<i>RETRACTED:</i> Role of mesenchymal stem cells versus angiotensin converting enzyme inhibitor in kidney repair

Ahmed, Hanaa H.; Toson, El-Shahat A.; El-mezayen, Hatem A.; Rashed, Laila Ahmed; Elsherbiny, Eslam Samy

doi:10.1111/nep.12812

Cited by 11 publications

(6 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Paraffin blocks were sectioned at 4 microns using a rotary microtome, distributed onto glass slides, and then dried overnight. The attained tissue sections were gathered on glass slides deparaffinized and exposed to hematoxylin and eosin (H&E) stains (Servicebio Technology Co., Ltd.) for histopathological examination through the electric light microscope (Ahmed et al., 2017; Toson et al., 2019).…”

Section: Methodsmentioning

confidence: 99%

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

2021

View full text Add to dashboard Cite

The habit of drinking tea is highly prevalent and could be utilized to introduce more health benefits through fortification with medicinal plants. The purpose of this analysis was to assess the nutritional quality and health benefits of fortified Ziziphus tea (ZT) with green tea (GT) against obesity and non‐alcoholic fatty liver disease (NAFLD). Proximate analysis and sensory evaluation were carried out on the fortified tea. In the in vivo study, 15 SD rats were used for each group. Flow cytometry was utilized for caspase 3 analysis. ELISA was used for the detection of tumor necrosis factor‐alpha and adiponectin levels. Real‐time PCR was used to detect Notch‐1 and Hes‐1 gene expression. The composition of fortified (GT+ZT) showed a significant improvement in the nutritional value represented by the increase in overall protein, crude fat, crude fiber, ash, carbohydrate, mineral contents, and antioxidant capacity. Treatment with GT+ZT restored the disturbance in body weight, lipid profile, liver function, glucose, insulin sensitivity index, and oxidative status. It reversed the changes in TNF‐α and adiponectin levels. Their protective effects against NAFLD were indicated by the inhibition of hepatic caspase‐3 activity, suppression of Notch‐1, and Hes‐1 gene expression and amelioration of high‐fat diet (HFD)‐induced histological alterations. Collectively, our findings, elucidate the precise mechanism of fortified ZT+GT for the attenuation of obesity‐induced metabolic disorders and NAFLD via regulating lipolysis, TNF‐α, adiponectin, apoptosis, and Notch‐1 signaling pathways, and provide a foundation for an easily implemented healthy habit of drinking. Practical applications The incorporation of bioactive compounds into functional foods is a growing market. Consumer attention in well‐being has increased rapidly toward a fortified diet that provides additional health effects. The fortified (GT+ZT) tea may potentially serve as an easily implemented healthy drinking habit to prevent and manage obesity and NAFLD and reduce the risk of other diseases. Fortification with ZT improved the health‐promoting functionality of GT through the enhancement of total protein, carbohydrates, antioxidant, and mineral contents. This was reflected by their synergetic therapeutic activity in ameliorating the disturbance in obesity‐related disorders and NAFLD via regulating lipolysis, inflammation, oxidative stress, apoptosis, and Notch‐1 signaling pathways. Therefore, (GT+ZT) could be considered functional foods which attribute to functional improvement and reduction in disease risk.

show abstract

Section: Methodsmentioning

confidence: 99%

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

2021

View full text Add to dashboard Cite

show abstract

“…The immune‐enhancing activity of SL and FS ameliorated the CSA‐induced inflammatory effects through reducing the elevated levels of transforming growth factor (TGF‐β1). TGF‐β1 is a pro‐inflammatory protein that facilitates renal fibrosis by triggering its downstream proteins and increasing production and reducing the destruction of extracellular matrix (ECM) components in kidney (Ahmed et al., 2017; Sureshbabu et al., 2016). In the current study, elevated levels IFN‐γ induced after treatment with SL and FS mediate the suppression of TGF‐β/Smad signaling pathway leading to reduction of TGF‐β1 levels (Kimura et al., 2006), suggesting their anti‐inflammatory activity.…”

