Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model

Gomes, Livia Aparecida Procópio; Figueiredo, Lívia Mara Alves; Palma, Ana Luiza do Rosário; Geraldo, Barbara Maria Corrêa; Castro, Kelly Cristine Isler; Fugisaki, Luciana Ruano de Oliveira; Jorge, Antônio Olavo Cardoso; Oliveira, Luciane Dias de; Junqueira, Juliana Campos

doi:10.1155/2016/8626987

Cited by 30 publications

(13 citation statements)

References 27 publications

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“…First, a range of the initial P. gingivalis inoculum was injected in G. mellonella at concentrations of 10 2 , 10 3 , 10 4 , 10 5 and 10 6 CFU/larva resulting in 0, 32, 93, 93 and 100% of mortality, respectively, after 168 h of infection. Similar result was found with Gomes et al [28] who evaluated the antimicrobial activity of pomegranate glycolic extract in infected G. mellonella with 10 4 cells/larvae of P. gingivalis. P. gingivalis pathogenicity is related to the production of virulence factors including biofilm formation, several varieties of lipopolysaccharide and a family of proteases known as the gingipains, many of which play a role in the development and progression of periodontal disease [29,30].…”

Section: Discussionsupporting

confidence: 89%

Immunomodulatory effect of photodynamic therapy in Galleria mellonella infected with Porphyromonas gingivalis

Santos

Alvarenga

Rossoni

et al. 2017

Microbial Pathogenesis

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Porphyromonas gingivalis is an important pathogen in the development of periodontal disease. Our study investigated if the treatment with antimicrobial photodynamic therapy (aPDT) that employs a nontoxic dye, followed by irradiation with harmless visible light can attenuate the experimental infection of P. gingivalis in Galleria mellonella. Firstly, different concentrations of P. gingivalis ranging from 10 to 10 cells/larva were injected into the animal to obtain a lethal concentration. Next, the following groups of G. mellonella infected with P. gingivalis were evaluated: inoculation of the photosensitizer and application of laser (P + L+), inoculation of physiologic solution and application of laser (P-L+), inoculation the photosensitizer without laser (P + L-) and inoculation of physiologic solution without Laser (P-L-). The effects of aPDT on infection by P. gingivalis were evaluated by survival curve analysis and hemocytes count. A lethal concentration of 10 cells/larva was adopted for evaluating the effects of aPDT on experimental infection with P. gingivalis. We found that after 120 s of PDT application, the death of G. mellonella was significantly lower compared to the control groups (p = 0.0010). Moreover, the hemocyte density in the P+L+ group was increased by 9.6 × 10 cells/mL (2.62-fold increase) compared to the infected larvae with no treatment (L-P- group) (p = 0.0175). Finally, we verified that the aPDT led to a significant reduction of the number of P. gingivalis cells in G. mellonella hemolymph. In conclusion, PDT application was effective against P. gingivalis infection by increasing the survival of G. mellonella and was able to increase the circulating hemocytes indicating that PDT activates the G. mellonella immune system.

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Section: Discussionsupporting

confidence: 89%

Immunomodulatory effect of photodynamic therapy in Galleria mellonella infected with Porphyromonas gingivalis

Santos

Alvarenga

Rossoni

et al. 2017

Microbial Pathogenesis

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“…Toxicity of SKC3 was additionally assessed in vivo in the greater wax moth larvae, G. mellonella . In recent years, G. mellonella larvae have emerged as an interesting model system for evaluating the toxicity and efficacy of novel compounds and for studying various microbial infections ( Gibreel and Upton, 2013 ; Aparecida Procopio Gomes et al, 2016 ; Skaf et al, 2017 ). The ease of handling, low maintenance costs, absence of ethical concerns, survival at human physiological temperatures are some of the advantages of using G. mellonella larvae for pre-screening of toxicity ( Tsai et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

