<i>Pleurotus eryngii</i> Polysaccharide Promotes Pluripotent Reprogramming via Facilitating Epigenetic Modification

Deng, Wenwen; Cao, Xia; Wang, Yan; Yu, Qingtong; Zhang, Zhijian; Qu, Rui; Chen, Jingjing; Shao, Gang; Gao, Xiangdong; Xu, Ximing; Yu, Jiangnan

doi:10.1021/acs.jafc.5b05661

Cited by 10 publications

(7 citation statements)

References 48 publications

(69 reference statements)

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“…The colonies were released from the scaffolds and expanded on feeder layers which were then examined for pluripotent marker expression. An average of 306 cell spheres was released from each scaffold, making the reprogramming efficiency of 0.306% (from the original 1 × 10 5 cells/scaffold), which was significantly higher than that of the previously reported 2D nonviral iPSC-generating system (0.128%) . The bright-field images showcased the tightly packed morphology of the colonies on the 2D feeder layers (Figure a).…”

Section: Resultsmentioning

confidence: 75%

“…An average of 306 cell spheres was released from each scaffold, making the reprogramming efficiency of 0.306% (from the original 1 × 10 5 cells/scaffold), which was significantly higher than that of the previously reported 2D nonviral iPSCgenerating system (0.128%). 42 The bright-field images showcased the tightly packed morphology of the colonies on the 2D feeder layers (Figure 6a). In addition, the colonies from the 3D system and the human ESCs shared expression similar to that of pluripotent markers such as SSEA3, TRA-1−81, NANOG, and OCT4 (Figure 6b).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Prolonged Three-Dimensional Co-Delivery of Yamanaka Factors for Cell Reprogramming

Deng

Cao

Wang

et al. 2016

ACS Appl. Mater. Interfaces

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Reprogramming somatic cells into a pluripotent state has been widely investigated in two-dimensional (2D) systems but not described in the more biologically faithful three-dimensional (3D) scaffolds. Here, we devise a 3D porous tissue engineering scaffold that could achieve successful and efficient induction of pluripotency. To construct this 3D scaffold, nonviral hybrid nanoparticles were fabricated beforehand by employing calcium phosphate and cationized Pleurotus eryngii polysaccharide to codeliver plasmids OCT4, SOX2, KLF4 ,and C-MYC (pOSKM). These hybrid nanoparticles were then loaded into a 3D porous collagen scaffold to obtain the so-called pOSKM-activated 3D scaffold. This 3D scaffold could reprogram human umbilical cord mesenchymal stem cells (HUMSCs) into a pluripotent state, generating 3D cell spheres which showed positive expression of pluripotency markers in the 3D scaffolds and tightly packed colonies when transferred to 2D feeder layers. Besides sharing similar morphology, epigenetic modification, and expression of pluripotency genes with the embryonic stem cells, the 3D system-generated colonies could also be expanded on feeder layers for more than 20 passages, indicating the successful establishment of stable induced pluripotent stem cell (iPSC) lines. Our findings represent a first employment of porous 3D scaffolds to achieve successful reprogramming via a one-time transfection, offering a safe, simple, and effective alternative strategy for iPSC generation.

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Section: Resultsmentioning

confidence: 75%

Section: ■ Results and Discussionmentioning

confidence: 99%

Prolonged Three-Dimensional Co-Delivery of Yamanaka Factors for Cell Reprogramming

Deng

Cao

Wang

et al. 2016

ACS Appl. Mater. Interfaces

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Section: Cell Reprogramming By Nonviral Vectorsmentioning

confidence: 99%

“…Moreover, hybrid nanocarriers internalize plasmids in multiple ways, which in turn increase their reprograming efficiency. [258] Furthermore, they were applied into a 3D collagen hydrogel to produce 3D cell spheres with tightly packed cloned expressed iPSC markers. [259] Concerning reports of potential toxicity (linked to high molecular weight) of branched PEI limit their application as nanocarriers.…”

