The role of apoptosis and autophagy in the insulin-enhancing activity of oxovanadium(IV) bipyridine complex in streptozotocin-induced diabetic mice

El‐Shafey, Eman Salah; Elsherbiny, Eslam Samy

doi:10.1007/s10534-020-00237-1

Cited by 19 publications

(15 citation statements)

References 39 publications

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“…Consequently, it has been shown that inflammation and oxidative stress, which pathologically contribute to obesity, play a significant role in developing insulin resistance (El‐Shafey & Elsherbiny, 2020). In the present study, the HDF group showed an increase in oxidative stress marker and a significant reduction in the activity of antioxidant enzymes relative to the control group.…”

Section: Discussionmentioning

confidence: 99%

“…The PH was adjusted at 7.5 by 1N HCl and then suspended cells were applied to centrifugation at 280 g for 10 mints and aspiration of the supernatant was performed. Cells were fixed in ice‐cold 96%–100% ethanol (BDH) in around 1 ml within each cell, preserved in a refrigerator, and can also be mailed without running a sample (El‐Shafey & Elsherbiny, 2020).…”

Section: Methodsmentioning

confidence: 99%

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Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

2021

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The habit of drinking tea is highly prevalent and could be utilized to introduce more health benefits through fortification with medicinal plants. The purpose of this analysis was to assess the nutritional quality and health benefits of fortified Ziziphus tea (ZT) with green tea (GT) against obesity and non‐alcoholic fatty liver disease (NAFLD). Proximate analysis and sensory evaluation were carried out on the fortified tea. In the in vivo study, 15 SD rats were used for each group. Flow cytometry was utilized for caspase 3 analysis. ELISA was used for the detection of tumor necrosis factor‐alpha and adiponectin levels. Real‐time PCR was used to detect Notch‐1 and Hes‐1 gene expression. The composition of fortified (GT+ZT) showed a significant improvement in the nutritional value represented by the increase in overall protein, crude fat, crude fiber, ash, carbohydrate, mineral contents, and antioxidant capacity. Treatment with GT+ZT restored the disturbance in body weight, lipid profile, liver function, glucose, insulin sensitivity index, and oxidative status. It reversed the changes in TNF‐α and adiponectin levels. Their protective effects against NAFLD were indicated by the inhibition of hepatic caspase‐3 activity, suppression of Notch‐1, and Hes‐1 gene expression and amelioration of high‐fat diet (HFD)‐induced histological alterations. Collectively, our findings, elucidate the precise mechanism of fortified ZT+GT for the attenuation of obesity‐induced metabolic disorders and NAFLD via regulating lipolysis, TNF‐α, adiponectin, apoptosis, and Notch‐1 signaling pathways, and provide a foundation for an easily implemented healthy habit of drinking. Practical applications The incorporation of bioactive compounds into functional foods is a growing market. Consumer attention in well‐being has increased rapidly toward a fortified diet that provides additional health effects. The fortified (GT+ZT) tea may potentially serve as an easily implemented healthy drinking habit to prevent and manage obesity and NAFLD and reduce the risk of other diseases. Fortification with ZT improved the health‐promoting functionality of GT through the enhancement of total protein, carbohydrates, antioxidant, and mineral contents. This was reflected by their synergetic therapeutic activity in ameliorating the disturbance in obesity‐related disorders and NAFLD via regulating lipolysis, inflammation, oxidative stress, apoptosis, and Notch‐1 signaling pathways. Therefore, (GT+ZT) could be considered functional foods which attribute to functional improvement and reduction in disease risk.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

2021

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“…Repressing the proliferation and promoting apoptosis of tumour cells are promising methods in inhibiting the development of tumours. [19][20][21] On the other hand, evidence has shown that tumour cell migration and invasion lead to cancer metastasis. 22,23 In addition, previous studies have demonstrated that the use of inhaled anaesthetics SEV and isoflurane exerts tumour-suppressive effects in human cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…Repressing the proliferation and promoting apoptosis of tumour cells are promising methods in inhibiting the development of tumours 19–21 . On the other hand, evidence has shown that tumour cell migration and invasion lead to cancer metastasis 22,23 .…”

Section: Discussionmentioning

confidence: 99%

Sevoflurane inhibits neuroblastoma cell proliferation and invasion and induces apoptosis by miR‐144‐3p/YAP1 axis

