<i>Streptococcus bovis</i> septic shock due to contaminated transfused platelets

Chang, Andrew; Kirsch, Carl M.; Mobashery, Niloufar; Johnson, Nancy; Levitt, Lee J.

doi:10.1002/ajh.20168

Cited by 13 publications

(18 citation statements)

References 26 publications

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“…Five hundred and five non-contaminated bags were administered to 135 patients (median 3; range: [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. In the group of 17 patients receiving contaminated PBPCs, a total of 71 bags were administered (the 31 contaminated ones referred to above, plus 40 uncontaminated bags) (median 5; range: 1-9); a median of 1 contaminated bag per patient (range 1-5).…”

Section: Resultsmentioning

confidence: 99%

“…This phenomenon has been reported by other authors 6,13 although no explanation is given in these reports. It is possible that storing the product in liquid nitrogen and DMSO decreases the bacterial burden 4,22 and this makes it impossible to detect bacteria present pre-freezing, whereas a new contamination occurs during the thawing process.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3][4] Microbial contamination has also been reported in blood processed for immunotherapy 5 and in hematopoietic progenitor cell transplants. In these cases, the contamination may be, according to the source of stem cells, from 0 to 4.5% in peripheral blood to as high as 26% in bone marrow.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant

Majado

García-Hernández

Morales

et al. 2006

Bone Marrow Transplant

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant

Majado

García-Hernández

Morales

et al. 2006

Bone Marrow Transplant

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“…Há vários casos relatados associando sepse após infusão plaquetária 1,18,19,[21][22][23][24] . Uma reação transfusional é caracterizada através de sinais e sintomas tais como: febre (com aumento de temperatura de 1 a 2°C), calafrios, tremores, hipotensão, ruborização, náusea, vômitos e choque 25 .…”

Section: Métodos Resultadosunclassified

Contaminação bacteriana em concentrados plaquetários: identificação, perfil de sensibilidade aos antimicrobianos e sepse associada à transfusão

Martini

Kempfer

Rodrigues

et al. 2010

Rev. Soc. Bras. Med. Trop.

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RESUMOIntrodução: Devido à sepse bacteriana associada à transfusão de concentrados plaquetários (CPs) ter sérias consequências clínicas para os pacientes, alguns procedimentos têm sido incorporados na preparação e no controle de qualidade dos componentes sanguíneos para reduzir o risco da contaminação bacteriana. Este artigo descreve a prevalência da contaminação bacteriana dos CPs que foram transfundidos, o espectro bacteriano detectado com seu perfil de sensibilidade aos antimicrobianos e as reações transfusionais nos receptores. Métodos: Um total de 292 CPs (278 randômicos e 14 por aférese), proveniente do Hemocentro do Estado do Rio Grande do Sul (HEMORGS) de Santa Maria foi testado. As quantidades de 100µL e 200µL foram coletadas da porção tubular da bolsa de plaquetas e semeadas utilizando dois tipos de metodologias. Resultados: Em cinco unidades(1,7%; 5/292) foram isoladas bactérias pela metodologia qualitativa e apenas uma pela quantitativa. Staphylococcus epidermidis foi o microrganismo identificado em todas as amostras. Dois pacientes apresentaram sepse associada à transfusão com desfecho fatal. Conclusões: A contaminação bacteriana pelas transfusões de CPs constitui-se num importante problema de saúde pública devido a sua associação com altas taxas de morbidade e mortalidade. Neste estudo, somente microrganismos gram-positivos foram isolados sendo que nenhuma amostra obtida por aférese apresentou contaminação. Palavras-chaves: Concentrados plaquetários. Contaminação bacteriana. Sepse. Staphylococcus epidermidis. ABSTRACTIntroduction: Bacterial sepsis associated with the transfusion of platelet concentrates (PCs) results in serious clinical implications for patients. Given these implications, certain procedures have been integrated into the preparation and quality control of blood components to reduce the risk of bacterial contamination. This article describes the prevalence of bacterial contamination on transfused PCs, the bacterial spectrum detected and their antimicrobial susceptibility profile and transfusion reactions in receptors. Methods: A total of 292 PCs (278 random and 14 per apheresis) from the Blood Center of the State of Rio Grande do Sul (HEMORGS), located in the city of Santa Maria, were tested. Quantities of 100µL and 200µL were collected from platelet bag tubing and seeded using two methodologies. Results: Using the qualitative methodology, bacteria were isolated in five units (1.7%; 5/292), while only one was isolated using the quantitative methodology. Staphylococcus epidermidis was the microorganism identified in all samples. Two patients died of transfusion-related sepsis. Conclusions: Bacterial contamination due to PC transfusion is considered a major public health problem due to its association with high rates of morbidity and mortality. In this study only gram-positive microorganisms were isolated and none of the samples obtained by apheresis presented contamination.

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“…PCs are blood components with the highest bacterial contamination rate and usually responsible for most of septic transfusion reactions (Chang et al, 2004). This type of bacterial contamination due to PCs infusion is much higher than other types of transfusion-transmitted infections (Jacobs, Palavecino, Yomtovian, 2001;Goodnough, Sander, Brecher, 2003;Vasconcelos et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial susceptibility profile and research of mec A and erm genes in coagulase-negative staphylococci isolated from platelet concentrates bags

Martini

Hörner

Graichen³

2017

Braz. J. Pharm. Sci.

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In recent years, several studies have described the clinical impact of bacterial infection associated with transfusion of platelet concentrates (PCs). Among the blood components, PCs are responsible for the highest rates of bacterial contamination as well as septic transfusion reactions. We assessed antimicrobial susceptibility profile, resistance to methicillin (MRCoNS), and resistance to macrolides, lincosamides and streptogramins of group B (MLS B ) of 16 coagulase-negative staphylococci (CoNS) isolates from an investigation in 691 PCs bags. We then compared conventional and automated phenotypic methods, disc diffusion test (DD) and VITEK® 2, respectively as well as phenotypic and genotypic methods (Polymerase Chain Reaction -PCR). All CoNS were susceptible to vancomycin. The disc diffusion test characterized 18.75% as MRCoNS and 37.5% with inducible resistance to MLS B (iMLS B ), and with VITEK® 2, 6.3% and 31.25%, respectively. The mecA gene was detected in 18.75% and the erm gene in 31.25% of the isolates. In this study, we found equal percentage values between presence of the mecA gene by PCR and resistance to methicillin using cefoxitin by DD test, evidence of the erm gene by PCR, and iMLS B resistance by automation (VITEK® 2). Moreover, we identified three strains with beta-lactamase overproduction, and the occurrence of a bigger mistake was verified when automation was compared with DD test. And we observed that D-test was the most reliable for the detection of iMLS B resistance in Staphylococcus sp.

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Streptococcus bovis septic shock due to contaminated transfused platelets

Cited by 13 publications

References 26 publications

Influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant

Influence of harvest bacterial contamination on autologous peripheral blood progenitor cells post-transplant

Contaminação bacteriana em concentrados plaquetários: identificação, perfil de sensibilidade aos antimicrobianos e sepse associada à transfusão

Antimicrobial susceptibility profile and research of mec A and erm genes in coagulase-negative staphylococci isolated from platelet concentrates bags

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