<i>Orostachys japonicus</i> induce p53‐dependent cell cycle arrest through the MAPK signaling pathway in OVCAR‐3 human ovarian cancer cells

Lee, Kyung Sun; Kim, Suhng Wook; Lee, Hyeong Seon

doi:10.1002/fsn3.836

Cited by 17 publications

(11 citation statements)

References 33 publications

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“…An abnormal cell cycle may lead to the occurrence of a variety of different types of tumor, such as hepatocellular carcinoma (33,34), breast cancer (35), pancreatic cancer (36), ovarian cancer (37) and NSCLC in non-smoking females (38). Wang et al (39) used different microarray expression profiles in their study of primitive neuro-ectodermal tumors, and revealed the same four pathways that were identified in the present study, and several of the same genes in the modules that they identified as being important.…”

Section: Discussionmentioning

confidence: 99%

Identification of candidate genes associated with the pathogenesis of small cell lung cancer via integrated bioinformatics analysis

et al. 2019

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The pathogenesis of small cell lung cancer (SCLC), a highly metastatic malignant tumor, remains unclear. In the present study, important genes and pathways that are involved in the pathogenesis of SCLC were identified. The following four datasets were downloaded from the Gene Expression Omnibus: GSE60052, GSE43346, GSE15240 and GSE6044. The differentially expressed genes (DEGs) between the SCLC samples and the normal samples were analyzed using R software. The limma package was used for every dataset. The RobustRankAggreg package was used to integrate the DEGs from the four datasets. Functional and pathway enrichment analyses were conducted using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases with FunRich software and R software, respectively. In addition, the protein-protein interaction (PPI) network of the DEGs was constructed using the STRING database and Cytoscape software. Hub genes and significant modules were identified using Molecular Complex Detection in Cytoscape software. Finally, the expression values of hub genes were determined using the Oncomine online database. In total, 412 DEGs were identified following the integration of the four datasets, with 146 upregulated genes and 266 downregulated genes. The upregulated DEGs were primarily enriched in the cell cycle, cell division and microtubule binding. The downregulated DEGs were primarily enriched in the complement and coagulation cascades, the cytokine-mediated signaling pathway and protein binding. Eight hub genes and 1 significant module correlated to the cell cycle pathway were identified based on a subset of the PPI network. Finally, five hub genes were identified as highly expressed in SCLC tissue compared with normal tissue. The cell cycle pathway may be the pathway most closely associated with the pathogenesis of SCLC. NDC80, BUB1B, PLK1, CDC20 and MAD2L1 should be the focus of follow-up studies regarding the diagnosis and treatment of SCLC.

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Section: Discussionmentioning

confidence: 99%

Identification of candidate genes associated with the pathogenesis of small cell lung cancer via integrated bioinformatics analysis

et al. 2019

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“…The O. japonicus was separated sequentially with organic solvents. These soluble fractions were examined for the effect of anticancer in various cells such as human gastric, hepatoma, colon, ovarian, and pancreatic cancer (Kim, Nam, Kim, Ryu, & Lee, ; Lee, Lee, Kim, Kim, et al, ; Lee et al, ; Lee, Lee, Kim, Suk, et al, ; Ryu, Lee, Kwon, & Lee, ; Ryu et al, ). Among these fractions, EtOAc and DCM extracts exhibited the highest effect for apoptosis signaling pathways.…”

Section: Resultsmentioning

confidence: 99%

“…Fractionated O. japonicus was supplied from a farm in Miryang (Geobugiwasong Ltd.). The O. japonicus was separated using organic solvents, and the extract method was described in the previous studies (Lee, Lee, Kim, Kim, et al, ; Lee, Kim, & Lee, ; Lee, Lee, Kim, Suk, et al, ; Ryu et al, ). Each fraction removed the solvents by evaporator at 40°C to dryness.…”

Section: Methodsmentioning

confidence: 99%

Orostachys japonicus extract inhibits the lipopolysaccharide‐induced pro‐inflammatory factors by suppression of transcription factors

