 14-3-3 family) is the early responsive gene and directly functions in root by activating root plasma membrane H + -ATPase to release more protons under low-phosphorus-stress. 10 These results including genetic data indicate that different plant 14-3-3 isoforms have the distinct functions under phosphate deficiency. Thus, owe to the isoform-specificity, plant 14-3-3 proteins play the smart role in response to phosphate deficiency.…”

Section: Adaption Of 14-3-3 Proteins To Phosphate Deficiencymentioning

confidence: 84%

Smart role of plant 14-3-3 proteins in response to phosphate deficiency

Jia

Shi

et al. 2012

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

TFT6 and TFT7, two different members of tomato 14‐3‐3 gene family, play distinct roles in plant adaption to low phosphorus stress

Cited by 72 publications

References 54 publications

The Tomato 14-3-3 Protein TFT4 Modulates H+ Efflux, Basipetal Auxin Transport, and the PKS5-J3 Pathway in the Root Growth Response to Alkaline Stress

The Tomato 14-3-3 Protein TFT4 Modulates H+ Efflux, Basipetal Auxin Transport, and the PKS5-J3 Pathway in the Root Growth Response to Alkaline Stress

The Pseudomonas syringae Effector HopQ1 Promotes Bacterial Virulence and Interacts with Tomato 14-3-3 Proteins in a Phosphorylation-Dependent Manner

Smart role of plant 14-3-3 proteins in response to phosphate deficiency

Contact Info

Product

Resources

About