Yang

Zhang

et al. 2021

Basic Clin Pharma Tox

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Background: Sevoflurane (SEV) is a typical volatile anaesthetic and has an antitumour activity in various cancer cells. Here, we were curious whether SEV has tumour-suppressive effects in neuroblastoma (NB).Methods: NB cell lines (K-N-SH and SK-N-AS) were treated with SEV (1%, 2% and 4%). Cell Counting Kit-8 (CCK8) and Transwell assays were conducted to examine cell proliferation and invasion, respectively. The apoptosis was verified by flow cytometry, and the yes-associated protein 1 (YAP1), Bax, Bcl2 and cleaved caspase3 levels were detected by western blotting. Quantitative real-time PCR (qRT-PCR) was conducted to monitor the miR-144-3p level in SEV-treated NB cells. The targeted relationship between miR-144-3p and YAP1 was predicted by bioinformatics and testified by the dual-luciferase reporter assay. Results: SEV mitigated NB cell proliferation and invasion and strengthened apoptosis dose-dependently. SEV upregulated miR-144-3p. Moreover, the miR-144-3p inhibitor transfection significantly reduced the tumour-suppressive effect of SEV on NB cells. Furthermore, the dual-luciferase reporter assay confirmed that miR-144-3p targeted YAP1 and overexpressing YAP1 partially weakened the inhibitive effects of miR-144-3p on NB cells. Conclusion:SEV abated NB cell proliferation and invasion and accelerated apoptosis through the miR-144-3p/YAP1 axis.

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“…This was reflected by the elevated levels of renal MDA, NO levels along with significant decline in the antioxidant renal SOD, CAT, and GSH activities leading to functional renal impairment. Using antioxidants in our food as preventive agents against oxidative stress could block the injury induced by oxidative stress (El‐Shafey & Elsherbiny, 2020; Hoque et al, 2018). In the current study, treatment with SL and FS ameliorate CsA‐induced renal injury evident by reduction of oxidative stress and enhancement of the antioxidant status.…”

Section: Discussionmentioning

confidence: 99%

Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A

2021

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The focus of consumers in healthy food turned to the possible health benefits of particular foods and food ingredients. This study aimed to evaluate the newly fortified biscuits supplemented with sidr leaves and flaxseed and to highlight their nutritional quality and health benefits against cyclosporine A-induced dexterous effects.Sidr leaves (SL), and flaxseed (FS) were used in the preparation of fortified biscuits.Proximate analysis and sensory evaluation were carried out on the biscuits. In in vivo study, 15 male albino mice were used for each group. Groups were divided into control, CsA, SL, FS, and SL+FS-treated groups. Hematological analysis, kidney function tests, oxidative stress, and anti-oxidant status were estimated. Flow cytometry was utilized to detect apoptosis and autophagy levels. The enzyme-linked immunosorbent assay (ELISA) was used for detection of interleukin-2 (IL-2), interferonγ (IFNγ), and transforming growth factor β1 (TGF-β1) levels. The composition of biscuits complemented by SL and FS demonstrated significant improvement in the nutritional value represented by the increase in overall protein, crude fat, crude fiber, ash, and carbohydrate contents. Treatment with SL and FS restored the disturbance in hematological, kidney function, oxidative, and antioxidant biomarkers. CsA-induced apoptotic and autophagic renal cell death was suppressed. Cytokines and pro-inflammatory markers were ameliorated. The use of SL and FS in dietary products can be recommended as a functional food. Moreover, they showed renal-protective, antioxidant, anti-inflammatory, and immune-enhancing activities. Practical applicationsSidr leaves (SL) and flaxseed (FS) were used in the preparation of fortified biscuits.The composition of biscuits complemented by SL and FS demonstrated a significant improvement in the nutritional values represented by the increase in overall protein, crude fat, crude fiber, ash, and carbohydrate contents. SL and FS showed a potential therapeutic activity in reversing CsA-induced dexterous side effects by acting as an antioxidant, antiapoptotic, antiautophagic, anti-inflammatory, renal-protective, and How to cite this article: El Seedy GM, El-Shafey ES, Elsherbiny ES. Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A.

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The role of apoptosis and autophagy in the insulin-enhancing activity of oxovanadium(IV) bipyridine complex in streptozotocin-induced diabetic mice

Cited by 19 publications

References 39 publications

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

Ziziphus spina‐christi (L.) fortified with Camellia sinensis mediates apoptosis, Notch‐1 signaling, and mitigates obesity‐induced non‐alcoholic fatty liver

Sevoflurane inhibits neuroblastoma cell proliferation and invasion and induces apoptosis by miR‐144‐3p/YAP1 axis

Fortification of biscuit with sidr leaf and flaxseed mitigates immunosuppression and nephrotoxicity induced by cyclosporine A

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