<i>Phyla nodiflora</i> L. Extracts Induce Apoptosis and Cell Cycle Arrest in Human Breast Cancer Cell Line, MCF-7

Teoh, Peik Lin; Liau, Monica; Cheong, Bo Eng

doi:10.1080/01635581.2018.1559942

Cited by 15 publications

(13 citation statements)

References 27 publications

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“…Breast cancer is complex and an effective cure remains elusive. Resistance to clinical drugs and radiation reduces the therapeutic effect against breast cancer and is partly attributed to the loss of apoptosis function [2]. Accordingly, apoptosis-inducing drugs may improve the therapeutic effect against breast cancer cells.…”

Section: Introductionmentioning

confidence: 99%

“…This is partly because natural products consist of many bioactive components that can suppress the function of multiple targets [3]. Natural products provide valuable resources for drug discovery for breast cancer treatment through apoptosis [2,4,5,6,7]. For example, Phyla nodiflora L. extracts were found to inhibit cell proliferation by inducing apoptosis in human breast cancer cells [2].…”

Section: Introductionmentioning

confidence: 99%

“…Natural products provide valuable resources for drug discovery for breast cancer treatment through apoptosis [2,4,5,6,7]. For example, Phyla nodiflora L. extracts were found to inhibit cell proliferation by inducing apoptosis in human breast cancer cells [2]. The study of additional natural products for drug discovery against breast cancer cells is thus warranted.…”

Section: Introductionmentioning

confidence: 99%

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Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima)

Ou‐Yang

Tsai

Tang

et al. 2019

IJMS

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Extracts from the Nepenthes plant have anti-microorganism and anti-inflammation effects. However, the anticancer effect of the Nepenthes plant is rarely reported, especially for breast cancer cells. Here, we evaluate the antitumor effects of the ethyl acetate extract of Nepenthes thorellii x (ventricosa x maxima) (EANT) against breast cancer cells. Cell viability and flow cytometric analyses were used to analyze apoptosis, oxidative stress, and DNA damage. EANT exhibits a higher antiproliferation ability to two breast cancer cell lines (MCF7 and SKBR3) as compared to normal breast cells (M10). A mechanistic study demonstrates that EANT induces apoptosis in breast cancer cells with evidence of subG1 accumulation and annexin V increment. EANT also induces glutathione (GSH) depletion, resulting in dramatic accumulations of reactive oxygen species (ROS) and mitochondrial superoxide (MitoSOX), as well as the depletion of mitochondrial membrane potential (MMP). These oxidative stresses attack DNA, respectively leading to DNA double strand breaks and oxidative DNA damage in γH2AX and 8-oxo-2′deoxyguanosine (8-oxodG) assays. Overall these findings clearly revealed that EANT induced changes were suppressed by the ROS inhibitor. In conclusion, our results have shown that the ROS-modulating natural product (EANT) has antiproliferation activity against breast cancer cells through apoptosis, oxidative stress, and DNA damage.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima)

Ou‐Yang

Tsai

Tang

et al. 2019

IJMS

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“…Both MDA-MB-231 and MDA-MB-468 cells were treated with 350 μM of RA. The chosen concentration is close to the IC 50 values in the viability study and did not show a significant necrotic effect in the apoptosis assay ( Ramachandran et al, 2005 ) ( Rahman et al, 2006 ) ( Teoh et al, 2019 ). Briefly, for each cell line, two T-75 flasks of 6 × 10 6 cells representing the control and treated cells were incubated overnight at 37 °C, followed by a 48-h treatment period.…”

Section: Methodsmentioning

confidence: 91%

Rosmarinic acid-induced apoptosis and cell cycle arrest in triple-negative breast cancer cells

Messeha

Zarmouh

Asiri

et al. 2020

European Journal of Pharmacology

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Rosmarinic acid (RA) is a polyphenolic compound with various pharmacological properties, including, anti-inflammatory, immunomodulatory, and neuroprotective, as well as having antioxidant and anticancer activities. This study evaluated the effects and mechanisms of RA in two racially different triple-negative breast cancer (TNBC) cell lines. Results obtained show that RA significantly caused cytotoxic and antiproliferative effects in both cell lines in a dose- and time-dependent manner. Remarkably, RA induced cell cycle arrest-related apoptosis and altered the expression of many apoptosis-involved genes differently. In MDA-MB-231 cells, RA arrested the cells in the G 0 /G 1 phase. In contrast, the data suggest that RA causes S-phase arrest in MDA-MB-468 cells, leading to a 2-fold increase in the apoptotic effect compared to MDA-MB-231 cells. Further, in MDA-MB-231 cells, RA significantly upregulated the mRNA expression of three genes: harakiri ( HRK ), tumor necrosis factor receptor superfamily 25 ( TNFRSF25 ), and BCL-2 interacting protein 3 ( BNIP3 ). In contrast, in the MDA-MB-468 cell line, the compound induced a significant transcription activation in three genes, including TNF , growth arrest and DNA damage-inducible 45 alpha ( GADD45A ), and BNIP3 . Furthermore, RA repressed the expression of TNF receptor superfamily 11B ( TNFRSF11B ) in MDA-MB-231 cells in comparison to the ligand TNF superfamily member 10 ( TNFSF10) and baculoviral IAP repeat-containing 5 (BIRC5) in MDA-MB-468 cells. In conclusion, the data suggest that the polyphenol RA may have a potential role in TNBC therapies, particularly in MDA-MB-468 cells.

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“…Apoptosis is morphologically characterized by chromatin condensation, DNA fragmentation, nuclear breakup [4,46], membrane blebbing [47], cell shrinkage [46,48], and formation of apoptotic bodies [49]. Apoptotic bodies are membrane-bound fragments of the dying cells that are eventually cleared by phagocytosis without any associated inflammation [50].…”

Section: Apoptosismentioning

confidence: 99%

Programmed cell death in cerebellar Purkinje neurons

Erekat¹

2022

J. Integr. Neurosci.

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Apoptosis, autophagy and necrosis are the three main types of programmed cell death. One or more of these types of programmed cell death may take place in neurons leading to their death in various neurodegenerative disorders in humans. Purkinje neurons (PNs) are among the most highly vulnerable population of neurons to cell death in response to intrinsic hereditary diseases or extrinsic toxic, hypoxic, ischemic, and traumatic injury. In this review, we will describe the three main types of programmed cell death, including the molecular mechanisms and the sequence of events in each of them, and thus illustrating the intracellular proteins that mediate and regulate each of these types. Then, we will discuss the role of Ca 2+ in PN function and increased vulnerability to cell death. Additionally, PN death will be described in animal models, namely lurcher mutant mouse and shaker mutant rat, in order to illustrate the potential therapeutic implications of programmed cell death in PNs by reviewing the previous studies that were carried out to interfere with the programmed cell death in an attempt to rescue PNs from death.

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Phyla nodiflora L. Extracts Induce Apoptosis and Cell Cycle Arrest in Human Breast Cancer Cell Line, MCF-7

Cited by 15 publications

References 27 publications

Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima)

Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima)

Rosmarinic acid-induced apoptosis and cell cycle arrest in triple-negative breast cancer cells

Programmed cell death in cerebellar Purkinje neurons

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