Decrease and recovery in cell surface charge induced by anesthetics.

Satõ, Kazuya; Kojima, Kiyohide; Sato, Chikara

doi:10.1620/tjem.123.185

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…The details of the construction were described in ref. [3]. The constructed plasmid was transformed in to S. cerevisiae strain BY4741 by applying the kit S. cerevisiae Direct Transformation Kit Wako (Wako Pure Chemical, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Construction of a Cell Surface Engineered Yeast Aims to Selectively Recover Molybdenum, a Rare Metal

Chien¹,

Ikeda

Kubota

et al. 2017

SSP

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The depletion of rare metals is an issue of major concern since rare metals are limited in the abundance but essential for high technology industry. However, the present rare metal recovery technical by chemical methods has high environmental impact, poor selectivity, and is too expensive to be practical. To resolve these problems, this study aimed to create a rare metal recover system using yeast, and molybdenum was selected as the first target. A molybdenum binding protein, ModE, which was derived from Escherichia coli was selected. A fusion gene was generated by linking partial modE with a secretion signal and a domain of α-agglutinin to display the ModE on the surface of yeast cells. The expression of fusion protein on the cell surface was detected by immunofluorescence labeling. As for the recovery experiment, the engineered yeast cells were incubated in 10 mM of sodium molybdate solution for 2 h, and the recovery of molybdenum ion was measured by ICP-AES. The results of fluorescence micrographs showed that the designed fusion protein was successfully expressed on yeast cell surface. According to the results of ICP-AES, the cell surface engineered yeast adsorbed molybdenum and the cells after 72~84 h incubation gave the best adsorption. Besides, the results suggested that the optimization of each functional domain in the fusion protein was important. The selectivity and the lower limit of recoverable concentration are under investigation, while this study provides a preliminary result of bio-extraction technology using cell surface engineered yeast.

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Section: Methodsmentioning

confidence: 99%

Construction of a Cell Surface Engineered Yeast Aims to Selectively Recover Molybdenum, a Rare Metal

Chien¹,

Ikeda

Kubota

et al. 2017

SSP

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A Mechanism for Tamoxifen-mediated Inhibition of Acidification

Chen

Schindler

Simon

1999

Journal of Biological Chemistry

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Tamoxifen has been reported to inhibit acidification of cytoplasmic organelles in mammalian cells. Here, the mechanism of this inhibition is investigated using in vitro assays on isolated organelles and liposomes. Tamoxifen inhibited ATP-dependent acidification in organelles from a variety of sources, including isolated microsomes from mammalian cells, vacuoles from Saccharomyces cerevisiae, and inverted membrane vesicles from Escherichia coli. Tamoxifen increased the ATPase activity of the vacuolar proton ATPase but decreased the membrane potential (V m ) generated by this proton pump, suggesting that tamoxifen may act by increasing proton permeability. In liposomes, tamoxifen increased the rate of pH dissipation. Studies comparing the effect of tamoxifen on pH gradients using different salt conditions and with other known ionophores suggest that tamoxifen affects transmembrane pH through two independent mechanisms. First, as a lipophilic weak base, it partitions into acidic vesicles, resulting in rapid neutralization. Second, it mediates coupled, electroneutral transport of proton or hydroxide with chloride. An understanding of the biochemical mechanism(s) for the effects of tamoxifen that are independent of the estrogen receptor could contribute to predicting side effects of tamoxifen and in designing screens to select for estrogen-receptor antagonists without these side effects.Tamoxifen is the most commonly used treatment for breast cancer (1). In addition, it is currently being considered for widespread use in healthy women for breast cancer prevention (2, 3). Yet, despite its widespread use, its mechanisms of action remain obscure. Tamoxifen is a known estrogen receptor modulator that acts as an antagonist or partial agonist. But it has also been reported to have many pleiotropic effects both in vivo and in vitro that cannot be explained by an interaction with the estrogen receptor (4). For example, tamoxifen has been shown to enhance drug sensitivity of multidrug-resistant cells (5-9), inhibit bone resorption and osteoporosis both in vivo and in vitro (10), and inhibit a number of channels, including the volume activated chloride channel (11, 12) and calcium channels (13-16). These effects have been attributed to inhibition of P-glycoprotein (17), calmodulin (15), and direct channel interaction (11), respectively.Previously, we have observed that tamoxifen inhibits acidification of intracellular organelles of both estrogen receptor positive and negative cell lines (18). This inhibition of acidification may be a mechanism for many of the effects of tamoxifen. For example, the effects of tamoxifen on osteoporosis (19), vesicular transport (20, 21), or multidrug resistance (9, 22) are mimicked by blocking the proton vATPase 1 or by a protonophore. This work addresses the mechanism(s) by which tamoxifen inhibits ATP-dependent in vitro acidification of organelles isolated from tissue culture cells, whole tissue, vacuoles from Saccharomyces cerevisiae, and inverted vesicles isolated from Escherichia coli. The ...

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Dislocation of Sialic Acid on Erythrocytes Membrane by nesthetics and its Blocking by SH-reagents, Colchicine r Cytochalasin B

Sato

Kojima

Nishizawa

et al. 1978

Cell Struct. Funct.

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ABSTRACT. The cell electrophoretic mobility of rat erythrocytes decreased by treatment with thiopental or droperidol (intravenous anesthetics) at clinical concentrations.The amount of sialic acid, the main determinant of electrophoretic mobility, was not changed by the anesthetics. By using phosphate buffers of different ionic strengths, which determines the effective thickness of the ionic layer for electrophoretic mobility, translocation of sialic acid from the most peripheral zone to a deeper zone was suggested. When mobility recovered by washing out of the drugs followed by subsequent incubation, sialic acid was restored to the peripheral zone. The decrease in mobility was inhibited by sulfhydryl-blocking agents and disulfide-bridging agent. Cytochalasin B and colchicine completely blocked the mobility reduction when used single, but not when used simultaneously. These results suggest conformational changes of membrane sialoprotein by anesthetics involving sulfhydryls and cytochalasin B-and colchicine-sensitive proteins.

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Decrease and recovery in cell surface charge induced by anesthetics.

Cited by 3 publications

References 13 publications

Construction of a Cell Surface Engineered Yeast Aims to Selectively Recover Molybdenum, a Rare Metal

Construction of a Cell Surface Engineered Yeast Aims to Selectively Recover Molybdenum, a Rare Metal

A Mechanism for Tamoxifen-mediated Inhibition of Acidification

Dislocation of Sialic Acid on Erythrocytes Membrane by nesthetics and its Blocking by SH-reagents, Colchicine r Cytochalasin B

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