<i>Salvia Fruticosa, Salvia Officinalis,</i>and Rosmarinic Acid Induce Apoptosis and Inhibit Proliferation of Human Colorectal Cell Lines: The Role in MAPK/ERK Pathway

Xavier, Cristina P.R.; Lima, Cristóvão F.; Fernandes-Ferreira, Manuel; Pereira‐Wilson, Cristina

doi:10.1080/01635580802710733

Cited by 150 publications

(106 citation statements)

References 38 publications

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“…For example, Xavier et al showed that RA reduced cell growth and inhibited the MAPK/ERK pathway in the colon carcinoma-derived cell line, CO115 [46]. Consistent with this finding, we observed inhibition of ERK phosphorylation in HNSCC following treatment with 80 μg/ml RA ( Figure 2C).…”

Section: Discussionsupporting

confidence: 80%

Rosmarinic Acid Inhibits Cell Growth and Migration in Head and Neck Squamous Cell Carcinoma Cell Lines by Attenuating Epidermal Growth Factor Receptor Signaling

Tumur¹,

Guerra²,

Yanni³

et al. 2015

J Cancer Sci Ther

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Objective: Active components of natural foods are increasingly receiving attention for their chemopreventive and chemotherapeutic potential in a wide variety of cancers. Rosmarinic acid (RA), a phenolic compound in various herbal plants, is well-recognized for its anti-oxidant, anti-inflammatory, anti-proliferative properties in a variety of cell types. In this report, we describe a novel role for RA as an inhibitor of epidermal growth factor (EGF) stimulated signaling in HNSCC and propose a cellular reactive oxygen species (ROS)-mediated mechanism for the observed effects.Methods: Cellular growth, migration and reactive oxygen species (ROS) profiles were examined in RA-treated and untreated HNSCC cell lines (UM-SCC-6 and UM-SCC-10B) using the WST-1 viability assay, established in vitro migration assays, and the CM-H2DCFDA ROS assay, respectively. The influence of RA on EGF-stimulated phosphorylation and its downstream pathways was evaluated using Western-blotting techniques.Results: RA inhibited cell viability, migration and cellular production of ROS in HNSCC cell lines. Furthermore, RA inhibited EGF-induced phosphorylation of the EGFR at tyrosine residues 992 and 845, which led to downregulation of the phosphatidylinositol 3-kinase Akt (PI3K/Akt) and mitogen-activated protein kinase ERK (MAPK/ ERK) pathways.Conclusions: This is the first report describing both growth-and motility-inhibitory roles for RA in HNSCC cells. Additionally, our study is the first to demonstrate that treatment with RA can reduce EGF-induced activation of PI3K/ Akt in tumor cells. The present data suggests that RA holds promise as a chemotherapeutic agent against HNSCC. a novel strategy to down-regulate EGFR signaling using the phenolic compound, rosmarinic acid. RosmarinicActive components of natural foods are increasingly receiving attention for their chemopreventive and chemotherapeutic potential in a wide variety of cancers. One such food component, rosmarinic acid (RA), is a widely distributed phenolic compound in various herbal plants such as rosemary, sweet basil, sage, mint, and perilla (Figure 1) [10,11]. RA is regarded as a daily-consumed safe ingredient, due to its extensive use in the food industry for flavoring, but studies have also elucidated a medicinal role for RA, especially in regard to its anti-oxidant, and anti-inflammatory activities [12,13]. Furthermore, an emerging body of literature has also described the growth inhibitory and anti-invasion properties of RA in colon, skin, breast and ovarian cancers in vitro and/or in vivo [14][15][16][17][18][19], but the mechanisms underlying these effects are poorly understood. A chemopreventive role for RA in HNSCC has been described in 7,12-dimethylbenz (a) anthracene-induced hamster buccal pouch carcinogenesis [20], but a chemotherapeutic role for RA in the treatment of HNSCC has not yet been reported.Given the anti-oxidant properties attributed to RA, it is possible that RA's anti-tumor effects stem from its ability to combat oxidative stress in HNSCC. Oxidative ...