A New Bioactive Compound From the Marine Sponge-Derived Streptomyces sp. SBT348 Inhibits Staphylococcal Growth and Biofilm Formation

et al. 2018

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Staphylococcus epidermidis, the common inhabitant of human skin and mucosal surfaces has emerged as an important pathogen in patients carrying surgical implants and medical devices. Entering the body via surgical sites and colonizing the medical devices through formation of multi-layered biofilms leads to refractory and persistent device-related infections (DRIs). Staphylococci organized in biofilms are more tolerant to antibiotics and immune responses, and thus are difficult-to-treat. The consequent morbidity and mortality, and economic losses in health care systems has strongly necessitated the need for development of new anti-bacterial and anti-biofilm-based therapeutics. In this study, we describe the biological activity of a marine sponge-derived Streptomyces sp. SBT348 extract in restraining staphylococcal growth and biofilm formation on polystyrene, glass, medically relevant titan metal, and silicone surfaces. A bioassay-guided fractionation was performed to isolate the active compound (SKC3) from the crude SBT348 extract. Our results demonstrated that SKC3 effectively inhibits the growth (MIC: 31.25 μg/ml) and biofilm formation (sub-MIC range: 1.95–<31.25 μg/ml) of S. epidermidis RP62A in vitro. Chemical characterization of SKC3 by heat and enzyme treatments, and mass spectrometry (HRMS) revealed its heat-stable and non-proteinaceous nature, and high molecular weight (1258.3 Da). Cytotoxicity profiling of SKC3 in vitro on mouse fibroblast (NIH/3T3) and macrophage (J774.1) cell lines, and in vivo on the greater wax moth larvae Galleria mellonella revealed its non-toxic nature at the effective dose. Transcriptome analysis of SKC3 treated S. epidermidis RP62A has further unmasked its negative effect on central metabolism such as carbon flux as well as, amino acid, lipid, and energy metabolism. Taken together, these findings suggest a potential of SKC3 as a putative drug to prevent staphylococcal DRIs.

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“…No estudo realizado por Aparecida Procópio Gomes et al (2016), testou-se a atividade antibacteriana da P. granatum frente a Porphyromonas gingivalis em um estudo in vivo com larvas de Galleria mellonella. Foi observado que a injeção do extrato glicólico de romã (PGE) em qualquer dose melhorou estatisticamente as taxas de sobrevivência das larvas.…”

Section: Discussionunclassified

Avaliação da atividade antibacteriana de Punica granatum Linn. (Romã) frente a bactérias orais: Uma revisão integrativa

Morais

Moura

Santos

et al. 2021

RSD

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Sabe-se que há um grande potencial de plantas medicinais com atividade antibacteriana, tornando-se alternativa para o combate às infecções. Dentre as plantas medicinais destaca-se a Punica granatum Linn. (Romã), que possui compostos bioativos utilizados para o tratamento de doenças e é utilizada como antisséptico oral para tratar a dor de garganta. Assim, o estudo apresentou como objetivo avaliar a atividade antibacteriana de Punica granatum L. frente a bactérias orais. Para isso, foi realizada uma revisão integrativa, com busca de artigos nas bases de dados PubMed, LILACS e SciELO, utilizando os termos “punica granatum”, “pomegranate”’, “dentistry”, “mouth” e “oral”. Foram selecionados artigos de acordo com os seguintes critérios para inclusão: artigos originais disponíveis na íntegra, publicados nos idiomas inglês, espanhol ou português e publicados nos últimos 10 anos (2010 a 2020). A busca resultou em 126 artigos e após leitura de título e resumo, 16 artigos atenderam aos objetivos da pesquisa e foram incluídos na revisão. Os resultados demonstram que há efeitos antibacterianos principalmente a partir de estudos in vitro (n=13; 81,25%), com ação, na maioria dos estudos da revisão, sobre Streptococcus mutans e outras espécies de Streptococcus orais. Através das análises, constata-se que diferentes formas de utilização do material da planta possuem atividade antibacteriana in vivo e in vitro, em especial o extrato da casca. Portanto, a utilização da romã tem potencial antibacteriano podendo ser uma alternativa para prevenção e terapêutica de infecções orais.

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Punica granatum L. (Pomegranate) Extract: In Vivo Study of Antimicrobial Activity against Porphyromonas gingivalis in Galleria mellonella Model

Cited by 30 publications

References 27 publications

Immunomodulatory effect of photodynamic therapy in Galleria mellonella infected with Porphyromonas gingivalis

Immunomodulatory effect of photodynamic therapy in Galleria mellonella infected with Porphyromonas gingivalis

A New Bioactive Compound From the Marine Sponge-Derived Streptomyces sp. SBT348 Inhibits Staphylococcal Growth and Biofilm Formation

Avaliação da atividade antibacteriana de Punica granatum Linn. (Romã) frente a bactérias orais: Uma revisão integrativa

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