<i>Caenorhabditis elegans</i> in Chinese Medicinal Studies: Making the Case for Aging and Neurodegeneration

Wang, Qiangqiang; Yang, Fan; Guo, Wei; Zhang, Ju; Xiao, Lingyun; Li, Haifeng; Jia, Weizhang; Huang, Zebo

doi:10.1089/rej.2013.1512

Cited by 28 publications

(23 citation statements)

References 17 publications

(18 reference statements)

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“…As lifespan is regarded as an unequivocal anti-aging index [ 22 ], we further investigated the effect of AsiPeps on the lifespan of C. elegans and showed that the lifespan of the nematodes was indeed extended after treatment with 2.0 and 4.0 mg/mL of the peptides ( Figure 5(b) ). Since the nematodes may cut down their consumption of food if a test sample is distasteful, dietary restriction, which is well known to retard development and delay aging in C. elegans [ 41 ], may contribute to the lifespan-extending effect of AsiPeps.…”

Section: Resultsmentioning

confidence: 99%

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Bioactive Peptides from Angelica sinensis Protein Hydrolyzate Delay Senescence in Caenorhabditis elegans through Antioxidant Activities

Wang

Yunxuan

Qin

et al. 2016

Oxidative Medicine and Cellular Longevity

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Since excessive reactive oxygen species (ROS) is known to be associated with aging and age-related diseases, strategies modulating ROS level and antioxidant defense systems may contribute to the delay of senescence. Here we show that the protein hydrolyzate from Angelica sinensis was capable of increasing oxidative survival of the model animal Caenorhabditis elegans intoxicated by paraquat. The hydrolyzate was then fractionated by ultrafiltration, and the antioxidant fraction (<3 kDa) was purified by gel filtration to obtain the antioxidant A. sinensis peptides (AsiPeps), which were mostly composed of peptides with <20 amino acid residues. Further studies demonstrate that AsiPeps were able to reduce the endogenous ROS level, increase the activities of the antioxidant enzymes superoxide dismutase and catalase, and decrease the content of the lipid peroxidation product malondialdehyde in nematodes treated with paraquat or undergoing senescence. AsiPeps were also shown to reduce age pigments accumulation and extend lifespan but did not affect the food-intake behavior of the nematodes. Taken together, our results demonstrate that A. sinensis peptides (AsiPeps) are able to delay aging process in C. elegans through antioxidant activities independent of dietary restriction.

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Section: Resultsmentioning

confidence: 99%

“…The lifespan assay was performed in 96-well plates using liquid culture as previously described [ 21 ]. The number of live nematodes was scored microscopically every 2 days based on their mobility, shape, and pharyngeal pumping until all the nematodes died [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Bioactive Peptides from Angelica sinensis Protein Hydrolyzate Delay Senescence in Caenorhabditis elegans through Antioxidant Activities

Wang

Yunxuan

Qin

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…Although C. elegans is a tiny organism (~1 mm in length), it contains about two-thirds of the potential counterparts of human disease genes and nearly one-third of its somatic cells are neurons [13]. Along with its short lifespan, genetic tractability and multiple behavioral phenotypes, this nematode model also offers special advantages in studies of age-onset neurodegenerative disorders [14]. For example, the pan-neuronal expression of human Aβ42 protein in C. elegans strain CL2355 causes behavioral deficits in chemotaxis, associative learning and thrashing [15].…”

Section: Introductionmentioning

confidence: 99%

Salidroside Protects Caenorhabditis elegans Neurons from Polyglutamine-Mediated Toxicity by Reducing Oxidative Stress

Xiao

Zhang

et al. 2014

Molecules

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Polyglutamine (polyQ) aggregation plays a pivotal role in the pathological process of Huntington's disease and other polyQ disorders. Therefore, strategies aiming at restoring dysfunction and reducing stresses mediated by polyQ toxicity are of therapeutic interest for proteotoxicity diseases. Salidroside, a glycoside from Rhodiola rosea, has been shown to have a variety of bioactivities, including antioxidant activity. Using transgenic Caenorhabditis elegans models, we show here that salidroside is able to reduce neuronal death and behavioral dysfunction mediated by polyQ expressed in ASH neurons, but the neuroprotective effect is not associated with prevention of polyQ aggregation per se. Further experiments reveal that the neuroprotective effect of salidroside in C. elegans models involves its antioxidant capabilities, including decrease of ROS levels and paraquat-induced mortality, increase of antioxidant enzyme activities and reduction of

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“…Nevertheless, sequence information and regulation mechanisms of bioactive peptides in M. meretrix are lacking. Here we first examined the in vivo antioxidant properties of a peptide-rich extract of M. meretrix using Caenorhabditis elegans , a powerful model animal widely used in biomedical studies [ 26 ]. After separation of the antioxidant peptide fractions, we then identified the peptide sequences and synthesized the peptides for bioactivity studies.…”

Section: Introductionmentioning

confidence: 99%

Novel Bioactive Peptides from Meretrix meretrix Protect Caenorhabditis elegans against Free Radical-Induced Oxidative Stress through the Stress Response Factor DAF-16/FOXO

Jia

Peng

et al. 2018

Marine Drugs

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The hard clam Meretrix meretrix, which has been traditionally used as medicine and seafood, was used in this study to isolate antioxidant peptides. First, a peptide-rich extract was tested for its protective effect against paraquat-induced oxidative stress using the nematode model Caenorhabditis elegans. Then, three novel antioxidant peptides; MmP4 (LSDRLEETGGASS), MmP11 (KEGCREPETEKGHR) and MmP19 (IVTNWDDMEK), were identified and were found to increase the resistance of nematodes against paraquat. Circular dichroism spectroscopy revealed that MmP4 was predominantly in beta-sheet conformation, while MmP11 and MmP19 were primarily in random coil conformation. Using transgenic nematode models, the peptides were shown to promote nuclear translocation of the DAF-16/FOXO transcription factor, a pivotal regulator of stress response and lifespan, and induce the expression of superoxide dismutase 3 (SOD-3), an antioxidant enzyme. Analysis of DAF-16 target genes by real-time PCR reveals that sod-3 was up-regulated by MmP4, MmP11 and MmP19 while ctl-1 and ctl-2 were also up-regulated by MmP4. Further examination of daf-16 using RNA interference suggests that the peptide-increased resistance of C. elegans to oxidative stress was DAF-16 dependent. Taken together, these data demonstrate the antioxidant activity of M. meretrix peptides, which are associated with activation of the stress response factor DAF-16 and regulation of the antioxidant enzyme genes.

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Caenorhabditis elegans in Chinese Medicinal Studies: Making the Case for Aging and Neurodegeneration

Cited by 28 publications

References 17 publications

Bioactive Peptides from Angelica sinensis Protein Hydrolyzate Delay Senescence in Caenorhabditis elegans through Antioxidant Activities

Bioactive Peptides from Angelica sinensis Protein Hydrolyzate Delay Senescence in Caenorhabditis elegans through Antioxidant Activities

Salidroside Protects Caenorhabditis elegans Neurons from Polyglutamine-Mediated Toxicity by Reducing Oxidative Stress

Novel Bioactive Peptides from Meretrix meretrix Protect Caenorhabditis elegans against Free Radical-Induced Oxidative Stress through the Stress Response Factor DAF-16/FOXO

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