Lactobacillus pentosus var. plantarum C29 increases the protective effect of soybean against scopolamine-induced memory impairment in mice

Abstract

Biological activities of soybean saponins are dependent on their metabolism by gut microbiota, which generate absorbable bioactive metabolites. Therefore, to enhance the pharmacological effect of soybean, we fermented defatted soybean powder (SP) with Lactobacillus pentosus var. plantarum C29 and measured its protective effect against scopolamine-induced memory impairment in mice using the passive avoidance, Y-maze and Morris water maze tasks. Fermentation increased soyasapogenol B, genistein and daidzein content of soybean and enhanced the protective effect of soybean against scopolamine-induced memory impairment. Additionally, compared with the exthanol extract of soybean, fermented SP (FSP) increased the expression of brain-derived neurotrophic factor (BDNF) in the hippocampi of scopolamine-treated mice. Furthermore, FSP inhibited acetylcholinesterase (AChE) activity in vitro and ex vivo. These findings suggest that C29 fermentation might increase the ameliorating effect of soybean against memory impairments by inhibiting AChE activity and increasing BDNF expression.

Lactobacillus plantarum C29‐Fermented Soybean (DW2009) Alleviates Memory Impairment in 5XFAD Transgenic Mice by Regulating Microglia Activation and Gut Microbiota Composition

“…Lactobacillus plantarum C29 increased cognitive function in aged rats and attenuated LPS‐ or scopolamine‐induced memory impairment in mice . C29‐fermented defatted soybean powder (FDS, DW2009) also inhibited gut microbiota LPS production and attenuated LPS‐ or scopolamine‐induced memory impairment in mice . Nevertheless, the memory impairment‐ameliorating mechanism of FDS against AD remains elusive.…”

Efficacy and Safety of Lactobacillus Plantarum C29-Fermented Soybean (DW2009) in Individuals with Mild Cognitive Impairment: A 12-Week, Multi-Center, Randomized, Double-Blind, Placebo-Controlled Clinical Trial

“…Based on the results from our previous animal study that showed cognitive enhancement effects of DW2009 and considering the acceptable daily intake of Lactobacillus genus for humans without appreciable adverse effects [16], the dose of 800 mg/day was selected. DW2009 is a mixture of fermented soybean powder and Lactobacillus plantarum C29 freeze-dried powder.…”

A Review of Fermented Foods with Beneficial Effects on Brain and Cognitive Function

“…Similarly, fermented soybean powder with Lactobacillus pentosus var. plantarum C29 showed a protective effect against scopolamine-induced memory impairment in mice (31). Scopolamine-induced reduction of hippocampal BDNF expression was reversed, and AChE activity was inhibited after treatment with fermented soybean powder extract (31).…”

“…plantarum C29 showed a protective effect against scopolamine-induced memory impairment in mice (31). Scopolamine-induced reduction of hippocampal BDNF expression was reversed, and AChE activity was inhibited after treatment with fermented soybean powder extract (31). A cross-sectional clinical study also reported that fermented soybean products oriented diets have a beneficial effect among this particular population.…”