Retracted: Sapylin inhibits lung cancer cell proliferation and promotes apoptosis by attenuating PI3K/AKT signaling

Abstract

Sapylin (OK‐432) revealed biological properties in cancers. In this study, the effect of sapylin on lung cancer cell A549 was investigated. A549 cell lines were treated with sapylin (0.1, 0.5, and 1 KE/mL) for different time intervals. A549 cell proliferation and apoptosis was determined using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide/Ki67 assay and flow cytometry, respectively. Western blot was used to determine the expressions of proteins involved in proliferation, apoptosis, and phosphoinositide 3‐kinase/serine/threonine kinase (PI3K/AKT), Wnt3a/β‐catenin signaling pathway. Level of intracellular reactive oxygen species (ROS) was insured by using the ROS kit. Sapylin inhibited A549 cell viability and the expressions of proliferation‐related proteins (cyclin E1 and D1) in dose‐ and time‐dependent manners. Sapylin promoted apoptosis in a dose‐ and time‐dependent manners. Sapylin also promoted the expressions of apoptotic proteins (cleaved caspase‐3 and 8) in dose‐ and time‐dependent manners. Furthermore, sapylin increased the intracellular concentration of ROS in a dose‐dependent manner. Besides, the high expression of ROS level might induce inhibition of cell viability and increase cell apoptosis. The mechanistic study revealed that sapylin inactivated the PI3K/AKT and Wnt3a/β‐catenin signaling pathways. Our findings suggest that sapylin inhibits proliferation and promotes apoptosis in lung cancer cells, thus providing a new theoretical basis for the treatment of lung cancer.

Anti-Lung Cancer Targets of Radix Paeoniae Rubra and Biological Molecular Mechanism: Network Pharmacological Analyses and Experimental Validation

“… 39 , 40 Zhang et al found that reducing the phosphorylation of PI3K and AKT and restraining activation of PI3K-AKT pathway could restrain the proliferation and promote the apoptosis of LC cells. 41 …”

“…39,40 Zhang et al found that reducing the phosphorylation of PI3K and AKT and restraining activation of PI3K-AKT pathway could restrain the proliferation and promote the apoptosis of LC cells. 41 In vitro, we selected baicalein, paeoniflorin, ellagic acid, (+)-catechin of the active ingredients in RPR to observe the inhibitory effect on A549 cells. Paeoniflorin has been proven with anti-tumor effects in nasopharyngeal carcinoma cells via downregulation of NEDD4.…”

PIK3R1 Promotes Lung Cancer Proliferation Through Activating PI3K/AKT/mTOR Signaling Pathways

“…Jiang M et al studied the regulation of a sa ower chemical compound extraction, hydroxysa or yellow A (HSYA), and found it worked as a potential candidate in treatment of NSCLC via targeting PI3K/AKT/mTOR signaling pathways [36]. Sapylin (OK-432) suppressed lung cancer cell proliferation and promoted apoptosis through inhibiting PI3K/AKT signaling, providing a new theoretical basis for lung cancer treatment [37]. Wang J et al synthesized a novel compounds of PI3K/AKT pathway inhibitors W934 for lung cancer [38].…”