Nitraria retusafruit prevents penconazole-induced kidney injury in adult rats through modulation of oxidative stress and histopathological changes

Abstract

Context:Nitraria retusa (Forssk.) Asch. (Nitrariaceae) is a medicinal plant which produces edible fruits whose antioxidant activity has been demonstrated.Objective: The current study elucidates the potential protective effect of N. retusa fruit aqueous extract against nephrotoxicity induced by penconazole, a triazole fungicide, in the kidney of adult rats.Materials and methods: Adult Wistar rats were exposed either to penconazole (67 mg/kg body weight), or to N. retusa extract (300 mg/kg body weight) or to their combination. Penconazole was administered by intra-peritoneal injection every 2 days from day 7 until day 15, the sacrifice day, while N. retusa extract was administered daily by gavage during 15 days. Oxidative stress parameters, kidney biomarkers and histopathological examination were determined.Results:Nitraria retusa extract administration to penconazole treated rats decreased kidney levels of malondialdehyde (−10%), hydrogen peroxide (−12%), protein carbonyls (PCOs, −11%) and advanced oxidation protein products (AOPP, −16%); antioxidant enzyme activities: catalase (−13%), superoxide dismutase (−8%) and glutathione peroxidase (GPx, −14%), and the levels of non-enzymatic antioxidants: non-protein thiols (−9%), glutathione (−7%) and metallothionein (−12%). Furthermore, this plant extract prevented kidney biomarker changes by reducing plasma levels of creatinine, urea, uric acid and LDH and increasing those of ALP and GGT. Histopathological alterations induced by penconazole (glomeruli fragmentation, Bowman’s space enlargement, tubular epithelial cells necrosis and infiltration of inflammatory leucocytes) were attenuated following N. retusa administration.Discussion and conclusion: Our results indicated that N. retusa fruit extract had protective effects against penconazole-induced kidney injury, which could be attributed to its phenolic compounds.

Antioxidant capacity of Nitraria retusa leaf extracts against mitomycin C-induced genetic toxicity in male mice

“…For our best of knowledge, there are no published data regarding the effect of Nitraria retusa extract on the anti-genotoxicity in in vivo mammalian cells. However, Chaâbane et al (2017) reported that N. retusa fruit extract exhibited a highly protective action against toxic effect of penconazole which induce kidney injury. They suggested that the protective action of N. retusa fruit extract could result from its content of the phenolic compounds.…”

Efficacy of Allium sativum oil to alleviate tebuconazol-induced oxidative stress in the liver of adult rats

“…The exposure to TEB caused a significant decrease in the body weight gain, which could be explained either by the decrease in food consumption and/or by the toxicity induced by this toxicant (42). However, we observed a significant increase in water consumption as shown in TEB-treated groups, which could be justified by the mechanism to counteract the toxicity induced by this fungicide (11). The additional effects of TEB treatment revealed an increase in the relative liver weight.…”

“…They act as free radical scavengers preventing cells and tissue damage. Exogenous antioxidants obtained from natural sources are considered relatively safe and without undesirable side effects (11) Many medicinal plants have interesting biological and pharmacological activities and are used as therapeutic agents (12). Garlic (Allium sativum L.) belongs to the Alliaceae family (13).…”

Effects of Hydroalcoholic Extract of <em>Celtis iguanaea </em>on Markers of Cardiovascular Diseases and Glucose Metabolism in Cholesterol-Fed Rats

“…SOD enzyme catalyzes the dismutation of superoxide anions into hydrogen peroxide (H2O2) that easily diffuses across cell membranes and cytosol. To attenuate this action, CAT and other enzymes are able to neutralize this reactive species, producing water (H2O) and molecular oxygen [55,56], while NPSH groups protect the cells against the cytotoxic effects of endogenous or exogenous electrophiles metabolites [57]. Thus, the increase of these antioxidants may have contributed to protect against lipid peroxidation in plasma.…”