Sinomenine hydrochloride exerts antitumor outcome in ovarian cancer cells by inhibition of long non-coding RNA HOST2 expression

Abstract

Background: Accumulating evidence displays that sinomenine hydrochloride (SH) are utilised to treat a variety of cancers. Nevertheless, the influences of SH on ovarian cancer stay blurry. We endeavoured to uncover the antitumor effects of SH on ovarian cancer and underlying mechanism(s). Methods: Human ovarian epithelial cell line (HOEpiC), Caov3 and SKOV3 cells were administrated with SH and/or transfection with pc-long non-coding RNA (lncRNA) human ovarian cancer-specific transcript 2 (HOST2), then cell viability, cell cycle and apoptosis and the related-proteins were respectively inspected by MTT, flow cytometry, and Western blot. In addition, expression of HOST2 was investigated by real-time PCR. Kaplan-Meier manner with the log-rank investigation was achieved to calculate overall survival. Results: SH remarkably repressed cell viability, evoked apoptosis and induced cell cycle arrest in G0/ G1. Moreover, SH statistically decreased HOST2 expression in Caov3 and SKOV3 cells. Overexpression of HOST2 significantly reversed the effects of SH on Caov3 cell viability, cell cycle and apoptosis. Clinical findings confirmed that HOST2 was profoundly higher expressed in ovarian cancer tissues and cells, and HOST2 predicated unfavourable prognosis of ovarian cancer individuals. Conclusion: Our findings recommended that SH exerted the antitumor effect in ovarian cancer cells by hindering expression of HOST2.

Sinomenine Can Inhibit the Growth and Invasion Ability of Retinoblastoma Cell through Regulating PI3K/AKT Signaling Pathway

“…As expected, sinomenine can affect the growth and apoptosis of RB cells in vitro; moreover, sinomenine also lead to decreased migration as well as invasion ability of the RB cells, and the effects of sinomenine were in a dose-dependent manner. These results were consistent with its anti-tumor behaviors in other cancers 9,[12][13][14] . Like any other type of cancer cell, RB cells 7,18) were characterized by the uncontrolled growth and increased migration and invasion ability of the tumor cells, the above observations suggested that sinomenine can inhibit the growth and metathesis of WERI-RB-1 and Y79 cells in vitro, suggesting that sinomenine can exert anti-tumor effects in RB.…”

“…In recent years, virous reports have shown the roles of traditional herbal medicine for the treatment of different type of cancers. Sinomenine is one of the main components of the traditional Chinese medicine (TCM) Sinomenium acutum 8,9) . In clinical field, sinomenine has been proved to play protective roles in cardiovascular system, immune system, as well as other tissues and organs 10,11) .…”

The Potential Diagnostic Value of Exosomal Long Noncoding RNAs in Solid Tumors: A Meta-Analysis and Systematic Review

“…However, it was later found that ncRNAs play a functional role in carcinogenesis, tumor regulation, and gene expression [17]. Furthermore, lncRNA expression always demonstrates poor prognosis in several cancers, including lung cancer [18], osteosarcoma [19], bladder cancer [20], ovarian cancer [21], liver cancer [22], gastric cancer [23], hepatocellular carcinoma [24], and breast cancer [25]. In these studies, there is an obvious connection between high-level expression of lncRNA and worse clinicopathological outcome, such as distant metastasis, tumor size, lymph node metastasis, clinical stage, and drug resistance.…”

Inferring Latent Disease-lncRNA Associations by Label-Propagation Algorithm and Random Projection on a Heterogeneous Network

“…The specific secondary structure of lncRNAs and its ability to control gene expression also render it an ideal target for drug development (Chen ZJ. et al, 2016;Tripathi et al, 2018;Xu et al, 2019). Our current understanding of the role of lncRNAs in disease is far from complete, so further understanding the relationship between lncRNAs and diseases is significant.…”