Lee

2020

Food Science & Nutrition

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Orostachys japonicus (O. japonicus) was extracted with ethanol (EtOH) and sequentially separated with organic solvents, including n‐hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc), n‐butanol (BuOH), and water (H2O). All the fractions were confirmed for anti‐inflammatory activity in an inflammatory condition. The DCM fraction showed the highest anti‐inflammatory ability. Here, we examined the effect of DCM fraction and investigated the intracellular signaling pathways in LPS‐stimulated RAW 264.7 macrophage cells. The DCM fraction significantly inhibited the mRNA levels of pro‐inflammatory mediators and cytokines including iNOS, COX‐2, IL‐1β, IL‐2, IL‐6, and IP‐10 in LPS‐stimulated cells. Also, the treatment of DCM fraction excellently reduced the expression of the proteins of AP‐1 (phospho‐c‐Jun and phospho‐c‐Fos) and phospho‐IRF3 as transcription factors. As a result, it suppressed LPS‐induced inflammatory mediator and cytokines via inhibition of transcription factors. In conclusion, our data demonstrated that DCM fraction has a strong anti‐inflammatory activity that improves the inflammatory state.

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“…Moreover, ERK1/2, along with PI3K and p38 signaling pathways, has been shown to promote chondrosarcoma cell motility, invasion and lung colonization (58). The ERK1/2 pathway affects cell cycle progression and apoptosis through the modulation of specific cell cycle regulators expression, including p53 and p21 in OVCAR-3 human ovarian and MCF-7 breast cancer cells (59,60). In the present study, p53 expression was elevated in the HTB94 cells, in a manner dependent on active ERK1/2 signaling.…”

Section: Discussionmentioning

confidence: 99%

Lumican mediates HTB94 chondrosarcoma cell growth via an IGF‑IR/Erk1/2 axis

et al. 2020

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Chondrosarcoma is a malignant bone tumor characterized by the production of a modified cartilage-type extracellular matrix (ECM). In the present study, the expression levels of the small leucine-rich proteoglycans (SLRPs), decorin, biglycan and lumican, were examined in the HTB94 human chondrosarcoma cell line. HTB94 cells were found to express and secrete the 3 SLRP members. RT-qPCR and western blot analysis demonstrated that lumican was the most abundantly secreted SLRP, whereas decorin and biglycan expression levels were low. The utilization of short interfering RNA specific for the decorin, biglycan, and lumican genes resulted in the efficient downregulation of the respective mRNA levels (P≤0.001). The growth of the HTB94 cells was stimulated by lumican (P≤0.001), whereas their migration and adhesion were not affected (P=NS). By contrast, these cellular functions were not sensitive to a decrease in low endogenous levels of decorin and biglycan. Lumicandeficiency significantly inhibited both basal and insulin-like growth factor I (IGF-I)-induced HTB94 cell growth (P≤0.001 andP≤0.01, respectively). These effects were executed through the insulin-like growth factor I receptor (IGF-IR), whose activation was markedly attenuated (P≤0.01) in lumican-deficient HTB94 cells. The downregulation of lumican induced the substantial inhibition of extracellular regulated kinase (ERK1/2) activation (P≤ 0.01), indicating that ERK1/2 is a necessary component of lumican/IGF-IR-mediated HTB94 cell proliferation. Moreover, the lumican-deficient cells exhibit increased mRNA levels of p53 (P≤0.05), suggesting that lumican facilitates HTB94 cell growth through an IGF-IR/ERK1/2/p53 signaling cascade. On the whole, the findings of the present study demonstrate that endogenous lumican is a novel regulator of HTB94 cell growth.

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Orostachys japonicus induce p53‐dependent cell cycle arrest through the MAPK signaling pathway in OVCAR‐3 human ovarian cancer cells

Cited by 17 publications

References 33 publications

Identification of candidate genes associated with the pathogenesis of small cell lung cancer via integrated bioinformatics analysis

Identification of candidate genes associated with the pathogenesis of small cell lung cancer via integrated bioinformatics analysis

Orostachys japonicus extract inhibits the lipopolysaccharide‐induced pro‐inflammatory factors by suppression of transcription factors

Lumican mediates HTB94 chondrosarcoma cell growth via an IGF‑IR/Erk1/2 axis

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