Circ11103 Interacts with miR-128/<i>PPARGC1A</i> to Regulate Milk Fat Metabolism in Dairy Cows

Chen, Zhi; Lu, Qinyue; Liang, Yusheng; Cui, Xiang-Shun; Wang, Xiaolong; Mao, Yongjiang; Yang, Zhangping

doi:10.1021/acs.jafc.0c07018

Cited by 31 publications

(34 citation statements)

References 41 publications

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“…Fatty acids (FAs) can be divided into four types according to the length of the carbon chain, namely short-chain fatty acids (containing 2-4 carbon atoms, SCFAs), medium-chain fatty acids (containing 6-12 carbon atoms, MCFAs), long-chain fatty acids (containing 13-18 carbon atoms, LCFAs) and very long-chain fatty acids (containing 20 or more carbon atoms, VLCFAs) [1]. In this review, we will refer to VLCFAs, including C20:0, C20:1, C22:0, and others.…”

Section: Introductionmentioning

confidence: 99%

The Overlooked Transformation Mechanisms of VLCFAs: Peroxisomal β-Oxidation

Zong

Zhang

et al. 2022

Agriculture

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Beta-oxidation(β-oxidation) is an important metabolic process involving multiple steps by which fatty acid molecules are broken down to produce energy. The very long-chain fatty acids (VLCFAs), a type of fatty acid (FA), are usually highly toxic when free in vivo, and their oxidative metabolism depends on the peroxisomal β-oxidation. For a long time, although β-oxidation takes place in both mitochondria and peroxisomes, most studies have been keen to explore the mechanism of β-oxidation in mitochondria while ignoring the importance of peroxisomal β-oxidation. However, current studies indicate that it is hard to provide effective treatment for diseases caused by the disorder of peroxisomal β-oxidation, such as X-ALD, SCOX deficiency, and D-BP deficiency; thus, actions should be taken to solve this problem. Based on existing research results, this review will summarize the importance of peroxisomal β-oxidation and help further learning.

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Section: Introductionmentioning

confidence: 99%

The Overlooked Transformation Mechanisms of VLCFAs: Peroxisomal β-Oxidation

Zong

Zhang

et al. 2022

Agriculture

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“…With regard to livestock and poultry, some circRNAs have also been identified by high throughput sequencing and confirmed to participate in regulating their important biological process, such as embryos development ( Veno et al, 2015 ), formation of skeletal muscle fiber ( Li et al, 2020b ), hepatic lipid metabolism ( Huang et al, 2018 ), and adipogenic differentiation ( Li A. et al, 2018 ) in pig; muscle growth and development ( Wei et al, 2017 ; Li et al, 2018b ; Li et al, 2018c ; Liu et al, 2020 ; Shen et al, 2020 ; Yue et al, 2020 ; Elnour et al, 2021 ), testis development ( Gao et al, 2018 ), adipogenesis ( Jiang et al, 2020 ) and milk fat content ( Chen et al, 2021 ) in cattle; fat deposition in buffalo and yak ( Huang et al, 2019 ; Wang H. et al, 2020 ); intramuscular fat (IMF) content denoting a crucial indicator of meat quality in donkey ( Li et al, 2020a ); follicular development ( Shen et al, 2019 ), Marek’s tumourigenesis ( Wang L. et al, 2020 ) as well as muscle growth development ( Ouyang et al, 2018a ; Ouyang et al, 2018b ) in chicken. And it has yet indicated some functional circRNAs related to lipid metabolism, for instance, circFUT10 could promote bovine preadipocyte proliferation but inhibit adipocyte differentiation ( Jiang et al, 2020 ); circRNA_26852 and circRNA_11897, might get involved in porcine adipocyte differentiation and lipid metabolism ( Li A. et al, 2018 ); circ11103 could increase the triglyceride levels in bovine mammary epithelial cells and the contents of unsaturated fatty acids ( Chen et al, 2021 ); additionally, 19:45387150|45389986 and 21:6969877|69753491, were deemed as potential modulators of buffalo back subcutaneous adipose deposition ( Huang et al, 2019 ). These evidences intensively reveal an indispensable role of circRNA in lipid metabolism in livestock.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)

Tian

Zhang

Zhong

et al. 2021

Front. Cell Dev. Biol.

