Antioxidant capacity of Nitraria retusa leaf extracts against mitomycin C-induced genetic toxicity in male mice

Abstract

Background: Recently, a great interest in the usage of new derived drugs extracted from plant has been increased to treat and/or prevent several diseases including cancer. Therefore, the present study was conducted to use Nitraria retusa leaf extract to increase the antioxidant activity and reduce the genotoxic activity of chemotherapeutic drugs mitomycin C (MMC) in male mice. Male Swiss albino mice (n = 80) were divided in several experimental groups and treated with single dose of MMC with supplementation of 50 and 100 mg/kg body wt Nitraria retusa leaf extract for several time intervals (3 days, 1 week, and 1 month). Results: This study found that MMC-treated mice observed significant increases in the frequency of chromosomal abnormalities in both cell types including bone marrow and spermatocyte cells, induce the mean values of DNA damage, and decrease the expression values of antioxidant enzyme-related genes (CAT, GPx, and GSTT1) compared with control mice. However, supplementation of MMC-treated mice with Nitraria retusa leaf extract suppressed the harmful impacts induced by MMC especially with the high dose and longer duration of the administration. Conclusion: The results suggested that the protective capacity of the Nitraria retusa leaf extract in protecting the hepatic cells could be attributed to the presence of isorhamnetin 3-o-robinobioside, palmitic acid, and β-sitosterols in its extract which are known for their anti-tumor and anti-mutagenic activities.

Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga Fucus vesiculosus in Mice

“…In the present study, MMC induced a high and statistically significant percentage of chromosome aberrations in mouse bone marrow cells. These finding are in agreement with those observed by Khalil et al [67] who documented that MMC induced chromosomal aberrations in Swiss albino mice. MMC has been shown to cause mitotic delay [68] which could affect the yield of chemically induced abnormalities [69].…”

The Bioremediation Capacity of Sphingomonas melonis for Methomyl‐Contaminated Soil Media: RSM Optimization and Biochemical Assessment by Dreissena polymorpha

“…AChE activity was inhibited with increasing concentration compared to control, [1,32] investigated the effect of the pesticide methomyl on different enzymatic activities in Cyprinus carpio L. Brain AChE activity was significantly reduced after 96 hours of exposure compared to controls throughout the experimental periods. Khalil et al [33] investigated the acute and non-lethal effects of methomyl lanate on the land snail Eobania vermiculata. They suggested that AChE activity of E. vermiculata was dose-dependently inhibited by carbamate lannate.…”