Smart role of plant 14-3-3 proteins in response to phosphate deficiency

Xu, Weifeng; Jia, Liguo; Shi, Weiming; Liang, Jiansheng; Zhang, Jianhua

doi:10.4161/psb.20997

Cited by 9 publications

(6 citation statements)

References 10 publications

(10 reference statements)

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“…Recently, the ectopic overexpression of the cassava 14-3-3 gene in Arabidopsis showed an increase in starch content in the leaves from transgenic plants [ 36 ]. A striking feature of the 14-3-3 proteins is their ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors [ 41 ], and 14-3-3 proteins play roles in regulating plant development and stress responses by effecting direct protein-protein interactions [ 42 , 43 , 44 ]. The interactions with 14-3-3s are subject to environmental control through signaling pathways that impact on 14-3-3 binding sites.…”

Section: Resultsmentioning

confidence: 99%

Proteomics Analysis Reveals Non-Controlled Activation of Photosynthesis and Protein Synthesis in a Rice npp1 Mutant under High Temperature and Elevated CO2 Conditions

Inomata

Baslam

Masui

et al. 2018

IJMS

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Rice nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) catalyzes the hydrolytic breakdown of the pyrophosphate and phosphodiester bonds of a number of nucleotides including ADP-glucose and ATP. Under high temperature and elevated CO2 conditions (HT + ECO2), the npp1 knockout rice mutant displayed rapid growth and high starch content phenotypes, indicating that NPP1 exerts a negative effect on starch accumulation and growth. To gain further insight into the mechanisms involved in the NPP1 downregulation induced starch overaccumulation, in this study we conducted photosynthesis, leaf proteomic, and chloroplast phosphoproteomic analyses of wild-type (WT) and npp1 plants cultured under HT + ECO2. Photosynthesis in npp1 leaves was significantly higher than in WT. Additionally, npp1 leaves accumulated higher levels of sucrose than WT. The proteomic analyses revealed upregulation of proteins related to carbohydrate metabolism and the protein synthesis system in npp1 plants. Further, our data indicate the induction of 14-3-3 proteins in npp1 plants. Our finding demonstrates a higher level of protein phosphorylation in npp1 chloroplasts, which may play an important role in carbohydrate accumulation. Together, these results offer novel targets and provide additional insights into carbohydrate metabolism regulation under ambient and adverse conditions.

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Section: Resultsmentioning

confidence: 99%

Proteomics Analysis Reveals Non-Controlled Activation of Photosynthesis and Protein Synthesis in a Rice npp1 Mutant under High Temperature and Elevated CO2 Conditions

Inomata

Baslam

Masui

et al. 2018

IJMS

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“…In the “NM + WD” treatment, the expression levels of PcGRF1 , PcGRF2 , PcGRF3 , PcGRF4 , PcGRF5 , PcGRF6 , PcGRF7 , PcGRF8 , and PcGRF9 in leaves, and PcGRF1 , PcGRF2 , PcGRF3 , and PcGRF5 in roots decreased with the increase of P addition levels, indicating that more 14-3-3’s expression was induced in plants under the dual stress of drought and low P ( Huang et al, 2021 ). These results indicated that 14-3-3 proteins play a smart role in response to abiotic stress in plants ( Xu et al, 2012a ), but the expression of these genes did not have much effect on drought resistance. Our results showed that the relative expression levels of PcGRF10 , PcGRF11 , and PcGRF12 in leaves, and PcGRF10 and PcGRF11 in roots were significantly higher under the WD condition than the WW, and their expression levels increased with the increase of P addition, which may help plants to absorb more P. The PcGRF10 and PcGRF11 expression levels of the “AM + WD” group in leaves were significantly higher than the “NM + WD” group, possibly suggesting that mycorrhizal induction could play a great role in plant 14-3-3 gene expression under the drought stress.…”

Section: Discussionmentioning

confidence: 92%

“…However, the exact function of the 14-3-3 protein in response to nutrient deficiency is unclear. At present, there are some studies on the role of tomato 14-3-3 gene family members under low P stress, mainly manifested in the aspects of starch accumulation and activation of plasma membrane H + -ATPase ( Xu et al, 2012a , b ; Zhang et al, 2018a ).…”

Section: Introductionmentioning

confidence: 99%

Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana

Han

Zhang

et al. 2022

Front. Microbiol.

