In vivo imaging and biodistribution of near infrared dye loaded brain-metastatic-breast-cancer-cell-membrane coated polymeric nanoparticles

Abstract

Brain metastatic breast cancer is challenging to treat due to the presence of the blood-brain barrier (BBB) and a lack of ability to target precisely. Most drugs fail to cross the BBB limiting their effectiveness. To combat this problem, a brain metastatic breast cancer cell (MDA-MB-831) membrane-coated polymeric nanoparticle (CCNP) was synthesized. The small size (∼70 nm) and anionic surface charge (−20 mV) achieved during formulation allowed for high penetration and retention in the brain when compared to the PEGylated polymeric nanoparticle alone (mPEG-PLGA or NP). Doxorubicin-loaded CCNP showed high preferential cytotoxicity in vitro. Live (4-120 h) and ex vivo near-infrared imaging in nude mice showed extended circulation and retention of CCNP compared to uncoated nanoparticles. These data indicate that drug/dye-loaded CCNPs demonstrate excellent potential for cancer theranostics of brain metastatic breast tumors.

PLGA protein nanocarriers with tailor-made fluorescence/MRI/PET imaging modalities

“…Such preclinical in vitro and in vivo studies, comprising multiple biological characterization phases, could be expedited by endowing the carrier with complementary imaging capacities. For instance, in vitro cellular uptake can be easily imaged with fluorescence 13,14 while radioimaging 15,16 or near infrared (NIR) imaging 1,17 are well suited for in vivo biodistribution studies. However, the simultaneous incorporation of all these imaging probes into a single nanocarrier unnecessarily increases the complexity of the system, potentially limiting translation to the clinics.…”

“…In all cases, the imaging moieties are chemically attached to the PLGA shell matrix as opposed to being entrapped in the core of the nanocapsule. [13][14][15]17 This approach is advantageous to avoid interferences of the imaging moieties with the cargo in the core. 21 This is especially important for delicate payloads such as proteins, enzymes or microRNAs.…”

Camouflaging Nanoparticles with Brain Metastatic Tumor Cell Membranes: A New Strategy to Traverse Blood–Brain Barrier for Imaging and Therapy of Brain Tumors

“…One recent work was reported by Kumar et al, in which authors used breast cancer cell membrane to coat PEGylated poly(lactic‐co‐glycolic acid) particles for long term near‐infrared (NIR) imaging in nude mice and studied their distribution in the brain. [ 25 ] Inspired by this, we herein propose a new strategy to construct a brain metastatic tumor cell membrane‐based biomimetic nanocarrier, which is able to cross BBB for imaging and photothermal therapy (PTT) for early brain tumors ( Scheme ). The biomimetic nanocarrier with core–shell structure is constructed with two components.…”

Biomimetic Nanoparticles Camouflaged in Cancer Cell Membranes and Their Applications in Cancer Theranostics

“…Cloaking cancer cell membranes onto upconversion nanoparticles (UCNPs) to obtain CC-UCNPs have been reported (26). In another study, a brain metastatic breast cancer cell (MDA-MB-831) membrane-coated polymeric nanoparticle (mPEG-PLGA) platform was constructed (21). NIR dye IR780 was loaded into the mPEG-PLGA polymeric NPs for imaging.…”

“…The RBC membrane was found to act as a nanosponge for toxins, and bestowed a longer circulation pharmacokinetic profile than uncoated NPs (3). Since this initial study, cell membrane coating technology has significantly expanded to the use of membranes from platelets (4)(5)(6)(7)(8) and from nucleated cells, such as macrophages (9)(10)(11)(12), neutrophils (13), beta cells (14), and cancer cells (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33). The use of cancer cell plasma membranes has attracted attention because these membranes carry tumor-specific receptors and antigens that play a role in cancer cell proliferation, invasion, and metastasis.…”