In Vitro and in Vivo Mechanism of Bone Tumor Inhibition by Selenium-Doped Bone Mineral Nanoparticles

Abstract

Biocompatible tissue-borne crystalline nanoparticles releasing anticancer therapeutic inorganic elements are intriguing therapeutics holding the promise for both tissue repair and cancer therapy. However, how the therapeutic inorganic elements released from the lattice of such nanoparticles induce tumor inhibition remains unclear. Here we use selenium-doped hydroxyapatite nanoparticles (Se-HANs), which could potentially fill the bone defect generated from bone tumor removal while killing residual tumor cells, as an example to study the mechanism by which selenium released from the lattice of Se-HANs induces apoptosis of bone cancer cells in vitro and inhibits the growth of bone tumors in vivo. We found that Se-HANs induced apoptosis of tumor cells by an inherent caspase-dependent apoptosis pathway synergistically orchestrated with the generation of reactive oxygen species. Such mechanism was further validated by in vivo animal evaluation in which Se-HANs tremendously induced tumor apoptosis to inhibit tumor growth while reducing systemic toxicity. Our work proposes a feasible paradigm toward the design of tissue-repairing inorganic nanoparticles that bear therapeutic ions in the lattice and can release them in vivo for inhibiting tumor formation.

Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits

“…Briefly, PCL (Mw = 50,000) (Daigang Biomaterial Co., Ltd, China) was dissolved in dichloromethane. HA nanoparticles with the size distribution of 80–100 nm were synthesized using previously published protocol [32–34]), and dispersed sufficiently in ethyl alcohol by ultrasonication. The two solutions were mixed sufficiently and added dropwise into a solution of 1.5% (w/v) poly(vinyl alcohol).…”

Selenium-Doped Hydroxyapatite Nanocrystals–Synthesis, Physicochemical Properties and Biological Significance

“…HA doped with selenium ions can be obtained in two ways: through synthesis, during which ions are incorporated into the HA structure [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47], or by ion exchange, where soaking in a selenium salt solution results in the exchange of ions in the HA structure [47].…”

Effect of Selenium Nanoparticles on Anti-Oxidative Level, Egg Production and Quality and Blood Parameter of Laying Hens Exposed to Deoxynivalenol

“…The selenium nanoparticle (SeNP), which is one form of elemental selenium (Se0), has lower toxicity and better absorption and it is most effective when provided to broiler chicken under oxidative stress with the same dose but different sources of Se (organic, inorganic and nano-Se) [28]. According to some studies, the size of SeNPs synthesized by microorganisms has mostly been between 20 and 300 nm [20,26,27], and the diameter of the SeNPs in this research was 50 to 110 nm, which is consistent with previous research.…”

“…Wang et al [20] found Se-HANs induced apoptosis of tumor cells by an inherent caspase-dependent apoptosis pathway synergistically orchestrated with the generation of reactive oxygen species. In addition, another research reported that selenite-doped bone mineral nanoparticles can retard the growth of osteosarcoma in a nude mice model [21].…”