The present investigation on was carried out in south Gujarat heavy rainfall region of India. Callus derived from sugarcane variety CoN -13073 subjected for mutagenic treatment varying in different concentration as well as time period. Callus culture of 25 -30 days old white globular form is considered a suitable material to undergo mutagenic treatments. Among the mutagenic treated calli, maximum survival per cent was observed in EMS 0.2% + 60 min treatment. Maximum regeneration per cent (78.2%) was found in explants treated with treatment MMS 0.3% + 30 min. In case of the number of days for shoot formation, callus treated with EMS 0.2% + 60 min registered a maximum of 15.20 days. The maximum numbers of multiple shoots were observed in MMS 0.2% + 60 min (30.4). Time factor found to be highly significant with a wide range of mean performance. The effect of the mutagenic agent was found to be succeeding to create variability at a lower concentration for lower time intervals for most of the characters under study. In the present study EMS, 0.2% in addition to 0.3%, treatments were most effective. And the effectiveness decrease with the increase in the time interval.
Callus derived from sugarcane variety CoN -13073 subjected for mutagenic treatment varying in different concentration as well as time period. Callus culture of 25 -30 days old white globular form is considered a suitable material to undergo mutagenic treatments. Among the mutagenic treated calli, Maximum shoot length (26.6 cm) was observed in the treatment, EMS 0.2% + 30 min. Maximum root length (12.8 cm) was observed in treatment MMS 0.3% + 30 min. A higher concentration of all mutagenic agents registered higher root length with the maximum of 1.14 g was observed in treatment EMS 0.3% + 60 min. Maximum leaf area (44.7 cm 2 /plant) was observed in treatment MMS 0.2% + 30 min. Irrespective to concentration SA and EMS found significant for this character. Maximum chlorophyll content index (4.87) was observed in treatment EMS 0.2% + 30 min. In case of this character higher to lower concentration as well as time period influenced in both higher and lower directions. Among the treatments, the maximum multiplication rate (12.45%) was observed in treatment EMS 0.2% + 30 min. Both factors showed different effects on this character. The effect of the mutagenic agent was found to be succeeding to create variability at a lower concentration for lower time intervals for most of the characters under study. In the present study EMS, 0.2% in addition to 0.3%, treatments were most effective. And the effectiveness decreases with the increase in the time interval.
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