Lactobacillus bulgaricusmutants decompose uremic toxins

Abstract

Background/Aims: We aim to obtain a probiotic strain from Lactobacillus bulgaricus by testing its capability to decompose uremic toxins to provide new intestinal bacteria for the treatment of chronic renal failure. Methods: Original L. bulgaricus was cultured with the serum of uremic patients and then mutated by physical (ultraviolet) and chemical (diethyl sulfate) methods repeatedly. Using creatinine decomposition rate as an observed index, we selected the best strains which decreased the most concentration of the creatinine. We then tested its ability to decompose urea, uric acid, serum phosphate, parathyroid hormone, and homocysteine and its genetic stability. Results: After inductive and mutagenic treatment, DUC3-17 was selected. Its decomposition rate of creatinine, urea nitrogen, uric acid, phosphorus, parathyroid hormone, and homocysteine were 17.23%, 36.02%, 9.84%, 15.73%, 78.26%, and 12.69%, respectively. The degrading capacity was sustained over five generations. Conclusions: After directional induction and compound mutation, L. bulgaricus has greater capacity to decompose uremic toxins, with a stable inheritance.

The potential of probiotics in the amelioration of hyperuricemia

“…Additional progress has been made in these areas, such as in the use of probiotics including Lactobacillus and Bifidobacterium , which can reduce inflammation and hyperuricemia in mouse models for unclear reasons. 23,160–162 The probiotics for exploration as possible treatments for hyperuricemia are listed in Table 1.…”

Research Progress of Natural Active Substances with Uric-Acid-Reducing Activity

“…In one study, original Lactobacillus bulgaricus was cultured with the serum of uremic patients before being subjected to directional induction and compound mutation to produce L. bulgaricus DUC3-17, which can effectively decompose UA and uremic toxins . As pointed out by Chen et al, Aspergillus MFE-120 was a kind of high uricase activity from Aspergillus.…”

Probiotics and CKD Progression: Are Creatinine-Based Estimates of GFR Applicable?

“…Such determinations may be singularly inaccurate because probiotics can decrease the creatinine concentration by altering the gut microbiome, thereby increasing the extrarenal elimination of creatinine. 3 When creatinine concentration is reduced by probiotic use without affecting GFR, the efficacy of probiotics to preserve kidney function in those with CKD may be overestimated.…”

“…4 Large numbers of gut bacteria with creatininase activity explain why creatinine-based estimated GFR (eGFR) is often inaccurately high in CKD 5 and why the use of probiotics, if containing organisms with the ability to eliminate creatinine, can result in reductions in plasma creatinine levels. 5 Creatininase activity has been demonstrated in bacterial species used in probiotic preparations, 3,6 as well as many other organisms, 7 including an isolate from an infected ileal loop urine. 8 Antibiotics alter gut microbiota when consumed, 9 and a lack of creatinine degradation has been reported in stool samples of uremic patients who recently received antibiotics.…”