I. Erythrocyte membrane stabilization by tranquilizers and antihistamines

Seeman, Philip; Weinstein, Jeffrey L.

doi:10.1016/0006-2952(66)90081-5

Cited by 231 publications

(66 citation statements)

References 26 publications

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“…The suppressive effect of lidocaine on the filopodial sprouting is well interpreted by assuming that lidocaine stabilizes the structure of phospholipid membrane in addition to blocking ionic channels. In fact, surface active agents are also known to stabilize membranes against hypotonic and mechanical stresses (Seeman and Weinstein, 1966). Further analyses are necessary to determine which of the above two possibilities is involved in the rapid filopodial sprouting.…”

Section: Dependence On Ca2+mentioning

confidence: 99%

Rapid sprouting of filopodia in nerve terminals of chromaffin cells, PC12 cells, and dorsal root neurons induced by electrical stimulation

Manivannan

Terakawa

1994

J. Neurosci.

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Rapid morphological changes induced by direct electrical stimulation of nerve terminals were studied by using video-enhanced differential interference contrast microscopy at a very high magnification (12,000x). We used mainly cultured bovine chromaffin cells, which developed neurite-like processes, and PC12 cells, which showed neuronal differentiation upon NGF treatment. In a few cases, primary neurons of the rat dorsal root ganglion were also examined. Brief pulse stimulation of the terminals and varicosities induced exocytosis accompanied by rapid formation of filopodia. These filopodia, 0.1–0.2 micron in diameter and up to 10 microns in length, formed within a few hundreds of milliseconds and then retracted within tens of seconds. They could also be induced by K depolarization. This rapid filopodial sprouting strongly depended on the presence of extracellular Ca2+ and could be abolished in a medium containing a Ca chelator (EGTA) or La2+. Anti-cytoskeletal agents colchicine and cytochalasin B failed to block this response completely but lidocaine fully suppressed it. Quantitative analysis of exocytosis and filopodial sprouting showed that they were independent events, not directly linked to each other, having different thresholds usually higher for filopodial formation. In PC12 cells, the extent of filopodial sprouting varied with the state of differentiation of the cells, suggesting a functional role of rapid sprouting during a particular phase of their differentiation. Filopodia could be induced with greater ease by repetitive stimulation. The same responses may occur at growth cones approaching the target cells or even at mature synapses particularly after repetitive electrical activity, possibly playing a role in use-dependent synapse formation or plasticity.

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Section: Dependence On Ca2+mentioning

confidence: 99%

Rapid sprouting of filopodia in nerve terminals of chromaffin cells, PC12 cells, and dorsal root neurons induced by electrical stimulation

Manivannan

Terakawa

1994

J. Neurosci.

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“…A variety of drugs, most of them lipid-soluble, can protect erythrocytes against osmotic lysis. Amongst known protective agents are amyl alcohol (Traube, 1908), several volatile anaesthetics (Jacobs & Parpart, 1932), tranquillizers and antihistaminics (Seeman & Weinstein, 1966), steroids and antiinflammatory drugs (Seeman, 1966a, Inglot & Wolna, 1968Brown, Taylor & Waters, 1971) fat-soluble vitamins (Seeman, 1966b) and propranolol (Fortier, Snyder. Palek & Weiss, 1977).…”

Section: Discussionmentioning

confidence: 99%

EFFECTS OF SOME S‐ALKYLTHIOURONIUMS AND RELATED COMPOUNDS ON THE OSMOTIC FRAGILITY AND THE MEMBRANE EXPANSION OF HUMAN ERYTHROCYTES

Beresford

Fastier

1980

British J Pharmacology

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1 Changes in the osmotic fragility and critical haemolytic volume of human erythrocytes produced by S-n-decylthiouronium (S-10) and related compounds have been studied. 2 S-10 had a biphasic action on osmotic fragility, protecting erythrocytes against lysis in low concentrations but producing lysis in a concentration of 1 mm or higher. 3 Some lower homologues of S-10 also protected erythrocytes against osmotic lysis, the degree of protection depending on the length of the alkyl chain. 4 Critical haemolytic volume was increased by anthihaemolytic concentrations of procaine and chlorpromazine but not by antihaemolytic concentrations of S-10 and related amidines. 5 It is concluded that S-10 and its near homologues penetrate and stabilize erythrocyte membranes, potency increasing with the number of methylene groups in the side-chain up to about ten. The stabilization produced by S-10 apparently differs from that produced by many other lipid-soluble depressant drugs. It may be related to a drug-induced change in the ionic permeability of the membrane.

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“…In general, the binding of anesthetics to the membrane is reversible (Seeman and Weinstein 1966) . At high concentrations of anesthetics, however, irreversible exposure of new binding sites in the membrane and sharp rise of the membrane/ buffer partition coefficient were reported (Colley et al .…”

Section: Discussionmentioning

confidence: 99%