<i>miR-497</i> regulates fatty acid synthesis <i>via</i> LATS2 in bovine mammary epithelial cells

Chen, Zhi; Chu, Shuangfeng; Liang, Yusheng; Xu, Tianle; Sun, Yujia; Li, Mingxun; Zhang, Huimin; Wang, Xiaolong; Mao, Yongjiang; Loor, Juan J.; Yun, Wu; Yang, Zhangping

doi:10.1039/d0fo00952k

Cited by 22 publications

(17 citation statements)

References 34 publications

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“…Ten microliters were used for bicinchoninic acid (BCA) protein detection. Finally, the BCA protein value was used for verification …”

Section: Methodsmentioning

confidence: 99%

“…Finally, the BCA protein value was used for verification. 29 Fatty Acid Content Detection. The extraction and methyl esterification of intracellular fatty acids were performed according to previous studies.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The extraction and methyl esterification of intracellular fatty acids were performed according to previous studies. 29 Briefly, 2 mL of a 0.25% sulfuric acid methanol solution was added to 100 mg of cells, and after ultrasonication, the cells were incubated at 80 °C for 1 h to methylate the fatty acids. The supernatant of the sample was transferred to a siliconized glass centrifuge tube and vortexed to remove water.…”

Section: ■ Introductionmentioning

confidence: 99%

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Circ11103 Interacts with miR-128/PPARGC1A to Regulate Milk Fat Metabolism in Dairy Cows

Chen

Liang

et al. 2021

J. Agric. Food Chem.

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The quality of milk is inseparable from its milk components, and fatty acid content is a key factor affecting the quality of milk. In this study, the miRNA and mRNA profiles of the bovine mammary gland tissue during the dry period and the peak lactation period were determined through high-throughput sequencing. In total, 72 miRNA−mRNA regulatory pathways were screened, including miR-128/PPARGC1A regulatory pathways. miR-128 can directly target PPARGC1A and inhibit its expression. In addition, the study also observed that there was a miR-128 binding site in the sequence of the circular RNA circ11103, and circ11103 significantly reduced the expression of miR-128. circ11103 upregulated the triglyceride levels in bovine mammary epithelial cells (BMECs) and increased the contents of unsaturated fatty acids. However, miR-128 decreased triglyceride and cholesterol levels in BMECs. This study aims to analyze the mechanism governing the regulatory effect of circ11103 on milk fat metabolism, which provides new insights into improving milk quality.

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“…Ten microliters were used for bicinchoninic acid (BCA) protein detection. Finally, the BCA protein value was used for verification …”

Section: Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Circ11103 Interacts with miR-128/PPARGC1A to Regulate Milk Fat Metabolism in Dairy Cows

Chen

Liang

et al. 2021

J. Agric. Food Chem.

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“…Methylation of the promoter region of miR-338-5p-5p can also lead to low levels of miR-338-5p-5p in astrocytoma. The miRNA is an important type of small molecule non-coding RNA, which can not only be used as an anti-tumor treatment strategy (for example, in this study, the entire sequence of hsa-pre-miR-338-5p was prepared as rotavirus/lentivirus), but also the deficiency of miRs would be the possible mechanisms for the aberrant expression of its target gene (some pro-oncogene) in malignant tumor tissues (26,(52)(53)(54). In terms of molecular mechanism, miRNA is used in the 3'UTR region of its target gene through a sequence-specific mechanism (55)(56)(57)(58)(59).…”

Section: Discussionmentioning

confidence: 99%

Hypermethylation of the Promoter of miR-338-5p Mediates Aberrant Expression of ETS-1 and Is Correlated With Disease Severity Of Astrocytoma Patients

et al. 2021

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The pro-oncogene ETS-1 (E26 transformation-specific sequence 1) is a key regulator of the proliferation and invasion of cancer cells. The present work examined the correlation of the aberrant expression of ETS-1 with histological or clinical classification of astrocytoma: grade I (pilocytic astrocytoma), grade II (diffuse astrocytoma), grade III (anaplastic astrocytoma), and grade IV (glioblastoma multiforme). MicroRNA, miR-338-5p, was predicted by an online tool (miRDB) to potentially target the 3’ untranslated region of ETS-1; this was confirmed by multi-assays, including western blot experiments or the point mutation of the targeting sites of miR-338-5p in ETS-1’s 3’untralation region (3’UTR). The expression of miR-338-5p was negatively associated with that of ETS-1 in astrocytoma, and deficiency of miR-338-5p would mediate aberrant expression of ETS-1 in astrocytoma. Mechanistically, hypermethylation of miR-338-5p by DNA methyltransferase 1 (DNMT1) resulted in repression of miR-338-5p expression and the aberrant expression of ETS-1. Knockdown or deactivation of DNMT1 decreased the methylation rate of the miR-338-5p promoter, increased the expression of miR-338-5p, and repressed the expression of ETS-1 in astrocytoma cell lines U251 and U87. These results indicate that hypermethylation of the miR-338-5p promoter by DNMT1 mediates the aberrant expression of ETS-1 related to disease severity of patients with astrocytoma.

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“…miR-135b is functionally related to triacylglyceride synthesis through modulating the large tumor suppressor gene 2 (LATS2) [84]. Another upstream regulator of LATS2 is miR-497, which can thus inhibit the production of triglycerides and unsaturated fatty acids in the epithelial cells of the mammary glands of cattle [115]. Introducing a miR-16a mimic into bovine mammary epithelial cells, according to Chen et al ( 2019), in turn, disrupts fat metabolism by targeting the large tumor suppressor kinase 1 (LATS1) [116].…”

Section: Lipid Metabolismmentioning

confidence: 99%

The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep

Dysin

Barkova

Pozovnikova

2021

ncRNA

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Milk is an integral and therefore complex structural element of mammalian nutrition. Therefore, it is simple to conclude that lactation, the process of producing milk, is as complex as the mammary gland, the organ responsible for this biochemical activity. Nutrition, genetics, epigenetics, disease pathogens, climatic conditions, and other environmental variables all impact breast productivity. In the last decade, the number of studies devoted to epigenetics has increased dramatically. Reports are increasingly describing the direct participation of microRNAs (miRNAs), small noncoding RNAs that regulate gene expression post-transcriptionally, in the regulation of mammary gland development and function. This paper presents a summary of the current state of knowledge about the roles of miRNAs in mammary gland development, health, and functions, particularly during lactation. The significance of miRNAs in signaling pathways, cellular proliferation, and the lipid metabolism in agricultural ruminants, which are crucial in light of their role in the nutrition of humans as consumers of dairy products, is discussed.

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miR-497 regulates fatty acid synthesis via LATS2 in bovine mammary epithelial cells

Abstract: Both mRNA and miRNA play an important role in the regulation of mammary fatty acid metabolism and milk fat synthesis. Although studies have shown a strong transcriptional control of fatty...

Cited by 22 publications

References 34 publications

Circ11103 Interacts with miR-128/PPARGC1A to Regulate Milk Fat Metabolism in Dairy Cows

Circ11103 Interacts with miR-128/PPARGC1A to Regulate Milk Fat Metabolism in Dairy Cows

Hypermethylation of the Promoter of miR-338-5p Mediates Aberrant Expression of ETS-1 and Is Correlated With Disease Severity Of Astrocytoma Patients

The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep

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