Antibiotic resistance mechanisms of Myroides sp.

Hu, Shaohua; Yuan, Shu-xing; Qu, Hai; Jiang, Tao; Zhou, Yajun; Wang, Mingxi; Ming, Desong

doi:10.1631/jzus.b1500068

Cited by 49 publications

(53 citation statements)

References 37 publications

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“…We reported a case of urosepsis due to M. odoratimimus in a 41 year old male. Resistance to multiple antibiotics in Myroides spp, especially intrinsic resistance to betalactam antibiotics has been reported previously (Mammeri et al, 2002, Hu et al, 2016. The present isolate was resistant to all available antibiotics except minocycline.…”

Section: Resultssupporting

confidence: 68%

Urosepsis due to Multi Drug Resistant Myroides odoratimimus: A Case Report

Ranjan¹,

Karade²,

Rahi³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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A 41 year old male; a known case of frontal astrocytoma was brought to this centre with history of headache, altered sensorium and an episode of seizure. He was diagnosed as a case of anaplastic astrocytoma six months back and underwent surgical resection followed by chemotherapy and radiotherapy. Clinical evaluation revealed bedridden but responsive patient with Glasgow coma scale of 6/15. Radiological investigations confirmed recurrence of lesion in right frontal lobe. He was admitted to the intensive care unit (ICU) and underwent emergent surgical resection of the tumor.The patient had a stormy post-operative course, requiring ventilatory as well as ionotropic support. Histopathological examination of the resected tumor specimen confirmed a grade III anaplastic astrocytoma. On third post-operative day, he developed continuous, moderate to high grade fever with features of hemodynamic instability. Total leucocytes count increased from 11500/cumm on first post-operative day to 16,200/cumm. Peripheral blood smear examination indicated neutrophilia with left shift and toxic granulation. Urine examination showed presence of traces of albumin, reducing substances and numerous pus cells. Blood, urine and surgical drain samples were sent to microbiology laboratory for culture and antibiotic sensitivity testing. International Journal of Current Microbiology and Applied Sciences

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Section: Resultssupporting

confidence: 68%

Urosepsis due to Multi Drug Resistant Myroides odoratimimus: A Case Report

Ranjan¹,

Karade²,

Rahi³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…All bacterial hosts of the chromosomal genes in this group, both the representative genes and the members of the families, belonged to the phylum Bacteroidetes. Two of the families in this group consisted of genes located in pathogens, where G29 in family 22 was found on the chromosome of an isolate from the poultry pathogen Ornithobacterium rhinotracheale [37] and G04 in family 4 was present on a plasmid harbored by an isolate of the opportunistic pathogen Myroides odoratimimus [38]. …”

Section: Resultsmentioning

confidence: 99%

Identification of 76 novel B1 metallo-β-lactamases through large-scale screening of genomic and metagenomic data

et al. 2017

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BackgroundMetallo-β-lactamases are bacterial enzymes that provide resistance to carbapenems, the most potent class of antibiotics. These enzymes are commonly encoded on mobile genetic elements, which, together with their broad substrate spectrum and lack of clinically useful inhibitors, make them a particularly problematic class of antibiotic resistance determinants. We hypothesized that there is a large and unexplored reservoir of unknown metallo-β-lactamases, some of which may spread to pathogens, thereby threatening public health. The aim of this study was to identify novel metallo-β-lactamases of class B1, the most clinically important subclass of these enzymes.ResultsBased on a new computational method using an optimized hidden Markov model, we analyzed over 10,000 bacterial genomes and plasmids together with more than 5 terabases of metagenomic data to identify novel metallo-β-lactamase genes. In total, 76 novel genes were predicted, forming 59 previously undescribed metallo-β-lactamase gene families. The ability to hydrolyze imipenem in an Escherichia coli host was experimentally confirmed for 18 of the 21 tested genes. Two of the novel B1 metallo-β-lactamase genes contained atypical zinc-binding motifs in their active sites, which were previously undescribed for metallo-β-lactamases. Phylogenetic analysis showed that B1 metallo-β-lactamases could be divided into five major groups based on their evolutionary origin. Our results also show that, except for one, all of the previously characterized mobile B1 β-lactamases are likely to have originated from chromosomal genes present in Shewanella spp. and other Proteobacterial species.ConclusionsThis study more than doubles the number of known B1 metallo-β-lactamases. The findings have further elucidated the diversity and evolutionary history of this important class of antibiotic resistance genes and prepare us for some of the challenges that may be faced in clinics in the future.Electronic supplementary materialThe online version of this article (10.1186/s40168-017-0353-8) contains supplementary material, which is available to authorized users.

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“…The numbers of genes, proteins, and tRNAs in PR63039 genome were larger than that of the other nine genomes. We should mention that eight Myroides genomes were incomplete, and the sequencing of the plasmids in PR63039 strains were not completed even its chromosome was fully sequenced (Figure 1 in Hu et al., , ).…”

Section: Resultsmentioning

confidence: 99%

“…M. odoratimimus infections are life‐threatening due to its multidrug resistance and unknown pathogenicity (as summarized in Hu et al., ). In our previous report (Hu et al., ), to some extent, we correlated the phenotype in antibiotic susceptibilities and infectivity of M. odoratimimus with the genomic findings of a variety of resistance genes, virulence factor (VF) genes.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic analysis of Myroides odoratimimus isolates

Chen

Yi-yin

et al. 2018

MicrobiologyOpen

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Myroides odoratimimus is an important nosocomial pathogen. Management of M. odoratimimus infection is difficult owing to the multidrug resistance and the unknown pathogenesis mechanisms. Based on our previous genomic sequencing data of M. odoratimimus PR63039 (isolated from a patient with the urinary tract infection), in this study, we further performed comparative genomic analysis for 10 selected Myroides strains. Our results showed that these Myroides genome contexts were very similar and phylogenetically related. Various prophages were identified in the four clinical isolate genomes, which possibly contributed to the genome evolution among the Myroides strains. CRISPR elements were only detected in the two clinical (PR63039 and CCUG10230) isolates and two environmental (CCUG12700 and H1bi) strains. With more stringent cutoff parameters in CARD analysis, the four clinical M. odoratimimus contained roughly equal antibiotic resistance genes, indicating their similar antibiotic resistance profiles. The three clinical (CCUG10230, CCUG12901, CIP101113) and three environmental (CCUG12700, L41, H1bi) M. odoratimimus strains were speculated to carry the indistinguishable virulent factors (VFs), which may involve in the similar pathogenesis mechanism. Moreover, some VFs might confer to the high capacity of dissemination, attacking tissue cells and induction of autoimmune complications. Our results facilitate the research of antibiotic resistance and the development of therapeutic regimens for the M. odoratimimus infections.

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Antibiotic resistance mechanisms of Myroides sp.

Cited by 49 publications

References 37 publications

Urosepsis due to Multi Drug Resistant Myroides odoratimimus: A Case Report

Urosepsis due to Multi Drug Resistant Myroides odoratimimus: A Case Report

Identification of 76 novel B1 metallo-β-lactamases through large-scale screening of genomic and metagenomic data

Comparative genomic analysis of Myroides odoratimimus isolates

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