<i>Bifidobacterium longum</i> relieves constipation by regulating the intestinal barrier of mice

Wang, Linlin; Chai, Mao; Wang, Jialiang; Yu, Qiangqing; Wang, Gang; Zhang, Hao; Zhao, Jianxin; Chen, Wei

doi:10.1039/d1fo04151g

Cited by 25 publications

(25 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The determination of the content of fecal water referred to the method of Wang et al. (2022) and was calculated by the following equation:…”

Section: Methodsmentioning

confidence: 99%

“…Before the end, mice were given activated carbon solution, and the time from the end of gavage to the detection of the first black stool was recorded ( Wang et al., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Regulatory peptides of the gut levels (endothelin-1, ET-1; substance P, SP; gastrin, GAS; somatostatin, SS; motilin, MT; vasoactive intestinal peptide, VIP) in serum were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Wenle Biotechnology Co., Ltd., Shanghai, China) following the instructions. The contents of aquaporin 3 (AQP 3), occludin, interleukin-1β (IL-1β), and tumor necrosis factor (TNF-α) were measured by using the method described in a previous work ( Wang et al., 2022 ). Colon samples were treated with saline and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the meta-genomic DNA from mouse feces were extracted using the FastDNA Spin Kit for Feces (MP Biomedicals, Irvine, CA, USA). The V3–V4 regions were amplified using the 341F/806R primers ( Wang et al., 2022 ). After purification and quantification of PCR products, the production was sequenced using the Illumina MiSeq platform.…”

Section: Methodsmentioning

confidence: 99%

“…Functional constipation is a common gastrointestinal disease with a global incidence of more than 10% ( Johanson and Kralstein, 2007 ). The application of probiotics to relieve constipation is currently a research hotspot in the treatment of constipation ( Horiuchi et al., 2020 ; Lu et al., 2021 ; Park et al., 2021 ; Saviano et al., 2021 ; Wang et al., 2022 ). At present, the most effective probiotics for constipation are mainly Lactobacillu s and Bifidobacterium ( Matsumoto et al., 2006 ; Ishizuka et al., 2012 ; Wang et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Bifidobacterium Treated by Electrostatic Spray Drying Relieved Constipation by Changing the Relative Abundance of Bacteria Associated With Gastrointestinal Regulatory Peptides

Jiang

Liu

Fang

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

In this study, three different microencapsulation methods were used to embed Bifidobacterium to explore the alleviating effects of embedding methods on constipated mice. By measuring the defecation-related parameters, it was found that the Bifidobacteria treated by electrostatic spray drying had the best ability to relieved constipation. Furthermore, by detecting constipation-related gastrointestinal regulatory peptides, inflammatory factors, intestinal microbiota, and SCFAs, it was discovered that Bifidobacteria treated by electrostatic spray drying changed the composition of intestinal microbiota, especially the relative abundance of bacteria that were positively correlated with AQP3, but negatively correlated with ET-1 and SS, then increased the level of AQP3 in the intestine, and finally relieved constipation by increasing the fecal water content and small intestinal propulsion rate. In conclusion, the electrostatic spray drying method was superior to the other two methods in maintaining the activity of Bifidobacteria and relieved constipation by changing the relative abundance of bacteria that were correlated with gastrointestinal regulatory peptides and increasing the content of fecal water and small intestinal propulsion rate.

show abstract

“…The determination of the content of fecal water referred to the method of Wang et al. (2022) and was calculated by the following equation:…”

Section: Methodsmentioning

confidence: 99%

“…Before the end, mice were given activated carbon solution, and the time from the end of gavage to the detection of the first black stool was recorded ( Wang et al., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%