In vitroselection of a synbiotic andin vivoevaluation on intestinal microbiota, performance and physiological response of rainbow trout (Oncorhynchus mykiss) fingerlings

Abstract

The aim of this study was to determine the best synbiotic combination (based on growth and short chain fatty acids production) between Pediococcus acidilactici and five prebiotics in vitro, inulin, fructooligosaccharide (FOS), xylooligosaccharide (XOS), galactooligosaccharide (GOS), isomaltooligosaccharide (IMO). The best in vitro growth under aerobic and anaerobic culture condition was observed in the treatment, P. acidilactici + GOS. Furthermore, the study of short chain fatty acids (SCFA) production at the end of exponential growth phase revealed that the major SCFA produced was propionic acid in all synbiotic treatments except for the P. acidilactici + GOS treatment where butyric acid production was significantly higher compared with the other treatments. Based on the results of in vitro studies, P. acidilactici + GOS that were selected as synbiotic and rainbow trout fingerlings (15.04 ± 0.52 g) were fed on diet supplemented with the selected synbiotic. After 8 weeks of feeding, growth performance, intestinal microbiota and physiological responses were evaluated. Growth performance was significantly improved by synbiotic feeding. No significant differences of haematological parameters were revealed between the different treatments. Level of presumptive autochthonous lactic acid bacteria was elevated, while total autochthonous bacterial level remained unaffected. These results showed that P. acidilactici + GOS can be considered as an efficient synbiotic for rainbow trout.

The effects of galactooligosaccharide on systemic and mucosal immune response, growth performance and appetite related gene transcript in goldfish (Carassius auratus gibelio)

“…The literature review revealed contradictory results regarding the effect of GOS on fish growth performance and feed efficiency. Though, there are some reports confirmed improvement of growth performance in GOS-fed red drum [12,16], Caspian roach fry [10], this prebiotic had no significant effects on performance of rainbow trout [35], Hybrid Striped Bass (Morone chrysops × Morone saxatilis) [13]. These differences can be attributed to species, life stage, dosage and experimental condition and highlighted the necessity of evaluation of different prebiotics on desired species.…”

Effects of synbiotic containing Lactobacillus plantarum 7-40 and galactooligosaccharide on the growth performance of white shrimp, Litopenaeus vannamei

“…(); Hoseinifar, Mirvaghefi et al. (). Lactobacillus plantarum 7–40 was first cultured in MRS broth for 24 hr at 37°C, then centrifuged at 3,000 × g for 10 min at 4°C.…”

“…Synbiotics, a combination of prebiotics and probiotics, have been used since 2005 (Panigrahi et al., ). Synbiotics have been widely applied and are assumed to be growth‐ and immunity‐promoting factors in freshwater fish (Azimirad, Meshkini, Ahmadifard, & Hoseinifar, ; Hoseinifar, Dadar, & Ringø, ; Hoseinifar, Esteban, Cuesta, & Sun, ; Hoseinifar, Mirvaghefi, Amoozegar, Merrifield, & Ringø, ) and marine fish (Ai et al., ; Reyes‐Becerril et al., ). It is surprising that although growth‐enhancing effect of synbiotics on the growth of fish is well documented, little information was reported in cultured Penaeid shrimp.…”

“…Fructooligosaccharide (FOS), galactooligosaccharide (GOS) and mannan oligosaccharide (MOS) are commonly used prebiotics and were recently applied in aquaculture as additives (reviewed by Ringø et al., ). These compounds have also been used as parts of synbiotics to improve the growth of fish (Hoseinifar, Hoseini, & Bagheri, ; Hoseinifar, Mirvaghefi et al., ; Modanloo, Soltanian, Akhlaghi, & Hoseinifar, ; Rodriguez‐Estrada, Satoh, Haga, Fushimi, & Sweetman, ; Zhang et al., ). However, their effects of improving the growth of L. vannamei remain a subject of intense debate.…”

Growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus fed with fructooligosaccharide

“…Non‐digestible oligosaccharides such as FOS can be consumed as substrate by potentially beneficial microbial community and increase their number and dominance (Ringø et al, ) either by their presence or through fermentation and production of health‐promoting substance such as SCFAs (Hoseinifar, Sun, et al, ). From the few available works, the main valuable effects reported for those prebiotics in fish were improvements of growth performance, digestibility and immunity (Guerreiro, Serra, Oliva‐Teles, & Enes, ; Hoseinifar, Mirvaghefi, Amoozegar, Ringø, & Merrifield, ). However, few studies were available regarding prebiotics use in this species.…”