Brenneria quercina and Serratia spp. isolated from Spanish oak trees: molecular characterization and development of PCR primers

Abstract

Brenneria quercina has been reported as one of the causal agents of oak decline in Spain. To investigate the bacterial variability of this pathogen from different Spanish oak forests, a collection of 38 bacterial isolates from seven geographic locations and from different oak species was analysed by sequencing 16S rDNA and rep-PCR fingerprinting. All Spanish isolates of B. quercina were grouped by rep-PCR into a homogenous cluster that differed significantly from B. quercina reference strains from California. 16S rDNA analysis revealed that 34 out of 38 isolates were Brenneria . However, four isolates belonged to the genus Serratia , suggesting that this bacterium could cause cankers in oak trees. The information obtained by rep-PCR fingerprint analysis was used to develop PCR primers for the sensitive and specific detection of B. quercina from infected plant tissues. Pathogenicity tests performed with Brenneria and Serratia isolates showed that both were able to grow and cause cankers in oak trees.

Genetic variability of plant-associated proteobacteria isolated from Slovak (Central European) territory

“…Our analysis indicated that the Bq129/08 strain belongs to Lonsdalea quercina subsp. britannica taxon, which is known as an oak tree pathogen previously isolated in several different countries (Poza-Carrión et al 2008;Brady et al 2011). Because the symptoms of disease caused by L. quercina subsp.…”

A new method to detect Lonsdalea quercina in infected plant tissues by real‐time PCR

“…Comparing with the rep‐PCR (repetitive element PCR fingerprinting technique) that was developed by Poza‐Carrión et al. () in the detection of L. quercina , real‐time PCR assay can rapidly identify and quantify the pathogen from genomic DNA, bacterial cells or directly from the tissue of field samples. This is because it has a number of specific advantages in the detection.…”

Revisión de técnicas de diagnóstico de Brenneria spp en nogal (Juglans regia)

“…Among the molecular diagnose methods is the Polymerase Chain Reaction (PCR) (Poza-Carrión et al, 2008;Frutos D, 2010). McClean et al (2008) developed real time PCR techniques based on the identification of B. rubrifaciens in inoculated soil and leaves, although they did not identify pure bacteria or infected samples.…”

“…Dentro de los métodos moleculares de diagnós-tico se encuentra la Reacción en Cadena de la Polimerasa (PCR) (Poza-Carrión et al, 2008;Frutos D, 2010). McClean et al, (2008) desarrollaron técni-rubrifaciencies using the gene responsible for the synthesis of rubrifaciens, the red pigment produced by B. rubrifaciens, present in this species only, as the author indicates.…”