Retracted: Effects of long non‐coding RNA HOST2 on cell migration and invasion by regulating MicroRNA let‐7b in breast cancer

Abstract

The study intends to investigate the effects of long non-coding RNA HOST2 (lncRNA HOST2) on cell migration and invasion by regulating microRNA let-7b (let-7b) in breast cancer. Breast cancer and adjacent normal tissues were collected from 98 patients with breast cancer. Breast cancer MCF-7 cells were divided into the blank, negative control (NC), pcDNA3-Mock, siHOST2, let-7b inhibitor, pcDNA3-HOST2, let-7b mimic, pcDNA3-HOST2 + let-7b mimic, and siHOST2 + let-7b inhibitor groups. RT-qPCR was used to detect the mRNA expressions of HOST2, let-7b, and c-Myc. Western blotting was conducted to measure the c-Myc expression. Scratch test and Transwell assay were applied to detect the cell motility, migration, and invasion. Xenograft tumor in nude mice was performed to evaluate the effect of different transfection on the tumor growth. Compared with adjacent normal tissues, HOST2 expression was higher but let-7b expression lower in breast cancer tissues. HOST2 expression in breast cancer cells was remarkably increased compared with that in the normal breast epithelial MCF-10A cells. In MCF-7 cells, in comparison with the blank and NC groups, expressions of HOST2 and c-Myc were reduced, but let-7b expression was remarkably elevated in the siHOST2 and let-7b mimic groups; the let-7b inhibitor group exhibited higher expressions of HOST2 and c-Myc but lower let-7b expression. Overexpression of HOST2 could promote cell motility, migration and invasion, thus enhancing the growth of breast cancer tumor. By inhibiting HOST2, opposite trends were found. LncRNA HOST2 promotes cell migration and invasion by inhibiting let-7b in breast cancer patients.

Long noncoding RNA HOST2, working as a competitive endogenous RNA, promotes STAT3-mediated cell proliferation and migration via decoying of let-7b in triple-negative breast cancer

“…Recently, lncRNAs had been discovered as important regulators in BC tumorgenesis [33][34][35]. HOST2 is reported playing the role of oncogene in diverse carcinomas.…”

Sinomenine hydrochloride exerts antitumor outcome in ovarian cancer cells by inhibition of long non-coding RNA HOST2 expression

“…HOST2 is a newly discovered lncRNA, and it was originally found in ovarian cancer [16] and exerted proliferative effects and migration facilitation in ovarian cancer [17]. However, accumulating evidence proposes that HOST2 also plays a part in other cancer cells, such as hepatocellular carcinoma [28], breast cancer [29,30], pancreatic cancer [31], HPV-positive cervical cancer [32] and gastric cancer [33]. Therefore, the functions of HOST2 in cancer cells deserve further research.…”

An in vitro and in vivo study of the role of long non‐coding RNA‐HOST2 in the proliferation, migration, and invasion of human glioma cells

“…The main molecular regulatory mechanism of HOST2 is to inhibit the function of tumor suppressor let‐7b, thereby increasing cancer‐like properties . HOST2 possesses a let‐7b binding site and suppresses the functions of let‐7b by serving as a molecular sponge, thereby reducing let‐7b expression . In the present study, we employed the dual luciferase gene report assay and RIP to demonstrate that HOST2 can competitively bind to let‐7b.…”