<i>In vitro</i>Propagation of<i>Valeriana wallichii</i>

Mathur, Jaideep; Ps, Ahuja; Mathur, Anmol; Ak, Kukreja; Nc, Shah

doi:10.1055/s-2006-962346

Cited by 30 publications

(8 citation statements)

References 5 publications

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“…Synergistic effect of a range of growth regulator combinations on promotion of shoot multiplication is well documented for medicinal plants. Various successful combinations have been reported in various studies such as BA + IAA for Adhatoda beddomei (Sudha and Seeni 1994), Valeriana wallichii (Mathur et al 1987), BA + IBA for Rheum emodi (Lal and Ahuja 1993) and BA + NAA for Gompherena officinalis (Mercier et al 1992) but the increase in the concentration of auxin suppresses the bud differentiation. This may be explained by assuming that the amount of auxin synthesized endogenously was more than sufficient to interact with added cytokinin to achieve the balance, necessary for bud induction.…”

Section: Analysis Of Datamentioning

confidence: 99%

In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India

Bisht

Bhandari

2011

Physiol Mol Biol Plants

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An ideal micropropagation method of Polygonatum verticillatum has been developed using stem disc explants. Multiple shoots were initiated from stem disc explants on Murashige and Skoog (MS) medium fortified with different concentrations (0.25-10.0 mgl NAA was found to be the most effective in producing maximum number of shoots. Regular subculturing of these in vitro multiple shoots induced profuse growth of lateral roots in the same medium. Individual shoots were excised and rooted in vitro on half strength MS medium with 1.0 mglNAA. Regenerants were hardened in growth chamber with high humidity and showed a high rate of survival.(Family-Liliaceae) commonly known as whorled solomon's seal (Meda) is a distinguished medicinal plant of temperate Himalaya. It is distributed frequently as undergrowth in forest of temperate regions between 2,000 and 3,000 m elevations. The rhizome of this plant constitutes an important ingredient of Ashtavarga, a group of eight drugs used extensively in Ayurveda mainly as a tonic and aphrodisiac. It has been reported to pacify pitta dosa, increase cough and shukra and is useful in phthisis, burning sensation in the body, fever and has significant antifungal activity (Chaumont 1979;Varier 1994). In trade, its rhizome is known as Meda/ Mahameda and its medicinal attributes are ascribed mainly to the presence of steroidal saponins and polysaccharides in the rhizome. The plant is also useful in the preparation of cosmetics, skin tonic and as a vegetable. Due to large scale and indiscriminate collection of wild material, P. verticillatum is rapidly disappearing. There is a need for conservation and mass multiplication of this plant. To fill the gap between demand and supply of its tuberous roots for commercial gains and to provide cost-effective planting material for conventional propagation methods at a rapid rate in a short span of time, plant tissue culture approach seems to be a profitable alternative. Materials and methods Plant materialPlants of P. verticillatum were collected from natural habitat (from the forest division in Pauri Garhwal and Mandal in Gopeshwar) and were maintained in earthen pots containing mixture of sand, soil and farmyard manure (1:1:1) v/v. These plants were used as the mother source for obtaining explants for tissue culture experiments. Establishment of aseptic culturesThe young rhizomes were washed thoroughly with 1 % (v/v) Labolene detergent for 15 min in a gentle continuous flow of tap water followed by 0.1 % w/v

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Section: Analysis Of Datamentioning

confidence: 99%

In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India

Bisht

Bhandari

2011

Physiol Mol Biol Plants

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“…Valepotriates also have been shown to have antifungal (Fuzzati et al 1996) and cytotoxic (Bounthanh et al 1981;Bos et al 1998a) activities. As an alternative to obtain these active compounds of pharmaceutical interest, some species have been investigated: V. officinalis, cultivated in Europe on a large scale (Bos et al 1998b); V. wallichii, native to the Himalayas (Mathur et al 1988), and V. edulis originated from Central America (Castillo et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Role of Light and Medium Composition on Growth and Valepotriate Contents in Valeriana glechomifolia Whole Plant Liquid Cultures

Russowski

Maurmann

Rech

et al. 2006

Plant Cell Tiss Organ Cult

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A system for growing in liquid medium whole plants of Valeriana glechomifolia, endemic to southern Brazil and capable of accumulating bioactive valepotriates, is described. Murashige and Skoog (MS) and Gamborg B5 (B5) media (1.0·, 0.3· and 0.1· strength) without phytohormones were evaluated after four weeks of culture in relation to growth and valepotriate yield. Plants grown in 1.0· MS displayed greatest growth and valepotriate yields and the study of the light condition showed that plants grown under light and dark had similar weight increase and maximum valepotriate yield, 27.2 mg/g DW and 25.0 mg/g DW, respectively. Valtrate was the most abundant valepotriate, followed by acevaltrate and didrovaltrate.

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“…The effective response of plantlets to IAA during the micropropagation of other Valerianaceae species has also been reported (Mathur et al 1988;Enciso-Rodríguez 1997).…”

Section: Discussionmentioning

confidence: 91%

Control of development and valepotriate production by auxins in micropropagated Valeriana glechomifolia

et al. 2004

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Valeriana glechomifolia is a plant species endemic to southern Brazil that accumulates valepotriates, which are terpene derivatives, in all of its organs. Valepotriates are the presumed sedative generic components of the pharmaceutically used species of Valeriana. The influence of various concentrations of the auxins indole-3-acetic acid, indole-3-butyric acid and alpha-naphthaleneacetic acid on the growth of micropropagated V. glechomifolia was investigated under conditions of transient and continuous exposure. Changes in the development of roots and shoots as well as the production of the valepotriates acevaltrate, valtrate and didrovaltrate (analyzed by high-performance liquid chromatography) were evaluated. The best performance in valepotriate production, growth and survival under ex vitro conditions following plant acclimatization was achieved in the continuous presence of 5.71 microM IAA. When cultured in medium containing IAA plants produced stable levels of valepotriates throughout the entire cultivation period.

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In vitroPropagation ofValeriana wallichii

Cited by 30 publications

References 5 publications

In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India

In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India

Role of Light and Medium Composition on Growth and Valepotriate Contents in Valeriana glechomifolia Whole Plant Liquid Cultures

Control of development and valepotriate production by auxins in micropropagated Valeriana glechomifolia

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