In vitro biocompatibility of degradable biopolymers in cell line cultures from various ocular tissues: Direct contact studies

Abstract

Synthetic biodegradable polymers have many potential therapeutic applications. In ophthalmology, biodegradable polymers have been used as viscoelastic agents and surgical implants. Other potential applications include controlled release of drugs and growth factors, gene therapy, and tissue engineering. In the present study, in vitro biocompatibility of three biodegradable polymers, 50:50 PDLGA, 85:15 PDLGA, and Inion GTR membrane was evaluated in comparison to tissue culture polystyrene by investigating cell proliferation and potential acute toxicity by the WST-1 cytotoxicity/cell proliferation test, the ATP test, and the lactate dehydrogenase (LDH) test. Evaluations were conducted with cell line cultures from various ocular tissues, human corneal epithelial cells (HCE), rabbit stromal fibroblasts (SIRC), bovine corneal endothelial cells (BCE), human conjunctival epithelial cells (IOBA-NHC), and human retinal pigment epithelial cells (ARPE-19) by direct contact studies by plating the cells on the polymer film specimens in 96-wells. The proliferation results show that cell lines from various ocular tissues attached and grew on PDLGA 50:50, PDLGA 85:15, and Inion GTR membrane. Cytotoxicity experiments with the LDH and ATP tests showed no or extremely slight toxic adverse effects. These polymers have potential to be used as scaffolds in cell transplantation devices or as surgical implants.

The Effects of 5-Fluorouracil on Ocular Tissues In Vitro and In Vivo after Controlled Release from a Multifunctional Implant

“…14 Cell cultures were maintained in conditions similar to those described in our previous studies. 21,22 The cells were seeded at the density of 1 ϫ 10 4 , 1.5 ϫ 10 4 , and 2 ϫ 10 4 cells/well into 96-well microplates for the exposure times of 72, 48, and 24 hours, respectively. Twenty-four hours after plating, medium was discarded and the preconfluent cell cultures were replaced with normal growth medium or with a test solution containing 0.00005 to 5 mg/mL 5-FU (5 ϫ 10 Ϫ6 -0.5% [wt/vol]) in normal medium containing serum or without serum testing four wells per concentration.…”

Evaluation of a Gelatin-Modified Poly(ε-Caprolactone) Film as a Scaffold for Lung Disease

“…Compared to cells with limited lifetime, immortalized cell lines have indefinite possibility to be grown in culture which makes them suitable for in vitro analysis of biocompatibility of synthetic polymers (Altman, Randers, and Rao 1993;Zange, Li, and Kissel 1998;Huhtala et al 2007;Singh, Wu, and Dunn 2011).…”

“…The in vitro environment offers a setting to test the effects of the synthetic material on cell adhesion, proliferation, and viability (Huhtala et al 2007). From this study, any cytotoxic effects related directly to the presence of the polymer may be assessed (Altman, Randers, and Rao 1993).…”

“…From this study, any cytotoxic effects related directly to the presence of the polymer may be assessed (Altman, Randers, and Rao 1993). In vitro models offer a relatively inexpensive, rapid method to provide initial biocompatibility screening that reduces the number of animal studies to be carried out (Zange, Li, and Kissel 1998;Huhtala et al 2007). In this study, the cell line of choice was the human epithelial cell line NCI-H292.…”

Production and in vitro evaluation of macroporous alginate hydrogel fibres for nerve tissue engineering

“…In line with general scaffold design criteria, the ideal scaffold material for retinal repair must be biocompatible, biodegradable and mechanically stable while not inducing immunogenic responses [300]. Earlier studies [301][302][303] have recommended that the scaffold should be relatively thin, less than 150 μm (compare the thickness of the photoreceptor layer of 155.6 ± 12.6 μm in normal adults) [304] to avoid physical distortion of the overlying retina after transplantation.…”