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Circular RNA (circRNA), as a novel endogenous biomolecule, has been emergingly demonstrated to play crucial roles in mammalian lipid metabolism and obesity. However, little is known about their genome-wide identification, expression profile, and function in chicken adipogenesis. In present study, the adipogenic differentiation of chicken abdominal preadipocyte was successfully induced, and the regulatory functional circRNAs in chicken adipogenesis were identified from abdominal adipocytes at different differentiation stages using Ribo-Zero RNA-seq. A total of 1,068 circRNA candidates were identified and mostly derived from exons. Of these, 111 differentially expressed circRNAs (DE-circRNAs) were detected, characterized by stage-specific expression, and enriched in several lipid-related pathways, such as Hippo signaling pathway, mTOR signaling pathway. Through weighted gene co-expression network analyses (WGCNA) and K-means clustering analyses, two DE-circRNAs, Z:35565770|35568133 and Z:54674624|54755962, were identified as candidate regulatory circRNAs in chicken adipogenic differentiation. Z:35565770|35568133 might compete splicing with its parental gene, ABHD17B, owing to its strictly negative co-expression. We also constructed competing endogenous RNA (ceRNA) network based on DE-circRNA, DE-miRNA, DE-mRNAs, revealing that Z:54674624|54755962 might function as a ceRNA to regulate chicken adipogenic differentiation through the gga-miR-1635-AHR2/IRF1/MGAT3/ABCA1/AADAC and/or the novel_miR_232-STAT5A axis. Translation activity analysis showed that Z:35565770|35568133 and Z:54674624|54755962 have no protein-coding potential. These findings provide valuable evidence for a better understanding of the specific functions and molecular mechanisms of circRNAs underlying avian adipogenesis.

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“…The circ_140 inhibited casein secretion and lipid formation in goat mammary epithelial cells ( 24 ). The circ_11103 increased the contents of triglycerides and unsaturated fatty acids in bovine mammary epithelial cells ( 25 ). In this study, we first confirmed the authenticity of circ_015343 by using RT-PCR and Sanger sequencing.…”

Section: Discussionmentioning

confidence: 99%

Tissue-Specific Expression of Circ_015343 and Its Inhibitory Effect on Mammary Epithelial Cells in Sheep

Zhen

Liu

et al. 2022

Front. Vet. Sci.

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Circular RNAs (circRNAs) are a kind of non-coding RNA that have an important molecular function in mammary gland development and lactation of mammals. In our previous study, circ_015343 was found to be highly expressed in the ovine mammary gland tissue at the peak-lactation period by using RNA sequencing (RNA-seq). In the present study, the authenticity of circ_015343 was confirmed by using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and Sanger sequencing. The circ_015343 was derived from the complete 10 exons of aminoadipic semialdehyde synthase (AASS), ranging from exon 2 to exon 11 and mainly located in cytoplasm of ovine mammary epithelial cells. The circRNA was found to be expressed in eight ovine tissues, with the highest expression level in the mammary gland and the least expression in Longissimus dorsi muscle. The circ_015343 had a lower level of expression in a sheep breed with higher milk yield and milk fat content. The disturbed circ_015343 increased the viability and proliferation of the ovine mammary epithelial cells. The inhibition of circ_015343 also increased the expression levels of three milk fat synthesis marker genes: acetyl-coenzyme A carboxylase alpha (ACACA), fatty acid-binding protein 4 (FABP4), and sterol regulatory element-binding protein 1 (SREBP1), as well as three proliferation-related genes: cyclin dependent kinase 2 (CDK2), cyclin dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA), but decreased the expression level of its parent gene AASS. A circRNA-miRNA-mRNA interaction network showed that circ_015343 would bind some microRNAs (miRNAs) to regulate the expression of functional genes related to the development of mammary gland and lactation. This study contributes to a better understanding of the roles of circ_015343 in the mammary gland of sheep.

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Circ11103 Interacts with miR-128/PPARGC1A to Regulate Milk Fat Metabolism in Dairy Cows

Cited by 31 publications

References 41 publications

The Overlooked Transformation Mechanisms of VLCFAs: Peroxisomal β-Oxidation

The Overlooked Transformation Mechanisms of VLCFAs: Peroxisomal β-Oxidation

Dynamic Expression and Regulatory Network of Circular RNA for Abdominal Preadipocytes Differentiation in Chicken (Gallus gallus)

Tissue-Specific Expression of Circ_015343 and Its Inhibitory Effect on Mammary Epithelial Cells in Sheep

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