Bacteria of the genus Myroides (Myroides spp.) are rare opportunistic pathogens. Myroides sp. infections have been reported mainly in China. Myroides sp. is highly resistant to most available antibiotics, but the resistance mechanisms are not fully elucidated. Current strain identification methods based on biochemical traits are unable to identify strains accurately at the species level. While 16S ribosomal RNA (rRNA) gene sequencing can accurately achieve this, it fails to give information on the status and mechanisms of antibiotic resistance, because the 16S rRNA sequence contains no information on resistance genes, resistance islands or enzymes. We hypothesized that obtaining the whole genome sequence of Myroides sp., using next generation sequencing methods, would help to clarify the mechanisms of pathogenesis and antibiotic resistance, and guide antibiotic selection to treat Myroides sp. infections. As Myroides sp. can survive in hospitals and the environment, there is a risk of nosocomial infections and pandemics. For better management of Myroides sp. infections, it is imperative to apply next generation sequencing technologies to clarify the antibiotic resistance mechanisms in these bacteria.
Shale
gas is a promising energy source offering additional energy
security over concerns of fossil fuel depletion. Injecting CO
2
into depleted shale gas reservoirs might provide a feasible
solution for CO
2
storage and enhanced gas recovery. However,
shale strain caused by the CO
2
injection as well as CO
2
sequestration in the reservoir needs to be considered during
shale gas production. For this purpose, this paper examines the adsorption
capacities, CO
2
-induced swelling, and He-induced strain
of shales at 0–16 MPa and 35–75 °C. The maximum
excess adsorption at different temperatures correlated with the bulk
phase density: as the CO
2
temperature increased, the maximum
excess adsorption density decreased. The density of the adsorbed phase,
obtained using the Dubinin–Radushkevich model, was used to
fit the excess adsorption data. At low pressure, the CO
2
-induced strain on shale was caused by the gas adsorption, whereas
at high pressure, it was caused by gas pressure. The absolute adsorption
linearly correlated with the adsorption-induced strain.
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