Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima)

Ou‐Yang, Fu; Tsai, I-Hsuan; Tang, Jen‐Yang; Yen, Ching‐Yu; Cheng, Yuan-Bin; Farooqı, Ammad Ahmad; Chen, Shu-Rong; Yu, Szu-Yin; Kao, Jun-Kai; Chang, Hsueh-Wei

doi:10.3390/ijms20133238

Cited by 19 publications

(23 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results showed that the GSH activity was decreased and SOD activity was increased with the increasing concentrations of AG-ME. These results are accordance to the previously published reports suggesting that the exposure of plant extracts could decrease the GSH [ 58 ] and increase the SOD activities [ 59 ] in MCF-7 (human breast carcinoma). The lowering of GSH activity and an increase in SOD activity indicated that oxidative stress is involved in MCF-7 cell death-induced by AG-ME.…”

Section: Discussionsupporting

confidence: 93%

Antioxidant and Anticancer Efficacies of Anethum graveolens against Human Breast Carcinoma Cells through Oxidative Stress and Caspase Dependency

Al-Oqail

Farshori

2021

BioMed Research International

View full text Add to dashboard Cite

Anethum graveolens, belonging to the family Apiaceae, has been extensively used for medicinal and therapeutic purposes since long. Plants encompass rich number of effective constituents with less toxicity. Thus, nowadays, the attempts are being made to search plant constituents that can prevent and reverse the chronic diseases, such as cancer. In this study, an in vitro antioxidant and anticancer efficacies of Anethum graveolens (AG-ME) were studied on human breast (MCF-7), lung (A-549), and cervical (HeLa) carcinoma cell lines. The antioxidant efficacies of AG-ME were evaluated by total antioxidant, DPPH radical scavenging, H2O2 scavenging, and ferrous reducing antioxidant assays. Further, the anticancer potential of AG-ME was also determined against different cancer cell lines. The AG-ME exhibited strong antioxidant activities as observed by antioxidant assays. AG-ME also showed a dose-dependent anticancer/cytotoxic potential against MCF-7, A-549, and HeLa cell lines. The AG-ME-induced reduction in GSH and increase in SOD activities indicates the role of oxidative stress in AG-ME-induced MCF-7 cell death. The results also exhibited that AG-ME triggered ROS production and significantly reduced MMP level. Moreover, a dose-dependent increase in caspase-3 and caspase-9 activities suggests that the AG-ME-induced MCF-7 cell death is caspase-dependent. Together, the present study provides reasoning and reassurance for the uses of A. graveleons for medical purposes as an antioxidant and anticancer agent. Additional investigations are required to examine biological and anticancer activities under an in vivo system to discover a possible beneficial use of AG-ME against diseases.

show abstract

Section: Discussionsupporting

confidence: 93%

Antioxidant and Anticancer Efficacies of Anethum graveolens against Human Breast Carcinoma Cells through Oxidative Stress and Caspase Dependency

Al-Oqail

Farshori

2021

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…To provide a comparison with the clinically used anticancer drug cisplatin, the IC 50 value for cisplatin at 24 h in the MTS assay for SKBR3 cells was 49.8 μM [ 34 ]. The IC 50 concentrations for 48 h cisplatin in the ATP assay were 4.9, 17.9, and 26.9 μM for SKBR3, MCF7, and MDA-MB-231 cells, respectively [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Physalis peruviana-Derived Physapruin A (PHA) Inhibits Breast Cancer Cell Proliferation and Induces Oxidative-Stress-Mediated Apoptosis and DNA Damage