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The application of arbuscular mycorrhizal fungi (AM fungi) and phosphorus (P) can improve plant growth under drought stress by upregulating the antioxidant system and osmotic accumulation. The 14-3-3 protein can respond to different abiotic stresses such as low P and drought. The purpose of this experiment was to study the effects of AM fungi (Rhizophagus intraradices) inoculation on reactive oxygen species (ROS) homeostasis, P metabolism, and 14-3-3 gene expression of Populus cathayana at different P levels and drought stress (WW: well-watered and WD: water deficit). Under WD conditions, AM fungi inoculation significantly increased the P content in leaves and roots, but the benefit in roots is limited by the level of P addition, and the roots may have more alkaline phosphatase and phytase under P stress, and these activities in the rhizosphere soil inoculated with AM fungi were stronger. Under WD conditions, the activities of catalase (leaf and root) and peroxidase (root) inoculated with AM fungi were significantly higher than those without inoculation and decreased with P addition. 14-3-3 genes, PcGRF10 and PcGRF11, have a positive correlation with the antioxidant system, osmotic regulation, and P metabolism, which may be more significant after inoculation with AM fungi. Our results provide new insights into the mechanism of ROS homeostasis and P metabolism in mycorrhizal plants under drought stress.

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“…(Cooper et al 2003;Roberts 2003;Chen et al 2006;Xu et al 2012b). In this study, we used a real-time RT-PCR in order to investigate the effect of As stress on the expression profile of 14-3-3 protein genes in the shoot of NCo and Co rice plants.…”

Section: Discussionmentioning

confidence: 99%

Arsenic stress affects the expression profile of genes of 14-3-3 proteins in the shoot of mycorrhiza colonized rice

Pathare

Srivastava

Sonawane

et al. 2016

Physiol Mol Biol Plants

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The intimate association between the arbuscular mycorrhizal fungi and host plants helps the latter in phosphate acquisition in exchange of carbohydrates and in enhanced stress tolerance. Similarly, the ubiquitous 14-3-3 protein family is known to be a major regulator of plant metabolism and stress responses. However, the involvement of mycorrhiza and plant 14-3-3 proteins interaction in plant response to environmental stimuli, such as arsenic (As) stress, is yet unknown. In this study, we analysed the impact of the As stress on the expression profile of 14-3-3 genes in the shoot of mycorrhiza colonized rice (Oryza sativa) plants. Ten day old rice seedlings were kept for 45 days for mycorrhizal colonisation (10 g inoculum per 120 g soilrite) and were then subjected to 12.5 lM arsenate [As(V)] exposure for 1 and 3 days, in hydroponics. Arsenate stress resulted in significant change in expression of 14-3-3 protein genes in non-colonized and mycorrhiza colonized rice plants which indicated As mediated effects on 14-3-3 proteins as well as interactive impact of mycorrhiza colonization. Indeed, mycorrhiza colonization itself induced up-regulation of all 14-3-3 genes in the absence of As stress. The results thus indicate that 14-3-3 proteins might be involved in As stress signalling and the mycorrhiza induced As stress response of the rice plants.

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Smart role of plant 14-3-3 proteins in response to phosphate deficiency

Cited by 9 publications

References 10 publications

Proteomics Analysis Reveals Non-Controlled Activation of Photosynthesis and Protein Synthesis in a Rice npp1 Mutant under High Temperature and Elevated CO2 Conditions

Proteomics Analysis Reveals Non-Controlled Activation of Photosynthesis and Protein Synthesis in a Rice npp1 Mutant under High Temperature and Elevated CO2 Conditions

Effect of arbuscular mycorrhizal fungi and phosphorus on drought-induced oxidative stress and 14-3-3 proteins gene expression of Populus cathayana

Arsenic stress affects the expression profile of genes of 14-3-3 proteins in the shoot of mycorrhiza colonized rice

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