Genetic variability of plant-associated proteobacteria isolated from Slovak (Central European) territory

Abstract

a department of general and Quarantine diagnostics, central control and testing institute of Agriculture, haniska, slovakia; b institute of Animal Physiology, slovak Academy of sciences, Košice, slovakia; c faculty of natural sciences, department of chemistry, ABSTRACT Genetic variability of 17 wild strains of γ-proteobacteria isolated from different host plants, locations and seasons, identified via routine diagnostics as Erwinia amylovora, was analysed. The ERIC-PCR confirmed the genetic homogeneity among 15 virulent strains while 2 avirulent strains were genetically distinct. These two avirulent strains differed in their antibiotic susceptibility from all virulent strains. The only avirulent Ra1051/98 strain showed significant ampicillin resistance. All 15 virulent strains were confirmed via the ERIC-PCR and MALDI-TOF MS, as closely related to the reference strain NCPPB 683 of E. amylovora. Avirulent strains were identified as Rahnella aquatilis and L. quercina subsp. britannica (formerly Brenneria quercina), by MALDI-TOF MS and the identification was confirmed by 16S rRNA gene sequence analysis. For the first time, L. quercina subsp britannica was identified in the fire blight-symptomatic samples in Central Europe, and for the first time this bacterium was isolated from the host different than oak.

Simulated microgravity shapes the endophytic bacterial community by affecting wheat root metabolism

“…2). Previous work has shown that Firmicutes and Proteobacteria can promote the growth of plants (Malik et al ., 2016; Qamar et al ., 2017). Our results suggest that the decrease in the relative abundance of Firmicutes and the increase in the relative abundance of Proteobacteria may be the reason for the poor growth and resistance of plants under space microgravity environments.…”