Lactobacillus pentosusvar.plantarumC29 protects scopolamine-induced memory deficit in mice

Abstract

Aims: In the preliminary study, kimchi, a traditional food fermented with Chinese cabbage, protected scopolamine-induced mouse memory deficit in passive avoidance test. Therefore, we screened protective ingredients, particularly lactic acid bacteria, from Chinese cabbage kimchi against scopolamine-induced memory deficit in mice. Methods and Results: Lactic acid bacteria, isolated from Chinese cabbage kimchi, were identified by 16S rDNA sequence analysis, G+C content and cellular fatty acid composition and sugar fermentation test. Memory deficit was induced in mice by intraperitoneally injecting with scopolamine. Kimchi, particularly its supernatant, protected scopolamine-induced memory deficit in mice in passive avoidance test. Of kimchi ingredients, a lactic acid bacterium, strain C29, potently protected scopolamine-induced memory deficit in mice. C29 was a gram-positive, catalase-negative, anaerobic and non-motile rod. Its pylogenetic property was near to Lactobacillus pentosus (99%) and Lact. plantarum (99%). However, C29 fermented inulin and L-rhamnose and grew in pH 3 and at 45°C in contrast with Lact. pentosus and Lact. plantarum. Therefore, it named to be Lact pentosus var. plantarum C29. The strain C29 protected scopolamine-induced memory deficit in Y-maze and Morris water maze tests. Furthermore, C29 increased hippocampal BDNF and p-CREB expressions, which were reduced by scopolamine. Conclusion: Lactobacillus pentosus var. plantarum C29 may protect memory deficit by inducing BDNF and p-CREB expressions. Significance and Impact of the Study: Lactic acid bacteria, such as Lact pentosus var. plantarum C29, may prevent memory deficit and its contained fermented foods may be beneficial for dementia.

Probiotics: An Adjuvant therapy for D-Galactose induced Alzheimer's disease

“…Recent studies have found that the L. plantarum versus pentosus showed a protective effect against memory deficit in AD-induced mice by D-Galactose and scopolamine [12,18]. Further, another study reported that L. Plantarum NDC75017 improves the learning and memory ability in aging rats [19].…”

Lactobacillus pentosus var. plantarum C29 increases the protective effect of soybean against scopolamine-induced memory impairment in mice

“…Acquisition and retention of the passive avoidance task was conducted using a two-compartment acrylic box wherein a lighted compartment (20 cm  20 cm  20 cm) was connected to a dark compartment (20 cm  20 cm  20 cm) by an entrance door (5 cm  5 cm), like previously reported (Jung et al, 2013). Memory impairment was induced by the intraperitoneal injection of scopolamine (1 mg/kg) 30 min after the oral administrations of test agents.…”

“…The number of arm entries was served as the indicator of locomotor activity. The Morris water maze task was performed, like the previously reported (Jung et al, 2013). Test agents [SE (100 mg/kg), FSE (100 mg/kg), tacrine (10 mg/kg) and vehicle (2% Tween-80, the scopolamine group)] were administered orally into mice 1 h before test was performed at every consecutive day.…”

“…plantarum C29 (KCCM10885, Korea Culture Center of Microorganisms, Seoul, Korea) was cultured at a tryptic soy broth for 20 h at 37 C, collected and used in in vivo study, as previously described (Jung et al, 2013).…”

“…Y-maze was used to evaluate the immediate spatial working memory, as previously described (Jung et al, 2013). Memory impairment was induced by the intraperitoneal injection of scopolamine (1 mg/kg).…”