An in vitro and in vivo study of the role of long non‐coding RNA‐HOST2 in the proliferation, migration, and invasion of human glioma cells

Abstract

Gliomas are the most commonly occurring primary malignant brain tumors in the central nervous system of adults. They are rarely curable and the prognosis for high grade gliomas is generally poor. Recently, long non‐coding RNA (lncRNA) human ovarian cancer‐specific transcript 2 (HOST2) has been reported to be expressed at high levels in human ovarian cancer, involving tumorigenesis. However, little is still known about whether and how HOST2 regulates glioma development and progression. Therefore, this study aims to investigate the role of HOST2 in human glioma cells. Reverse transcription quantitative real‐time polymerase chain reaction (RT‐qPCR) was used to determine the expression of lncRNA HOST2, let‐7b, and PBX3 in human glioma cells. Cultured human glioma cells were treated with siRNA (si)‐lncRNA HOST2, let‐7b mimic, si‐lncRNA HOST2 + let‐7b inhibitor, and si‐PBX3. Parameters including cell viability, colony formation, cell migration, and cell invasion were detected by cell counting kit‐8 assay, colony formation assay, scratch test, and Transwell assay respectively to determine the effects of down‐regulated HOST2 on glioma cells. Tumor formation in nude mice was evaluated by subcutaneous tumor formation experiment. Results showed that HOST2 and PBX3 were highly expressed in glioma tissue whereas let‐7b was expressed at much lower levels. In response to treatment with si‐lncRNA HOST2, si‐PBX3, and let‐7b mimic, glioma cell lines exhibited decreased cell viability, suppressed cell migration, invasion, and reduced colony formation of glioma cells. This was accompanied by an attenuated tumor formation with smaller volume and weight in nude mice, suggesting that down‐regulated HOST2 could inhibit the tumorigenicity of glioma cells. Lastly, we found that lncRNA HOST2 was highly expressed in glioma tissues and its down‐regulation could inhibit the growth and invasion of glioma cells. © 2018 IUBMB Life, 71(1):93–104, 2019

Long noncoding RNA HOST2, working as a competitive endogenous RNA, promotes STAT3-mediated cell proliferation and migration via decoying of let-7b in triple-negative breast cancer

“…In 2015, HOST2 was firstly reported to be over-expressed in human ovarian cancer and functioned as an oncogene in ovarian cancer cells [23]. Liu D reported HOST2 was highly expressed in glioma tissues and its down-regulation could inhibit the growth and invasion of glioma cells [28]. Similar results were reported in hepatocellular carcinoma [24,25], cervical cancer [26], and gastric cancer [27].…”

“…In 2015, HOST2 was firstly reported in human ovarian cancer which is highly expressed, and function as an oncogene in epithelial ovarian cancer cells by binding to miRNA let-7b [23]. In hepatocellular carcinoma [24,25], cervical cancer [26], gastric cancer [27] and glioma [28], HOST2 was also up-regulated and played a oncogene role in tumourigenesis and development. However, there is still lack of exact mechanisms about how HOST2 experts its functions in TNBC.…”

Inhibition of microRNA let‐7b expression by KDM2B promotes cancer progression by targeting EZH2 in ovarian cancer

“…The mechanisms regulating let‐7b expression in ovarian cancer are also not well understood. Several studies have shown that long non‐coding RNA (lncRNA) HOST2 (human ovarian cancer‐specific transcript 2) sequesters let‐7b in ovarian cancer, 10 cervical cancer, 19 gliomas, 20 and breast cancer 14 . Another study demonstrated that the genomic loss of let‐7b locus is one of the reasons for the low expression of let‐7b in ovarian cancer 21 .…”

Long non‐coding RNA EPB41L4A‐AS2 suppresses progression of ovarian cancer by sequestering microRNA‐103a to upregulate transcription factor RUNX1T1

“…A total of 1000 cells were seeded into 60 mm culture dishes and then cultured with 5% CO 2 , during which the culture medium was renewed every 3 d. After 14 d, the medium was discarded, and the cells were washed 3 times with PBS, fixed with methanol for 15 min, and stained with crystal violet for 15 min. Finally, the cell colonies formed containing over 50 cells was counted under a microscope (Wang et al, 2019a).…”