Escherichia coliIsolates from Broiler Chicken Meat, Broiler Chickens, Pork, and Pigs Share Phylogroups and Antimicrobial Resistance with Community-Dwelling Humans and Patients with Urinary Tract Infection

Abstract

Escherichia coli is the most common cause of urinary tract infection (UTI). Phylogroup B2 and D isolates are associated with UTI. It has been proposed that E. coli causing UTI could have an animal origin. The objective of this study was to investigate the phylogroups and antimicrobial resistance, and their possible associations in E. coli isolates from patients with UTI, community-dwelling humans, broiler chicken meat, broiler chickens, pork, and pigs in Denmark. A total of 964 geographically and temporally matched E. coli isolates from UTI patients (n = 102), community-dwelling humans (n = 109), Danish (n = 197) and imported broiler chicken meat (n = 86), Danish broiler chickens (n = 138), Danish (n = 177) and imported pork (n = 10), and Danish pigs (n = 145) were tested for phylogroups (A, B1, B2, D, and nontypeable [NT] isolates) and antimicrobial susceptibility. Phylogroup A, B1, B2, D, and NT isolates were detected among all groups of isolates except for imported pork isolates. Antimicrobial resistance to three (for B2 isolates) or five antimicrobial agents (for A, B1, D, and NT isolates) was shared among isolates regardless of origin. Using cluster analysis to investigate antimicrobial resistance data, we found that UTI isolates always grouped with isolates from meat and/or animals. We detected B2 and D isolates, that are associated to UTI, among isolates from broiler chicken meat, broiler chickens, pork, and pigs. Although B2 isolates were found in low prevalences in animals and meat, these sources could still pose a risk for acquiring uropathogenic E. coli. Further, E. coli from animals and meat were very similar to UTI isolates with respect to their antimicrobial resistance phenotype. Thus, our study provides support for the hypothesis that a food animal and meat reservoir might exist for UTI-causing E. coli.

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

“…Similar to other studies worldwide, the majority of the FEC isolates were phylogenetic group C, A and B1 (Hiki et al, 2014;Jakobsen et al, 2010;Sola-Gines et al, 2015). In this current study, fifty percent of the CEC isolates belonged to phylogenetic group A, which is typically identified as a commensal, non-pathogenic E. coli (Clermont et al, 2013).…”

“…by Jakobsen et al (2010), found E. coli isolates sourced from human patients, broiler meat and other food-producing animals, including pigs, could not be differentiated on the basis of their VGs nor their AMR profile. They found resistant isolates clustered in the same phylogenetic group regardless of the source of the isolates (Jakobsen et al, 2010).…”

“…They found resistant isolates clustered in the same phylogenetic group regardless of the source of the isolates (Jakobsen et al, 2010). Johnson et al (2012) reported an association between MDR and plasmid content among commensal E. coli (AFEC) and ExPEC obtained from humans and poultry.…”

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Raw Milk Samples in Al Jazirah State, Sudan

“…In recent years, ESBLproducing Enterobacteriaceae isolates have shifted from the hospital to the community and the environment [41]. ESBL-producing Enterobacteriaceae have been recovered from different sources in the community, including cattle, chickens, pigs, raw milk and lettuce [42][43][44]. Recent study from India reported that a substantial number of tap water samples were contaminated with carbapenemase blaNDM-1 producing organisms [45].…”

Scientific Opinion on the public health hazards to be covered by inspection of meat (swine)

“…In addition, E. coli carrying extended-spectrum beta-lactamases (ESBL) and AmpC ß-lactamases (AmpC) are increasingly being considered as an emerging public health concern. Antimicrobial resistant E. coli (Gousia et al, 2011;Jakobsen et al, 2010) and VRE (Freitas et al, 2011;Gambarotto et al, 2001;Kempf et al, 2008;Manero et al, 2006) have been isolated from pigs and pork. ESBL-and AmpCproducing bacterial strains and genes relevant for public health have also been reported in pigs and pork products (in particular CTX-M, TEM-52, SHV-12 and CMY-2) (EFSA, 2011b).…”