Section: Hybrid Nanocarriersmentioning

confidence: 99%

Changing Fate: Reprogramming Cells via Engineered Nanoscale Delivery Materials

Dehnavi¹,

Zadeh

Harvey

et al. 2022

Advanced Materials

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The incorporation of nanotechnology in regenerative medicine is at the nexus of fundamental innovations and early‐stage breakthroughs, enabling exciting biomedical advances. One of the most exciting recent developments is the use of nanoscale constructs to influence the fate of cells, which are the basic building blocks of healthy function. Appropriate cell types can be effectively manipulated by direct cell reprogramming; a robust technique to manipulate cellular function and fate, underpinning burgeoning advances in drug delivery systems, regenerative medicine, and disease remodeling. Individual transcription factors, or combinations thereof, can be introduced into cells using both viral and nonviral delivery systems. Existing approaches have inherent limitations. Viral‐based tools include issues of viral integration into the genome of the cells, the propensity for uncontrollable silencing, reduced copy potential and cell specificity, and neutralization via the immune response. Current nonviral cell reprogramming tools generally suffer from inferior expression efficiency. Nanomaterials are increasingly being explored to address these challenges and improve the efficacy of both viral and nonviral delivery because of their unique properties such as small size and high surface area. This review presents the state‐of‐the‐art research in cell reprogramming, focused on recent breakthroughs in the deployment of nanomaterials as cell reprogramming delivery tools.

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“…It has been reported that crude PEP could be purified by DEAE-52 cellulose and Sephadex G-100 (Chen et al, 2013;Deng et al, 2016). Herein, we successfully utilised D101 macroporous adsorptive resin and DEAE-52 cellulose to purify crude PEP to obtain two homogeneous polysaccharides.…”

Section: Monosaccharide Composition Of Ppep-1 and Ppep-2mentioning

confidence: 99%

Chemical characterisation and hypolipidaemic effects of two purified Pleurotus eryngii polysaccharides

Xing

Wang

Yang

et al. 2018

Int J of Food Sci Tech

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Summary This study is focused on the isolation and characterisation of two purified polysaccharide fractions (namely PPEP‐1 and PPEP‐2) from Pleurotus eryngii (P. eryngii) and evaluation of their hypolipidaemic effects. The Congo red analysis indicated that PPEP‐2 but not PPEP‐1 possessed a triple‐helix conformation. The atomic force microscope analysis revealed that PPEP‐1 and PPEP‐2 showed different polysaccharide chain conformations. Importantly, the mice treated with PPEP‐1 showed significantly lowered serum levels of total cholesterol (TC), triglyceride (TG) and low‐density lipoprotein cholesterol (LDL‐c) and increased high‐density lipoprotein cholesterol when compared with the hyperlipidaemia mice induced by the high‐fat diet. However, PPEP‐2 revealed less hypolipidaemic effect than PPEP‐1. Additionally, both PPEP‐1 and PPEP‐2 demonstrated remarkable hypolipidaemic effects by decreasing levels of serum TC, TG and LDL‐c in the hyperlipidaemic model induced by P‐407. Taken together, our findings suggest that the P. eryngii polysaccharides, especially PPEP‐1, could be developed as a natural functional food supplement for preventing hyperlipidaemia.

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Pleurotus eryngii Polysaccharide Promotes Pluripotent Reprogramming via Facilitating Epigenetic Modification

Cited by 10 publications

References 48 publications

Prolonged Three-Dimensional Co-Delivery of Yamanaka Factors for Cell Reprogramming

Prolonged Three-Dimensional Co-Delivery of Yamanaka Factors for Cell Reprogramming

Changing Fate: Reprogramming Cells via Engineered Nanoscale Delivery Materials

Chemical characterisation and hypolipidaemic effects of two purified Pleurotus eryngii polysaccharides

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