<i>In vitro</i>immunomodulatory effects of extracts from three plants of the<i>Labiatae</i>family and isolation of the active compound(s)

Amirghofran, Zahra; Hashemzadeh, Reihaneh; Javidnia, Katayoun; Golmoghaddam, Hossein; Esmaeilbeig, Ahmad R.

doi:10.3109/1547691x.2011.590828

Cited by 45 publications

(26 citation statements)

References 22 publications

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“…These glycosides were useful for treating neutrophil function deficiency and chronic granulomatous diseases (Sheng et al, 2003;Solanki and Jain, 2012). Several other studies also support our findings (Amirghofran et al, 2011;Fakeye et al, 2008;Kalaivani and Mathew, 2010;Singh et al, 2011).…”

Section: Delayed-type Hypersensitivity Responsesupporting

confidence: 80%

Immunomodulatory activity of kaempferol 5-O-β-d-glucopyranoside from Indigofera aspalathoides Vahl ex DC. (Papilionaceae)

2015

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The objective of this study was to isolate a new compound from methanol extracts of Indigofera aspalathoides Vahl ex DC. aerial parts and to determine the immunomodulatory activity of the isolated compound. The methanolic extract yielded compounds 1 (0.65 mg), 2 (0.52 mg), and 3 (1.80 g) on chromatography, which was characterized using spectral techniques, namely Fourier transform infrared, 1 H nuclear magnetic resonance, 13 C nuclear magnetic resonance, and mass spectroscopy. Owing to the paucity of compounds 1 and 2, kaempferol 5-O-b-Dglucopyranoside (compound 3) was evaluated for immunomodulatory activity. Compound 3 was assessed in a neutrophil adhesion and a delayed-type hypersensitivity test, and humoral antibody titers were measured in albino rats. Administration of compound 3 at oral doses of 10, 20, 50, and 100 mg/kg of body weight resulted in a significant (p \ 0.05) increase in percent neutrophil adhesion to nylon fibers, a dose-dependent increase in antibody titers, and potentiation of the delayed-type hypersensitivity reaction induced using sheep red blood cells. We concluded that compound 3, methanol extracted from aerial parts of I. aspalathoides Vahl ex DC. possessed significant immunomodulatory activity and therefore has promising therapeutic potential for the prevention of autoimmune diseases.

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Section: Delayed-type Hypersensitivity Responsesupporting

confidence: 80%

Immunomodulatory activity of kaempferol 5-O-β-d-glucopyranoside from Indigofera aspalathoides Vahl ex DC. (Papilionaceae)

2015

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“…Thymol displays antimicrobial [18e20] and wound-healing activity [21] and is able to rise the levels of macrophage migration inhibitory factor (MIF) in central nervous system [22]. Thymol increased the in vitro fibroblast growth [23], effectively inhibited COX-1 [24] and prevented inducible lymphocyte proliferation [25].…”

Section: Introductionmentioning

confidence: 99%

Kinetic and in silico studies of novel hydroxy-based thymol analogues as inhibitors of mushroom tyrosinase

Ashraf

Rafiq

Seo

et al. 2015

European Journal of Medicinal Chemistry

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“…The methanol extract was filtered and then concentrated in a vacuum at 40 C using a rotary evaporator (Amirghofran et al, 2011a). The residue was lyophilized and the resultant powdered material stored at À20 C until tested.…”

Section: Preparation and Fractionation Of The Crude Methanol Extractmentioning

confidence: 99%

Anti-inflammatory effect ofMentha longifoliain lipopolysaccharide-stimulated macrophages: Reduction of nitric oxide production through inhibition of inducible nitric oxide synthase

Karimian

Kavoosi

Amirghofran

2013

Journal of Immunotoxicology

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Mentha longifolia is an aromatic plant used in flavoring and preserving foods and as an antiinflammatory folk medicine remedy. The present study assessed the effects of M. longifolia extracts, including essential oil and crude methanol extract and its fractions (ethyl acetate, butanol and hexane), on nitric oxide (NO) production and inducible NO synthase (iNOS) mRNA expression in lipopolysaccharide (LPS)-stimulated J774A.1 cells using real-time polymerase chain reaction (PCR). The cytotoxic effects of the extracts on the cells were examined and noncytotoxic concentrations (50.2 mg/ml) were used to examine their effects on NO production and iNOS mRNA expression. Only the hexane fraction that contained high levels of phenolic and flavonoid compounds at concentrations from 0.05-0.20 mg/ml significantly reduced NO production in LPS-stimulated cells (p50.001). Real-time PCR analysis indicated the ability of this fraction at the same concentrations to significantly decrease iNOS as well as TNFa mRNA expression in the cells (p50.001). All extracts were able to scavenge NO radicals in a concentration-dependent manner. At concentrations greater than 0.2 mg/ml, total radicals were 100% scavenged. In conclusion, M. longifolia possibly reduces NO secretion in macrophages by scavenging NO and inhibiting iNOS mRNA expression, and also decreases TNFa pro-inflammatory cytokine expression, thus showing its usefulness in the inflammatory disease process. KeywordsInducible nitric oxide synthase (iNOS), macrophages, Mentha longifolia, nitric oxide (NO) History

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In vitroimmunomodulatory effects of extracts from three plants of theLabiataefamily and isolation of the active compound(s)

Cited by 45 publications

References 22 publications

Immunomodulatory activity of kaempferol 5-O-β-d-glucopyranoside from Indigofera aspalathoides Vahl ex DC. (Papilionaceae)

Immunomodulatory activity of kaempferol 5-O-β-d-glucopyranoside from Indigofera aspalathoides Vahl ex DC. (Papilionaceae)

Kinetic and in silico studies of novel hydroxy-based thymol analogues as inhibitors of mushroom tyrosinase

Anti-inflammatory effect ofMentha longifoliain lipopolysaccharide-stimulated macrophages: Reduction of nitric oxide production through inhibition of inducible nitric oxide synthase

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