Chlamydia trachomatis detection in cervical PreservCyt specimens from an Irish urban female population

Abstract

Objective: The aim of this study was to determine the prevalence of cervical Chlamydia trachomatis infection by polymerase chain reaction (PCR) in urban women undergoing routine cervical cytological screening and to investigate the relationship with age, cytology, smoking status and concurrent human papillomavirus (HPV) infection. Methods: A total of 996 women (age range 16–69 years) attending general practitioners for routine liquid‐based cervical smear screening in the Dublin area were recruited in the study of prevalence of C. trachomatis. Informed consent was obtained and liquid‐based cytology (LBC) specimens were sent for cytological screening. DNA was extracted from residual LBC and tested for C. trachomatis by PCR using the highly sensitive C. trachomatis plasmid (CTP) primers and for HPV infection using the MY09/11 primers directed to the HPV L1 gene in a multiplex format. Results: The overall prevalence of C. trachomatis was 5.4%. Prevalence was highest in the <25 years age group (10%). Coinfection with HPV and C. trachomatis occurred in 1% of the screening population. A higher rate of smoking was observed in women positive for C. trachomatis, HPV infections or those with abnormal cervical cytology. Chlamydia trachomatis infection was not associated with abnormal cytology. Conclusions: Women (5.4%) presenting for routine cervical screening are infected with C. trachomatis. Opportunistic screening for C. trachomatis from PreservCyt sample taken at the time of cervical cytological screening may be a possible strategy to screen for C. trachomatis in the Irish female population.

Helicobacter pylori resistance to metronidazole and clarithromycin in Ireland

“…The rate of CLA resistance was also slightly higher in the female population, although none have reached the statistical significance. Macrolide antibiotics may be used for the treatment of Chlamydia infection that is prevalent in 5% of females in our catchment area [38].…”

Chlamydia trachomatis among women with normal and abnormal cervical smears in Lagos, Nigeria

“…29,32 This is probably due to the decreased frequency of sexual activity with age thus leading to a reduction in the prevalence in the older age group.…”

Chlamydia trachomatis Detection by Nested-PCR Method on Females Referred to Medical Centers of Tehran, Iran

“…Chlamydia trachomatis, Ureaplasma urealyticum, Gardnerella vaginalis, Listeria monocytogenes and Neisseria gonorrhoeae are some of the bacteria localize in female genital tract (1). Chlamydia trachomatis (C. trachomatis) is the most causative agent of bacterial sexually transmitted infections (STI) (2,3). There are about 90 million cases with STI throughout the world, annually (3).…”

“…Chlamydia trachomatis (C. trachomatis) is the most causative agent of bacterial sexually transmitted infections (STI) (2,3). There are about 90 million cases with STI throughout the world, annually (3). It can cause cervicitis, pelvic inflammatory disease (PID), and urethritis in women (4).…”