<i>In vitro</i>studies on somaclonal variation and induced mutagenesis: progress and prospects in sugarcane (<i>Saccharum</i>spp.) – a review

Rutherford, R. S.; Snyman, S. J.; Watt, M. P.

doi:10.1080/14620316.2014.11513041

Cited by 11 publications

(5 citation statements)

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“…RAPD-PCR is the most commonly used marker to detect polymorphism and variability (Shahid et al, 2012; Sobhakumari, 2012). It is easy, quick, and requires small quantity of template DNA.…”

Section: Resultsmentioning

confidence: 99%

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

2018

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Lipases are enzymes that have the potential to hydrolyze triacylglycerol to free fatty acids and glycerol and have various applications. The aim of the present study was to isolate and screen marine bacteria for lipase production, optimize the production, and treat wastewater. A total of 20 marine bacterial isolates were obtained from the Mediterranean Sea and were screened for lipase production. All isolates were found to have lipolytic ability. The differences between the isolates were studied using RAPD-PCR. The most promising lipase producer (isolate 3) that exhibited the highest lipolytic hydrolysis (20 mm) was identified as Bacillus cereus HSS using 16S rDNA analysis and had the accession number MF581790. Optimization of lipase production was carried out using the Plackett–Burman experimental design with cotton seed oil as the inducer under shaking conditions at 10°C. The most significant factors that affected lipase production were FeSO4, KCl, and oil concentrations. By using the optimized culture conditions, the lipase activity increased by 1.8-fold compared with basal conditions. Immobilization by adsorption of cells on sponge and recycling raised lipase activity by 2.8-fold compared with free cells. The repeated reuse of the immobilized B. cereus HSS maintained reasonable lipase activity. A trial for the economic treatment of oily wastewater was carried out. Removal efficiencies of biological oxygen demand, total suspended solids, and oil and grease were 87.63, 90, and 94.7%, respectively, which is promising for future applications.

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“…RAPD-PCR is the most commonly used marker to detect polymorphism and variability (Shahid et al, 2012; Sobhakumari, 2012). It is easy, quick, and requires small quantity of template DNA.…”

Section: Resultsmentioning

confidence: 99%

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

2018

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“…Higher concentration of SA (0.3%) and increased treatment time period (60 min) have more influence on the number of days for shoot formation. As represented in Table 6, interaction effect of both the factors displayed significant, in which calli treated with C [7] , Melion and Barba (1980) [15] , and Rutherford et al (2013) [26] . Genetic nature of CoN -13073 did not respond well to mutagenic treatments for this character.…”

Section: Effect Of Different Chemical Mutagenic Agents On the Number Of Days For Shoot Formationmentioning

confidence: 99%

Genetic variability analysis in sugarcane (Saccharum spp. complex) through in vitro chemical mutagenesis on callus culture

AJ¹,

VL²,

Acharya³

et al. 2021

Pharma Innovation

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The present investigation on was carried out in south Gujarat heavy rainfall region of India. Callus derived from sugarcane variety CoN -13073 subjected for mutagenic treatment varying in different concentration as well as time period. Callus culture of 25 -30 days old white globular form is considered a suitable material to undergo mutagenic treatments. Among the mutagenic treated calli, maximum survival per cent was observed in EMS 0.2% + 60 min treatment. Maximum regeneration per cent (78.2%) was found in explants treated with treatment MMS 0.3% + 30 min. In case of the number of days for shoot formation, callus treated with EMS 0.2% + 60 min registered a maximum of 15.20 days. The maximum numbers of multiple shoots were observed in MMS 0.2% + 60 min (30.4). Time factor found to be highly significant with a wide range of mean performance. The effect of the mutagenic agent was found to be succeeding to create variability at a lower concentration for lower time intervals for most of the characters under study. In the present study EMS, 0.2% in addition to 0.3%, treatments were most effective. And the effectiveness decrease with the increase in the time interval.