Section: Discussionmentioning

confidence: 99%

“…Paraffin bees wax tissue blocks were prepared for sectioning at 4 microns by microtome. The obtained tissue sections were collected on glass slides, deparaffinized and stained by hematoxylin and eosin (H&E) stains for histopathological examination through the electric light microscope (Ahmed et al., 2017).…”

Section: Methodsmentioning

confidence: 99%

“…CsA (Novartis Pharma, Basel, Switzerland) was emulsified in olive oil (Sigma Chemical, Diagnostics, St Louis, MO, USA), and it was taken orally with 20 mg/Kg/day for 2 weeks (Ahmed et al, 2017;Elsayed et al, 2016Elsayed et al, , 2018. Treatment with either SL, FS, or (SL + FS) was applied daily for 1 month mixed with the standard rodent diet introduced.…”

Section: In Vivo Investigations Of the Potentialmentioning

confidence: 99%

“…Cyclosporine A (CsA), a potent immunosuppressant, is the most commonly used medications in organ transplantation and in the treatment of many autoimmune diseases (Ahmed et al., 2017). Cyclosporine A works by modulating the generation of cytokines involved in the immune system, including interleukins 1a & 1b, interleukin 6, π‐interferon, and other lymphokines 8,14 stimulating hematopoiesis, modulating inflammatory and immune responses, and manipulating immunity (Leas et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A

2021

View full text Add to dashboard Cite

The focus of consumers in healthy food turned to the possible health benefits of particular foods and food ingredients. This study aimed to evaluate the newly fortified biscuits supplemented with sidr leaves and flaxseed and to highlight their nutritional quality and health benefits against cyclosporine A-induced dexterous effects.Sidr leaves (SL), and flaxseed (FS) were used in the preparation of fortified biscuits.Proximate analysis and sensory evaluation were carried out on the biscuits. In in vivo study, 15 male albino mice were used for each group. Groups were divided into control, CsA, SL, FS, and SL+FS-treated groups. Hematological analysis, kidney function tests, oxidative stress, and anti-oxidant status were estimated. Flow cytometry was utilized to detect apoptosis and autophagy levels. The enzyme-linked immunosorbent assay (ELISA) was used for detection of interleukin-2 (IL-2), interferonγ (IFNγ), and transforming growth factor β1 (TGF-β1) levels. The composition of biscuits complemented by SL and FS demonstrated significant improvement in the nutritional value represented by the increase in overall protein, crude fat, crude fiber, ash, and carbohydrate contents. Treatment with SL and FS restored the disturbance in hematological, kidney function, oxidative, and antioxidant biomarkers. CsA-induced apoptotic and autophagic renal cell death was suppressed. Cytokines and pro-inflammatory markers were ameliorated. The use of SL and FS in dietary products can be recommended as a functional food. Moreover, they showed renal-protective, antioxidant, anti-inflammatory, and immune-enhancing activities. Practical applicationsSidr leaves (SL) and flaxseed (FS) were used in the preparation of fortified biscuits.The composition of biscuits complemented by SL and FS demonstrated a significant improvement in the nutritional values represented by the increase in overall protein, crude fat, crude fiber, ash, and carbohydrate contents. SL and FS showed a potential therapeutic activity in reversing CsA-induced dexterous side effects by acting as an antioxidant, antiapoptotic, antiautophagic, anti-inflammatory, renal-protective, and How to cite this article: El Seedy GM, El-Shafey ES, Elsherbiny ES. Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A.

show abstract

The role of apoptosis and autophagy in the insulin-enhancing activity of oxovanadium(IV) bipyridine complex in streptozotocin-induced diabetic mice

El‐Shafey

Elsherbiny

2020

Biometals

View full text Add to dashboard Cite

RETRACTED: Role of mesenchymal stem cells versus angiotensin converting enzyme inhibitor in kidney repair

Abstract: The current data provided distinct evidence about the favourable impact of AD-MSCs and BM-MSCs in attenuation of cyclosporine-induced nephropathy in rats through their ability to promote functional and structural kidney repair via transdifferentiation.

Cited by 11 publications

References 52 publications

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A

The role of apoptosis and autophagy in the insulin-enhancing activity of oxovanadium(IV) bipyridine complex in streptozotocin-induced diabetic mice

Contact Info

Product

Resources

About