Fusarium species detected in onychomycosis in Colombia

Abstract

Fusarium spp. have frequently been isolated from patients with onychomycosis. In Colombia, several studies have shown that Fusarium is the most common non-dermatophyte mould causing onychomycosis and its spread has increased in the past years. In this study, samples were collected in 2003 and 2004 from 137 patients who were diagnosed with onychomycosis caused by Fusarium spp. Three species of Fusarium were identified: Fusarium solani (64.9%), Fusarium oxysporum (32.8%) and Fusarium verticillioides (2.3%). The diseases were more common in women (73%) than in men (27%) and occurred mainly among adults between 31 and 40 years old. The percentage of patients who had received previous treatments was 63.5%. In the last years, new and improved antifungal agents like echinocandins or new triazoles like voriconazole have been developed. For this reason, susceptibility testing using voriconazole was performed, by broth microdilution and disk diffusion. The results showed that F. solani had the highest minimum inhibitory concentration. Using the disk diffusion test, many of the isolates showed variable susceptibility. Genetic diversity of F. oxysporum isolates was determined by random amplified polymorphic DNA. Twenty isolates belonging to different haplotypes were selected for PCR amplification of a region of the gene encoding α-l-arabinofuranosidase B, a specific test to determine if the isolates were F. oxysporum f. sp. dianthi. On the basis of these PCR results, we found that five out of the 20 F. oxysporum isolates corresponded to f. sp. dianthi.

Emergence of fusarioses in a university hospital in Turkey during a 20-year period

“…Also, in our study, F. proliferatum was the predominant species in locally invasive infections and along with F. petroliphilum in disseminated infections [33]. The FSSC has also been reported as the most commonly isolated species complex in superficial infections, mainly keratitis and onychomycosis, in America and Asia, while the FOSC ranks first in such infections in Europe [9,[34][35][36]. Although the number of cases was low in our study, the FSSC was responsible in 85.7 % of superficial infections.…”

In vitro Antifungal Susceptibility Testing of Clinical and Environmental Fusarium Isolates in Iran

“…Although, the distribution of Fusarium species varies globally, Fusarium solani, F. oxysporum and F. verticillioides are the most frequent causes of fusariosis (16,17). Molecular phylogenetic studies have revealed that this genus comprises species complexes and the majority of Fusarium isolates cannot be identified to the species level using traditional morphological methods (4).…”

Oral Terbinafine and Itraconazole Treatments against Dermatophytes Appear Not to Favor the Establishment of <b><i>Fusarium</i></b> spp. in Nail

“…Fusarium spp. was also frequently isolated from onychomycosis in Brazil and Colombia, Fusarium oxysporum and Fusarium solani being the most prevalent species [9,10]. …”