Cheng

Lin

et al. 2021

Antioxidants

Self Cite

View full text Add to dashboard Cite

Breast cancer expresses clinically heterogeneous characteristics and requires multipurpose drug development for curing the different tumor subtypes. Many withanolides have been isolated from Physalis species showing anticancer effects, but the anticancer function of physapruin A (PHA) has rarely been investigated. In this study, the anticancer properties of PHA in breast cancer cells were examined by concentration and time-course experiments. In terms of cellular ATP content, PHA inhibited the proliferation of three kinds of breast cancer cells: MCF7 (estrogen receptor (ER)+, progesterone receptor (PR)+/−, human epidermal growth factor receptor 2 (HER2)−), SKBR3 (ER−/PR−/HER2+), and MDA-MB-231 (triple-negative). Moreover, PHA induced G2/M arrest in MCF7 and MDA-MB-231 cells. In terms of flow cytometry, PHA induced the generation of reactive oxygen species (ROS), the generation of mitochondrial superoxide, mitochondrial membrane potential depletion, and γH2AX-detected DNA damage in breast cancer MCF7 and MDA-MB-231 cells, which were suppressed by the ROS inhibitor N-acetylcysteine (NAC). In terms of flow cytometry and Western blotting, PHA induced apoptotic expression (annexin V, and intrinsic and extrinsic apoptotic signaling), which was suppressed by NAC and an apoptosis inhibitor (Z-VAD-FMK), in breast cancer cells. Therefore, PHA is a potential anti-breast-cancer natural product that modulates the oxidative-stress response, cell-cycle disturbance, apoptosis, and γH2AX-detected DNA damage.

show abstract

“…For comparison with other clinical drugs, the IC 50 value of cisplatin in oral cancer OECM-1 and Ca9-22 cells are 2.61 and 1.20 μg/mL in a 48 h MTS assay ( Figure 2 B). At 48 h MTS assay, the IC 50 value of cisplatin in breast cancer SKBR3 and MCF7 cells are 3.71 and 10.45 μg/mL, respectively [ 33 ]. Accordingly, the crude extract MEUB shows a similar sensitivity to cisplatin in oral cancer cells and a higher sensitivity than in breast cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress

Tang

et al. 2020

Antioxidants

Self Cite

View full text Add to dashboard Cite

Some lichens provide the resources of common traditional medicines and show anticancer effects. However, the anticancer effect of Usnproliea barbata (U. barbata) is rarely investigated, especially for oral cancer cells. The aim of this study was to investigate the cell killing function of methanol extracts of U. barbata (MEUB) against oral cancer cells. MEUB shows preferential killing against a number of oral cancer cell lines (Ca9-22, OECM-1, CAL 27, HSC3, and SCC9) but rarely affects normal oral cell lines (HGF-1). Ca9-22 and OECM-1 cells display the highest sensitivity to MEUB and were chosen for concentration effect and time course experiments to address its cytotoxic mechanisms. MEUB induces apoptosis of oral cancer cells in terms of the findings from flow cytometric assays and Western blotting, such as subG1 accumulation, annexin V detection, and pancaspase activation as well as poly (ADP-ribose) polymerase (PARP) cleavage. MEUB induces oxidative stress and DNA damage of oral cancer cells following flow cytometric assays, such as reactive oxygen species (ROS)/mitochondrial superoxide (MitoSOX) production, mitochondrial membrane potential (MMP) depletion as well as overexpression of γH2AX and 8-oxo-2′deoxyguanosine (8-oxodG). All MEUB-induced changes in oral cancer cells were triggered by oxidative stress which was validated by pretreatment with antioxidant N-acetylcysteine (NAC). In conclusion, MEUB causes preferential killing of oral cancer cells and is associated with oxidative stress, apoptosis, and DNA damage.

show abstract

Antiproliferation for Breast Cancer Cells by Ethyl Acetate Extract of Nepenthes thorellii x (ventricosa x maxima)

Cited by 19 publications

References 41 publications

Antioxidant and Anticancer Efficacies of Anethum graveolens against Human Breast Carcinoma Cells through Oxidative Stress and Caspase Dependency

Antioxidant and Anticancer Efficacies of Anethum graveolens against Human Breast Carcinoma Cells through Oxidative Stress and Caspase Dependency

Physalis peruviana-Derived Physapruin A (PHA) Inhibits Breast Cancer Cell Proliferation and Induces Oxidative-Stress-Mediated Apoptosis and DNA Damage

Methanol Extract of Usnea barbata Induces Cell Killing, Apoptosis, and DNA Damage against Oral Cancer Cells through Oxidative Stress

Contact Info

Product

Resources

About