Corynebacterium pyruviciproducens, as an immune modulator, can promote the activity of macrophages and up-regulate antibody response to particulate antigen

Abstract

Corynebacterium pyruviciproducens is a newly discovered Corynebacterium species with no known pathogenic components such as diphtheria toxin and tuberculostearic acid, and it has similar biological properties to Propionibacterium acnes, but its role of immunoregulation is drawing people's attention. In this work, based on the role of macrophages in removal of pathogenic bacteria as a primary scavenger and particulate antigen-presenting cell, the stimulation of macrophages by C. pyruviciproducens was analyzed through detecting the levels of cytokine secretion and expression of membrane molecules, and the effect of C. pyruviciproducens in promoting antibody response to sheep red blood cells (SRBC) in vivo was detected. In vitro, C. pyruviciproducens led to a sharp release of interleukin-6 and tumour necrosis factor-α and encouraged the activation of macrophages including enhanced expressions of MHC-II, CD40, CD80 and CD86. In vivo, it enhanced the humoral immune response against SRBC, a particulate antigen. These observations suggest that C. pyruviciproducens, as an immunoregulator, can promote the host humoral immune response to pathogenic microorganisms by regulating macrophage function.

Effects of lysine biomass supplementation on growth performance and clinical indicators in broiler chickens

“…Other than stress, such immune responses could also be attributed to the LB product containing killed cells of the bacteria C. glutamicum . Tong et al. (2012) found that killed bacteria cells influence the immune system in rodents and Ebisawa et al.…”

CP and CP-PGN protect mice against MRSA infection by inducing M1 macrophages

“…C. pyruviciproducens is a newly discovered bacterial immunoregulation without known pathogenic components. C. pyruviciproducens showed a stronger immunomodulatory effect than P. acnes of a well-known traditional immune adjuvant, confirmed by experiments with different types of pattern antigens, such as ovalbumin and sheep red blood cell 1 – 3 . This report is to investigate whether C. pyruviciproducens has a strong ability to resist infection caused by clinical pathogenic microorganisms, like Staphylococcus aureus ( S. aureus ).…”

“…The RAW264.7 cell line (kept by our laboratory) was cultured in RPMI1640 medium containing 10% heat-inactivated FBS at 37 °C, 5% CO 2 atmosphere. Peritoneal macrophages were derived from BALB/c mice, referring to the previous reports 3 . All the experiments were divided into three groups: control group; C. pyruviciproducens group (CP group); peptideglyean of CP group (CP-PGN group).…”

Insight into the intestinal microbiome of farrowing sows following the administration of garlic (Allium sativum) extract and probiotic bacteria cultures under farming conditions

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