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“…En México no existe información sobre mutagénesis inducida con radiación ionizante en yemas de caña de azúcar. En general, los trabajos de radiación en caña de azúcar con radiación ionizante in vitro se centraron en determinar dosis y tiempos de exposición para producir el mayor número de mutaciones, sin causar la muerte (Rutherford et al, 2014). Algunas características estudiadas son: selección para salinidad, sequía y caracterización de los mutantes seleccionados vía mutagénesis in vitro (Patade et al, 2006;Suprasanna et al, 2009), resistencia a la podredumbre roja, anegamiento y retraso en la floración (Majid et al, 2001;Samad et al, 2001), tolerancia a la salinidad (Saif et al, 2001), para mejorar el diámetro del tallo (García et al, 2001), estrés por sequía (Hartati et al, 2022), para resistencia a diferentes enfermedades como pudrición roja (Rao et al, 1966;Ali et al, 2007), virus del mosaico de la caña de azúcar (Fuchs et al, 2002), carbón (Nasiru and Ifenkwe, 2004), roya (Valdez et al, 2004;Oloriz et al, 2012) y Rutherford et al (2014) al realizar una revisión mencionan diferentes características agronómicas como el peso y rendimiento de la caña, el número de macollos, diámetro del tallo, altura del tallo, entre otras, obtenido de callos, meristemo apical y brotes de caña irradiados.…”

Section: Introductionunclassified

Dosis altas de radiación gamma (Cobalto60) disminuyen la expresión fenotípica de caracteres en caña de azúcar (Saccharum officinarum L.)

Díaz-Juárez¹,

Hernández-Arenas²,

Santacruz-Várela³

et al. 2022

Braz. J. Anim. Environ. Res.

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La mutagénesis inducida es una técnica para crear variabilidad genética de manera rápida, directa y fácil en plantas de propagación vegetativa. A través de un agente mutagénico se provocan cambios en una o varias características morfológicas y fisiológicas que se transmiten a su progenie. En México no existe información sobre mutagénesis inducida en el cultivo de caña de azúcar. Por tal motivo, el objetivo del trabajo fue determinar el efecto de la radiación gamma a base de Cobalto60 en la expresión de caracteres fenotípicos en dos variedades comerciales de caña de azúcar. Fueron irradiadas 100 yemas de cada variedad, CP 72-2086 y MEX 69-290, con seis diferentes dosis de radiación gamma: 0, 50, 70, 80, 90 y 100 Grey con Cobalto60. Se sembraron en maceta bajo malla sombra y después se trasplantaron a campo. Los caracteres evaluados fueron emergencia de yemas, caracteres morfológicos y componentes de rendimiento. En general, a mayor dosis de radiación gamma, mayor afectación en la expresión fenotípica de las variables evaluadas, resultando diferentes interacciones entre los factores involucrados. Las dosis de 90 y 100 Grey causaron mayor efecto en la disminución de la expresión fenotípica de los caracteres y demoraron el crecimiento de las plantas. En las interacciones, la variedad CP 72-2086 fue más sensible en la emergencia de yemas, altura de planta, número de macollos y longitud del entrenudo, y la MEX 69-290 en área foliar, los que nos indica que, existe diferente grado de sensibilidad en los caracteres y las variedades evaluadas. Por lo tanto, las dosis altas de radiación gamma causan disminución en diferente proporción en la expresión fenotípica de caracteres y demora el crecimiento de las plantas en caña de azúcar. De igual manera, existe diferente grado de sensibilidad a la radiación gamma en los caracteres y genotipos.

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In vitrostudies on somaclonal variation and induced mutagenesis: progress and prospects in sugarcane (Saccharumspp.) – a review

Cited by 11 publications

References 1 publication

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Production of Cold-Active Lipase by Free and Immobilized Marine Bacillus cereus HSS: Application in Wastewater Treatment

Genetic variability analysis in sugarcane (Saccharum spp. complex) through in vitro chemical mutagenesis on callus culture

Dosis altas de radiación gamma (Cobalto60) disminuyen la expresión fenotípica de caracteres en caña de azúcar (Saccharum officinarum L.)

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