Chlorella vulgaris ameliorates testicular toxicity induced by deltamethrin in male rats via modulating oxidative stress

Abstract

This study was carried out to investigate the potential effects of Chlorella vulgaris (CV) on testicular function and oxidant/antioxidant status in normal and deltamethrin‐intoxicated rats. Forty adult male rats were drenched either with normal saline, CV (50 mg/kg), deltamethrin (DM) (3 mg/kg), or CV combined with DM, daily for 8 weeks. At the end of the protocol, the epididymal sperm quality was evaluated and the testicular superoxide dismutase (SOD), catalase enzyme (CAT) and malondialdehyde (MDA), and the serum testosterone levels were estimated. Normal rats treated with CV showed a significant increase in the total sperm number/epididymal tail, testicular SOD, and CAT levels with a significant decrease in the testicular MDA. Deltamethrin intoxication significantly decreased the proportions of motile and live sperm, the testosterone concentration, the testicular SOD and CAT levels, whereas it significantly increased the proportion of abnormal sperm and the testicular MDA. Chlorella vulgaris treatment significantly ameliorated the adverse effects of DM‐intoxication and restored most of the parameters to levels that are comparable to those of the control group. In conclusion, CV administration improved the testicular function of normal rats and ameliorated the effect of severe oxidative stress conditions.

Methanolic extract of Chlorella vulgaris protects against sodium nitrite‐induced reproductive toxicity in male rats

“…The antioxidant property of CV might counteract and prevent the negative, disruptive effect of oxidative stress on spermatogenesis, male reproductive hormonal profiles, and hypothalamic–pituitary–gonadal axis. These results are consistent with those of the previous studies (Bansal & Bilaspuri, 2010; Osama et al., 2018). Supplementation with a source of polyunsaturated fatty acids (PUFAs) like commercial algal product found to improve the sperm count and motility (Khoshvaght et al, 2016; Murphy et al, 2017).…”

“…The healing effect of CV could be attributed to its powerful antioxidant activities with subsequently associated reduced inflammatory condition; the current findings were supported by data of Osama et al. (2018).…”

“…Percentages of motile sperm cells subjectively were evaluated using high‐power magnification (40×) with the help of a phase‐contrast microscope provided by the hot stage (Mousa et al., 2019; Osama et al., 2018; Saber, Abd El‐Aziz, & Ali, 2016). About 10 µl of sperm suspension was allocated in Neubauer chamber and used to evaluate the total sperm count as described by Yari et al (2016).…”

“…Additionally, it was shown that Chlorophylls of Chlorella vulgaris prevent lipid peroxidation via reducing ROS and chelating metal ions (Hsu, Chao, Hu, & Yang, 2013). Treatment with CV restored the spermatogenic activity, androgenic activity, sperm viability, sperm cell count, and the testicular oxidant/antioxidant status in rat intoxicated with deltamethrin, and this effect was attributed to its antioxidant activity (Osama, Galal, Abdalla, & El‐Sheikh, 2018). The present study was designed to evaluate the potential protective effects of CV against the reproductive toxicity effect of sodium nitrite.…”

Effect of Nigella sativa and Foeniculum vulgare seeds extracts on male mice exposed to carbendazim

“…Zari and Al-Attar (2011) showed that treatment of male rats with CBZ resulted in severe damage and completely absences of spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa and losses of the spermatogenesis process. Moreover, adverse reproductive effects attributed to pesticides, including their effect on fertility, have also been well established in various experimental studies ( Hassan and Meligi, 2017 , El-Demerdash et al, 2019 , Ghorbani Taherdehi et al, 2019 , Osama et al, 2019 )…”