<i>Staphylococcus pseudintermedius</i> biofilms secrete factors that induce inflammatory reactions <i>in vitro</i>

Arima, Sayuri; Ochi, Hiroki; Mitsuhashi, Masanobu; Kibe, Ryoko; Takahashi, Koji; Kataoka, Yuki

doi:10.1111/lam.13018

Cited by 18 publications

(16 citation statements)

References 26 publications

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“…In this sense, the differential modulation of proinflammatory cytokines such as il-1β , il-12 , il-6 , and tnf-α were previously described in mice purified peritoneal macrophages treated with eight different isolates of sessile P. aeruginosa [ 70 ]. In the case of Staphylococcus pseudintermedius , RAW 264.7 cells macrophages treated with biofilm purified from this bacterium induced high responses of il-1β and il-6 , which was attributed to the interaction of the cells with biofilm protein components, activating the signaling pathway of Toll and NF-kB receptors [ 71 ]. These results demonstrate that the interaction of the sessile form of LF-89 with SHK-1 cells was different from that obtained with the planktonic form, probably due to specific components in its exopolysaccharide matrix, which could increase the expression of the evaluated immune genes, concomitant to increased cytotoxicity of this strain in the cell line.…”

Section: Discussionmentioning

confidence: 99%

Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1

et al. 2020

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Piscirickettsia salmonis is the causative agent of Piscirickettsiosis, an infectious disease with a high economic impact on the Chilean salmonid aquaculture industry. This bacterium produces biofilm as a potential resistance and persistence strategy against stressful environmental stimuli. However, the in vitro culture conditions that modulate biofilm formation as well as the effect of sessile bacteria on virulence and immune gene expression in host cells have not been described for P. salmonis. Therefore, this study aimed to analyze the biofilm formation by P. salmonis isolates under several NaCl and iron concentrations and to evaluate the virulence of planktonic and sessile bacteria, together with the immune gene expression induced by these bacterial conditions in an Atlantic salmon macrophage cell line. Our results showed that NaCl and Fe significantly increased biofilm production in the LF-89 type strain and EM-90-like isolates. Additionally, the planktonic EM-90 isolate and sessile LF-89 generated the highest virulence levels, associated with differential expression of il-1β, il-8, nf-κb, and iκb-α genes in SHK-1 cells. These results suggest that there is no single virulence pattern or gene expression profile induced by the planktonic or sessile condition of P. salmonis, which are dependent on each strain and bacterial condition used.

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Section: Discussionmentioning

confidence: 99%

Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1

et al. 2020

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“…The ability of S. pseudintermedius to produce biofilms has been previously reported. In Japan, all S. pseudintermedius isolates were able to produce biofilm [ 17 ], while in South Korea, a biofilm-forming quantitative assay showed that all S. pseudintermedius isolates produced either strong or moderate biofilm [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Profile and Biofilm Production of Staphylococcus pseudintermedius Isolated from Dogs in Thailand

et al. 2021

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Staphylococcus pseudintermedius is a zoonotic pathogen that can cause life-threatening infections in animals and humans. The study of methicillin-resistant S. pseudintermedius (MRSP) and its ability to produce biofilms is important to select the most suitable treatment. The prevalence and characteristics of S. pseudintermedius isolated from dogs admitted at the Veterinary Teaching Hospital, Prince of Songkla University, Thailand were assessed. Results showed that 28.30% (15/53) of the isolates were MRSP. Amplification of the mecA gene was observed in 93.33% (14/15) MRSP. Methicillin-resistant strains revealed co-resistant patterns against other antibiotics, including chloramphenicol, clindamycin, tetracycline, clarithromycin, ciprofloxacin, and trimethoprim. In this study, all bacterial isolates produced biofilms, while 90.55% of S. pseudintermedius isolates were strong or moderate biofilm producers. Most (45–60%) of the resistant strains were strong biofilm producers, while the correlation between biofilm production and antibiotic resistance was not statistically significant. This is the first study in southern Thailand to investigate the drug-resistant profile of S. pseudintermedius and its ability to form biofilm. The results will contribute to a better understanding of the emergence and prevalence of antimicrobial resistance in S. pseudintermedius.

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“…Biofilm formation of Staphylococcus spp. including S. pseudintermedius is well known as a factor which increases the severity of diseases [7]. Biofilms are groups of bacteria which are enclosed by the extracellular polymeric substances that help bacteria to survive in inappropriate environments longer than planktonic cells, which leads to decrease in antibiotic susceptibility [8,9].…”

Section: Introductionmentioning

confidence: 99%

Anti-biofilm activity of Thai herbal essential oils against Staphylococcus pseudintermedius causing canine pyoderma

Aiemsaard¹,

Kamollert²,

Singh³

et al. 2020

ScienceAsia

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The aim of this study was to investigate the efficacy of 11 essential oils (EOs) against Staphylococcus pseudintermedius planktonic and biofilm forms. Twenty three bacterial samples were collected from dogs with superficial pyoderma and 9 samples were identified as S. pseudintermedius by polymerase chain reaction-restriction fragment length polymorphism method. The minimum planktonic inhibitory concentration (MPIC), minimum planktonic bactericidal concentration (MPBC) and minimum biofilm eradication concentration (MBEC) of EOs were determined by broth microdilution using a transferable solid phase. Among all, lemongrass EO was the most effective against both planktonic and biofilm forms of S. pseudintermedius with the MPIC, MPBC and MBEC of 0.02, 0.039 and 0.078% v/v, respectively. EOs of betel vine and citronella had the second most inhibitory effect (MPIC and MPBC: 0.039% v/v and MPBC: 0.156% v/v) followed by clove (MPIC and MPBC: 0.078% v/v) and sweet basil (MPIC and MPBC: 0.625% v/v). The EOs of ginger, plai, kaffir lime, turmeric, holy basil and galanga had the lowest inhibitory effect with MPICs and MPBCs ranging from 1.25% v/v to greater than 2.5% v/v. The results of this study indicated that the anti-planktonic and anti-biofilm effects of the tested EOs were concentration dependent, and higher concentrations were required for anti-biofilm activity. Lemongrass EO showed the most potential as a therapeutic antimicrobial agent for both planktonic and biofilm forms of S. pseudintermedius.

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Staphylococcus pseudintermedius biofilms secrete factors that induce inflammatory reactions in vitro

Cited by 18 publications

References 26 publications

Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1

Biofilm Produced In Vitro by Piscirickettsia salmonis Generates Differential Cytotoxicity Levels and Expression Patterns of Immune Genes in the Atlantic Salmon Cell Line SHK-1

Antibiotic Resistance Profile and Biofilm Production of Staphylococcus pseudintermedius Isolated from Dogs in Thailand

Anti-biofilm activity of Thai herbal essential oils against Staphylococcus pseudintermedius causing canine pyoderma

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