Myroides profundisp. nov., isolated from deep-sea sediment of the southern Okinawa Trough

Abstract

A Gram-negative, nonmotile, aerobic and oxidase- and catalase-positive bacterium, designated D25T, was isolated from the deep-sea sediments of the southern Okinawa Trough area. Phylogenetic analyses of 16S rRNA gene sequences showed that strain D25T fell within the genus Myroides, with 99.2%, 96.0% and 93.4% sequence similarities to the only three recognized species of Myroides. However, the DNA-DNA similarity value between strain D25T and its nearest neighbour Myroides odoratimimus JCM 7460T was only 49.9% (<70%). Several phenotypic properties could be used to distinguish strain D25T from other Myroides species. The main cellular fatty acids of strain D25T were iso-C15:0, iso-C17:1omega9c, iso-C17:03-OH and Summed Feature 3 (comprising C16:1omega7c and/or iso-C15:02-OH). The major respiratory quinone was MK-6. The DNA G+C content was 33.0 mol%. The results of the polyphasic taxonomy analysis suggested that strain D25T represents a novel species of the genus Myroides, for which the name Myroides profundi sp. nov. is proposed. The type strain is D25T (=CCTCC M 208030T=DSM 19823T).

Characterization of a Novel Subtilisin-like Protease Myroicolsin from Deep Sea Bacterium Myroides profundi D25 and Molecular Insight into Its Collagenolytic Mechanism

“…Bacterial Strains, Plasmids, and Experimental Materials-M. profundi D25 isolated from the deep sea sediment of the southern Okinawa Trough was used for protease production (32). Escherichia coli DH5␣ (Transgen Biotech) was used as the host for gene cloning.…”

Antibiotic resistance mechanisms of Myroides sp.

“…nov. (Cho et al, 2011), strain SM1(T), named M. pelagicus sp. nov. (Yoon et al, 2006)), deep-sea sediment (strain D25T (Zhang et al, 2008)), human saliva (strain MY15T, named M. phaeus sp. nov. (Yan et al, 2012)), as well as strains from urine, sputum, surgical exudate (Andreoni, 1986), and patients' matter (Table 1).…”

“…at the species level in most cases. However, they and 16S ribosomal RNA gene sequencing (16S rRNA sequencing), a standardized bacterial strain identification method (Yoon et al, 2006;Zhang X.Y. et al, 2008;Zhang Z.D.…”

Sepsis caused by Myroides spp. associated with bronchopneumonia and coinfection with Pseudomonas aeruginosa and Proteus vulgaris: A case report and review of Myroides spp. infections

“…Phenotypically, there are two different species, M. odoratus (which does not grow on MacConkey agar culture medium) and M. odoratimimus. In recent years, other 3 species were isolated from seawater [3][4][5], respectively M. marinus, M. profundi and M. pelagicus. It is considered that infection in humans is due to the takeover of bacteria from the environment, contaminated soil or water; in humans, Myroides spp.…”