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Section: Discussionsupporting

confidence: 80%

Rosmarinic Acid Inhibits Cell Growth and Migration in Head and Neck Squamous Cell Carcinoma Cell Lines by Attenuating Epidermal Growth Factor Receptor Signaling

Tumur¹,

Guerra²,

Yanni³

et al. 2015

J Cancer Sci Ther

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“…medicine, but the cytotoxicity of S. officinalis has been previously reported (Xavier et al 2009;El Hadri et al 2010). An essential oil prepared by sub-fractionation of S.…”

Section: S Officinalis Is Not Currently Used For Anticancer Treatmenmentioning

confidence: 97%

The influence of extraction solvents on the anticancer activities of Palestinian medicinal plants

Alzeer¹,

Vummidi²,

Arafeh³

et al. 2014

J. Med. Plants Res.

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Palestine has a rich and prestigious heritage of herbal medicines. To investigate the impact of variable extraction techniques on the cytotoxic effects of medicinal plant extracts, 5 well-known medicinal plants from Palestine were extracted with 90% ethanol, 80% methanol, acetone, coconut water, apple vinegar, grape vinegar or 5% acetic acid. The resulting 35 extracts were screened for cytotoxic activities against three different cancer cell lines (B16F10, MCF-7 and HeLa) using a standard resazurin-based cytotoxicity assay and Nile Blue A as the positive control. Highly variable toxicities and tissue sensitivity were observed, depending upon the solvent used for extraction. The acetone extract of Salvia officinalis L. exhibited the most potent cytotoxicity (IC50 = 14 -36 ug/ml), but very little sensitivity between the three cell lines. More moderate cytotoxicity with improved tissue sensitivity was observed with coconut water extract of Salvia officinalis L (IC50 = 114 µg/ml) and methanolic extract of Teucrium polium L (IC50 = 104 µg/ml). In this study, acetone consistently gave lower extraction yields but higher cytotoxicity, whereas other solvent systems gave much higher extraction yields with lower cytotoxicity. These results demonstrate how the cytotoxicity of plant extracts can be inversely proportional to the yield, and that solvent selection plays an important role in both factors. (IC 50 = 104 µg/ml). In this study, acetone consistently gave lower extraction yields but higher cytotoxicity, whereas other solvent systems gave much higher extraction yields with lower cytotoxicity. These results demonstrate how the cytotoxicity of plant extracts can be inversely proportional to the yield, and that solvent selection plays an important role in both factors.

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“…Antioxidant phytochemicals, through their ROS scavenging activity, may suppress altered redox-sensitive signalling events in cancer. Xavier et al [9] investigated the antiproliferative and proapoptotic effects of S. officinalis, S. fruticosa and rosmarinic acid on human colon carcinoma cell lines, HCT15 and CO115, which have different mutations in MAPK/ERK and phosphatidylinositol 3-kinase (PI3K/Akt) signalling pathways. Interestingly, they found that SF, SO and rosmarinic acid induce apoptosis in both cell lines, whereas cell proliferation was inhibited by the two sage extracts only in HCT15.…”

Section: Salvia Officinalis Salvia Officinalis L (Common Sagementioning

confidence: 99%

“…This plant of the family Lamiaceae, native to the Mediterranean region, is known for its antibacterial, antiinflammatory, antifungal, antioxidant and antiproliferative activities [6][7][8][9].…”

Section: Salvia Officinalis Salvia Officinalis L (Common Sagementioning

confidence: 99%

Biological effects of four frequently used medicinal plants of Lamiaceae

Srančíková¹,

Horvathova²,

Kozics³

2014

neo

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Cancer is one of the leading causes of death characterized by uncontrolled growth and spread of cancer cells. There are several hundred thousands of new cases of cancer worldwide. Clinical oncology is still challenged by toxicity and side effects of multimodal therapy strategies in which it is associated with poor prognosis for patients. There is an urgent necessity to develop novel therapy strategies and to utilize preventive potential of natural compounds. As the majority of anticancer drugs are of natural origin, natural products represent a valuable source for the identification and development of novel treatment options and chemopreventive mechanisms for cancer. This review is focused on the summary of published knowledges on the antioxidant and potential chemopreventive effects of biologically active substances present in the extracts of four plants of the family Lamiaceae (sage, thyme, rosemary and lavander) in different animal and in vitro systems. It is assumed that the chemopreventive and chemotherapeutic potential of natural compounds is the result of a combined action of several mechanisms.

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Salvia Fruticosa, Salvia Officinalis,and Rosmarinic Acid Induce Apoptosis and Inhibit Proliferation of Human Colorectal Cell Lines: The Role in MAPK/ERK Pathway

Cited by 150 publications

References 38 publications

Rosmarinic Acid Inhibits Cell Growth and Migration in Head and Neck Squamous Cell Carcinoma Cell Lines by Attenuating Epidermal Growth Factor Receptor Signaling

Rosmarinic Acid Inhibits Cell Growth and Migration in Head and Neck Squamous Cell Carcinoma Cell Lines by Attenuating Epidermal Growth Factor Receptor Signaling

The influence of extraction solvents on the anticancer activities of Palestinian medicinal plants

Biological effects of four frequently used medicinal plants of Lamiaceae

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